Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3103 and Pf6N2E2_3104 are separated by 173 nucleotides Pf6N2E2_3104 and Pf6N2E2_3105 are separated by 88 nucleotides Pf6N2E2_3105 and Pf6N2E2_3106 are separated by 34 nucleotides Pf6N2E2_3106 and Pf6N2E2_3107 are separated by 990 nucleotides
Pf6N2E2_3103 - Phosphoenolpyruvate carboxylase (EC 4.1.1.31), at 3,588,937 to 3,591,573
_3103
Pf6N2E2_3104 - FIG00958830: hypothetical protein, at 3,591,747 to 3,592,055
_3104
Pf6N2E2_3105 - hypothetical protein, at 3,592,144 to 3,592,464
_3105
Pf6N2E2_3106 - hypothetical protein, at 3,592,499 to 3,594,271
_3106
Pf6N2E2_3107 - unknown, without homologous in databases, at 3,595,262 to 3,596,917
_3107
Position (kb)
3592
3593
3594
3595 Strain fitness (log2 ratio)
-1
0
1
2 at 3591.514 kb on + strand at 3591.514 kb on + strand at 3591.607 kb on + strand at 3591.607 kb on + strand at 3591.607 kb on + strand at 3591.608 kb on - strand at 3591.654 kb on + strand at 3591.655 kb on - strand at 3591.655 kb on - strand at 3591.655 kb on - strand at 3591.674 kb on + strand at 3591.674 kb on + strand at 3591.675 kb on - strand at 3591.686 kb on + strand at 3591.687 kb on - strand at 3591.813 kb on + strand, within Pf6N2E2_3104 at 3591.870 kb on + strand, within Pf6N2E2_3104 at 3591.870 kb on + strand, within Pf6N2E2_3104 at 3591.870 kb on + strand, within Pf6N2E2_3104 at 3591.870 kb on + strand, within Pf6N2E2_3104 at 3591.871 kb on - strand, within Pf6N2E2_3104 at 3591.871 kb on - strand, within Pf6N2E2_3104 at 3591.871 kb on - strand, within Pf6N2E2_3104 at 3591.948 kb on + strand, within Pf6N2E2_3104 at 3591.948 kb on + strand, within Pf6N2E2_3104 at 3592.000 kb on - strand, within Pf6N2E2_3104 at 3592.022 kb on - strand, within Pf6N2E2_3104 at 3592.054 kb on - strand at 3592.107 kb on - strand at 3592.112 kb on + strand at 3592.112 kb on + strand at 3592.112 kb on + strand at 3592.112 kb on + strand at 3592.123 kb on + strand at 3592.124 kb on - strand at 3592.124 kb on - strand at 3592.124 kb on - strand at 3592.241 kb on + strand, within Pf6N2E2_3105 at 3592.270 kb on + strand, within Pf6N2E2_3105 at 3592.277 kb on + strand, within Pf6N2E2_3105 at 3592.277 kb on + strand, within Pf6N2E2_3105 at 3592.278 kb on - strand, within Pf6N2E2_3105 at 3592.317 kb on + strand, within Pf6N2E2_3105 at 3592.318 kb on - strand, within Pf6N2E2_3105 at 3592.319 kb on + strand, within Pf6N2E2_3105 at 3592.320 kb on - strand, within Pf6N2E2_3105 at 3592.320 kb on - strand, within Pf6N2E2_3105 at 3592.420 kb on + strand, within Pf6N2E2_3105 at 3592.420 kb on + strand, within Pf6N2E2_3105 at 3592.462 kb on + strand at 3592.484 kb on + strand at 3592.500 kb on + strand at 3592.527 kb on - strand at 3592.549 kb on - strand at 3592.574 kb on - strand at 3592.685 kb on - strand, within Pf6N2E2_3106 at 3592.834 kb on - strand, within Pf6N2E2_3106 at 3593.065 kb on + strand, within Pf6N2E2_3106 at 3593.396 kb on - strand, within Pf6N2E2_3106 at 3593.448 kb on + strand, within Pf6N2E2_3106 at 3593.480 kb on - strand, within Pf6N2E2_3106 at 3593.799 kb on - strand, within Pf6N2E2_3106 at 3593.849 kb on - strand, within Pf6N2E2_3106 at 3594.487 kb on + strand at 3594.638 kb on + strand at 3594.639 kb on - strand at 3594.661 kb on + strand at 3594.662 kb on - strand at 3594.662 kb on - strand at 3594.694 kb on - strand at 3594.748 kb on + strand at 3594.749 kb on - strand at 3594.763 kb on - strand at 3594.763 kb on - strand at 3594.903 kb on + strand at 3595.001 kb on + strand at 3595.009 kb on - strand at 3595.166 kb on - strand at 3595.166 kb on - strand at 3595.271 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 3,591,514 + -0.5 3,591,514 + +0.6 3,591,607 + -0.1 3,591,607 + -0.3 3,591,607 + -0.2 3,591,608 - -0.8 3,591,654 + +0.1 3,591,655 - +0.4 3,591,655 - +0.1 3,591,655 - +0.1 3,591,674 + +0.8 3,591,674 + +1.7 3,591,675 - +0.8 3,591,686 + -0.1 3,591,687 - +0.8 3,591,813 + Pf6N2E2_3104 0.21 +0.0 3,591,870 + Pf6N2E2_3104 0.40 +0.9 3,591,870 + Pf6N2E2_3104 0.40 +0.7 3,591,870 + Pf6N2E2_3104 0.40 -0.5 3,591,870 + Pf6N2E2_3104 0.40 +0.3 3,591,871 - Pf6N2E2_3104 0.40 +0.9 3,591,871 - Pf6N2E2_3104 0.40 +0.3 3,591,871 - Pf6N2E2_3104 0.40 +0.0 3,591,948 + Pf6N2E2_3104 0.65 +0.3 3,591,948 + Pf6N2E2_3104 0.65 -0.1 3,592,000 - Pf6N2E2_3104 0.82 -0.1 3,592,022 - Pf6N2E2_3104 0.89 +2.2 3,592,054 - -0.5 3,592,107 - +0.5 3,592,112 + -0.1 3,592,112 + -0.5 3,592,112 + -0.9 3,592,112 + +0.2 3,592,123 + +0.5 3,592,124 - -0.0 3,592,124 - -0.6 3,592,124 - -0.7 3,592,241 + Pf6N2E2_3105 0.30 -0.9 3,592,270 + Pf6N2E2_3105 0.39 -0.4 3,592,277 + Pf6N2E2_3105 0.41 +1.7 3,592,277 + Pf6N2E2_3105 0.41 -0.2 3,592,278 - Pf6N2E2_3105 0.42 -0.2 3,592,317 + Pf6N2E2_3105 0.54 -1.7 3,592,318 - Pf6N2E2_3105 0.54 +0.5 3,592,319 + Pf6N2E2_3105 0.55 +0.4 3,592,320 - Pf6N2E2_3105 0.55 +0.6 3,592,320 - Pf6N2E2_3105 0.55 +1.2 3,592,420 + Pf6N2E2_3105 0.86 -0.4 3,592,420 + Pf6N2E2_3105 0.86 -0.7 3,592,462 + +0.6 3,592,484 + +0.8 3,592,500 + +0.6 3,592,527 - +0.7 3,592,549 - +0.1 3,592,574 - +0.4 3,592,685 - Pf6N2E2_3106 0.10 -0.3 3,592,834 - Pf6N2E2_3106 0.19 -0.6 3,593,065 + Pf6N2E2_3106 0.32 -0.5 3,593,396 - Pf6N2E2_3106 0.51 -0.6 3,593,448 + Pf6N2E2_3106 0.54 -1.1 3,593,480 - Pf6N2E2_3106 0.55 -1.5 3,593,799 - Pf6N2E2_3106 0.73 +0.2 3,593,849 - Pf6N2E2_3106 0.76 -0.5 3,594,487 + +0.9 3,594,638 + +0.2 3,594,639 - +1.8 3,594,661 + -1.2 3,594,662 - +0.5 3,594,662 - +1.3 3,594,694 - +0.3 3,594,748 + -0.0 3,594,749 - -0.9 3,594,763 - +0.7 3,594,763 - -0.0 3,594,903 + +0.3 3,595,001 + +0.2 3,595,009 - +0.7 3,595,166 - +0.3 3,595,166 - -0.3 3,595,271 + +0.5
Or see this region's nucleotide sequence