Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2924 and Pf6N2E2_2925 overlap by 4 nucleotides Pf6N2E2_2925 and Pf6N2E2_2926 are separated by 0 nucleotides Pf6N2E2_2926 and Pf6N2E2_2927 are separated by 277 nucleotides
Pf6N2E2_2924 - ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, permease component 2 LivM (from data) , at 3,417,173 to 3,418,429
_2924
Pf6N2E2_2925 - ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, ATPase component 1 LivG (from data) , at 3,418,426 to 3,419,193
_2925
Pf6N2E2_2926 - ABC transporter for L-leucine/L-isoleucine/L-phenylalanine/D-alanine, ATPase component 2 LivF (from data) , at 3,419,194 to 3,419,895
_2926
Pf6N2E2_2927 - Putative signal peptide protein, at 3,420,173 to 3,420,478
_2927
Position (kb)
3418
3419
3420 Strain fitness (log2 ratio)
-1
0
1
2 at 3417.539 kb on + strand, within Pf6N2E2_2924 at 3417.539 kb on + strand, within Pf6N2E2_2924 at 3417.539 kb on + strand, within Pf6N2E2_2924 at 3417.539 kb on + strand, within Pf6N2E2_2924 at 3417.539 kb on + strand, within Pf6N2E2_2924 at 3417.539 kb on + strand, within Pf6N2E2_2924 at 3417.540 kb on - strand, within Pf6N2E2_2924 at 3417.540 kb on - strand, within Pf6N2E2_2924 at 3417.540 kb on - strand, within Pf6N2E2_2924 at 3417.574 kb on - strand, within Pf6N2E2_2924 at 3417.614 kb on + strand, within Pf6N2E2_2924 at 3417.614 kb on + strand, within Pf6N2E2_2924 at 3417.614 kb on + strand, within Pf6N2E2_2924 at 3417.630 kb on - strand, within Pf6N2E2_2924 at 3417.630 kb on - strand, within Pf6N2E2_2924 at 3417.635 kb on + strand, within Pf6N2E2_2924 at 3417.635 kb on + strand, within Pf6N2E2_2924 at 3417.635 kb on + strand, within Pf6N2E2_2924 at 3417.636 kb on - strand, within Pf6N2E2_2924 at 3417.636 kb on - strand, within Pf6N2E2_2924 at 3417.636 kb on - strand, within Pf6N2E2_2924 at 3417.636 kb on - strand, within Pf6N2E2_2924 at 3417.653 kb on + strand, within Pf6N2E2_2924 at 3417.653 kb on + strand, within Pf6N2E2_2924 at 3417.654 kb on - strand, within Pf6N2E2_2924 at 3417.654 kb on - strand, within Pf6N2E2_2924 at 3417.756 kb on - strand, within Pf6N2E2_2924 at 3417.756 kb on - strand, within Pf6N2E2_2924 at 3417.768 kb on + strand, within Pf6N2E2_2924 at 3417.768 kb on + strand, within Pf6N2E2_2924 at 3417.890 kb on - strand, within Pf6N2E2_2924 at 3417.924 kb on - strand, within Pf6N2E2_2924 at 3417.924 kb on - strand, within Pf6N2E2_2924 at 3417.938 kb on + strand, within Pf6N2E2_2924 at 3417.938 kb on + strand, within Pf6N2E2_2924 at 3417.938 kb on + strand, within Pf6N2E2_2924 at 3417.938 kb on + strand, within Pf6N2E2_2924 at 3417.938 kb on + strand, within Pf6N2E2_2924 at 3417.938 kb on + strand, within Pf6N2E2_2924 at 3417.939 kb on - strand, within Pf6N2E2_2924 at 3417.939 kb on - strand, within Pf6N2E2_2924 at 3417.939 kb on - strand, within Pf6N2E2_2924 at 3417.969 kb on - strand, within Pf6N2E2_2924 at 3417.969 kb on - strand, within Pf6N2E2_2924 at 3417.969 kb on - strand, within Pf6N2E2_2924 at 3418.397 kb on - strand at 3418.588 kb on + strand, within Pf6N2E2_2925 at 3418.588 kb on + strand, within Pf6N2E2_2925 at 3418.588 kb on + strand, within Pf6N2E2_2925 at 3418.588 kb on + strand, within Pf6N2E2_2925 at 3418.641 kb on - strand, within Pf6N2E2_2925 at 3418.673 kb on + strand, within Pf6N2E2_2925 at 3418.822 kb on + strand, within Pf6N2E2_2925 at 3419.191 kb on + strand at 3419.191 kb on + strand at 3419.191 kb on + strand at 3419.191 kb on + strand at 3419.192 kb on - strand at 3419.225 kb on - strand at 3419.371 kb on + strand, within Pf6N2E2_2926 at 3419.371 kb on + strand, within Pf6N2E2_2926 at 3419.453 kb on + strand, within Pf6N2E2_2926 at 3419.453 kb on + strand, within Pf6N2E2_2926 at 3419.454 kb on - strand, within Pf6N2E2_2926 at 3419.518 kb on + strand, within Pf6N2E2_2926 at 3419.519 kb on - strand, within Pf6N2E2_2926 at 3419.804 kb on - strand, within Pf6N2E2_2926 at 3419.804 kb on - strand, within Pf6N2E2_2926 at 3419.804 kb on - strand, within Pf6N2E2_2926 at 3419.882 kb on - strand at 3420.011 kb on - strand at 3420.091 kb on + strand at 3420.115 kb on + strand at 3420.115 kb on + strand at 3420.116 kb on - strand at 3420.116 kb on - strand at 3420.116 kb on - strand at 3420.116 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 3,417,539 + Pf6N2E2_2924 0.29 -0.2 3,417,539 + Pf6N2E2_2924 0.29 -0.3 3,417,539 + Pf6N2E2_2924 0.29 +0.4 3,417,539 + Pf6N2E2_2924 0.29 -0.2 3,417,539 + Pf6N2E2_2924 0.29 -0.3 3,417,539 + Pf6N2E2_2924 0.29 -0.2 3,417,540 - Pf6N2E2_2924 0.29 +0.8 3,417,540 - Pf6N2E2_2924 0.29 -0.6 3,417,540 - Pf6N2E2_2924 0.29 +2.3 3,417,574 - Pf6N2E2_2924 0.32 +0.0 3,417,614 + Pf6N2E2_2924 0.35 +0.3 3,417,614 + Pf6N2E2_2924 0.35 +1.2 3,417,614 + Pf6N2E2_2924 0.35 -0.4 3,417,630 - Pf6N2E2_2924 0.36 +0.1 3,417,630 - Pf6N2E2_2924 0.36 -0.3 3,417,635 + Pf6N2E2_2924 0.37 +0.6 3,417,635 + Pf6N2E2_2924 0.37 -0.8 3,417,635 + Pf6N2E2_2924 0.37 -0.4 3,417,636 - Pf6N2E2_2924 0.37 -0.7 3,417,636 - Pf6N2E2_2924 0.37 +0.4 3,417,636 - Pf6N2E2_2924 0.37 +0.3 3,417,636 - Pf6N2E2_2924 0.37 -0.5 3,417,653 + Pf6N2E2_2924 0.38 +0.0 3,417,653 + Pf6N2E2_2924 0.38 +0.5 3,417,654 - Pf6N2E2_2924 0.38 -0.9 3,417,654 - Pf6N2E2_2924 0.38 +0.7 3,417,756 - Pf6N2E2_2924 0.46 +1.6 3,417,756 - Pf6N2E2_2924 0.46 -0.3 3,417,768 + Pf6N2E2_2924 0.47 -0.3 3,417,768 + Pf6N2E2_2924 0.47 -0.3 3,417,890 - Pf6N2E2_2924 0.57 +0.5 3,417,924 - Pf6N2E2_2924 0.60 +0.3 3,417,924 - Pf6N2E2_2924 0.60 -1.0 3,417,938 + Pf6N2E2_2924 0.61 -0.1 3,417,938 + Pf6N2E2_2924 0.61 -0.2 3,417,938 + Pf6N2E2_2924 0.61 +0.5 3,417,938 + Pf6N2E2_2924 0.61 -0.5 3,417,938 + Pf6N2E2_2924 0.61 -0.1 3,417,938 + Pf6N2E2_2924 0.61 -1.0 3,417,939 - Pf6N2E2_2924 0.61 +0.0 3,417,939 - Pf6N2E2_2924 0.61 +0.8 3,417,939 - Pf6N2E2_2924 0.61 -0.1 3,417,969 - Pf6N2E2_2924 0.63 +0.2 3,417,969 - Pf6N2E2_2924 0.63 +0.3 3,417,969 - Pf6N2E2_2924 0.63 -0.2 3,418,397 - +0.5 3,418,588 + Pf6N2E2_2925 0.21 -0.2 3,418,588 + Pf6N2E2_2925 0.21 -0.6 3,418,588 + Pf6N2E2_2925 0.21 -0.1 3,418,588 + Pf6N2E2_2925 0.21 +0.5 3,418,641 - Pf6N2E2_2925 0.28 -0.8 3,418,673 + Pf6N2E2_2925 0.32 -1.4 3,418,822 + Pf6N2E2_2925 0.52 -0.7 3,419,191 + +0.0 3,419,191 + +0.3 3,419,191 + +0.1 3,419,191 + -0.8 3,419,192 - +0.3 3,419,225 - +0.9 3,419,371 + Pf6N2E2_2926 0.25 -1.3 3,419,371 + Pf6N2E2_2926 0.25 +0.2 3,419,453 + Pf6N2E2_2926 0.37 +0.6 3,419,453 + Pf6N2E2_2926 0.37 +0.8 3,419,454 - Pf6N2E2_2926 0.37 +0.3 3,419,518 + Pf6N2E2_2926 0.46 -0.0 3,419,519 - Pf6N2E2_2926 0.46 +0.7 3,419,804 - Pf6N2E2_2926 0.87 +0.1 3,419,804 - Pf6N2E2_2926 0.87 -0.1 3,419,804 - Pf6N2E2_2926 0.87 -0.1 3,419,882 - +0.0 3,420,011 - +0.1 3,420,091 + +1.2 3,420,115 + +0.2 3,420,115 + -1.2 3,420,116 - -0.2 3,420,116 - +1.1 3,420,116 - +0.4 3,420,116 - +0.7
Or see this region's nucleotide sequence