Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_284 and Pf6N2E2_285 are separated by 248 nucleotides Pf6N2E2_285 and Pf6N2E2_286 overlap by 4 nucleotides
Pf6N2E2_284 - Adenylosuccinate lyase (EC 4.3.2.2), at 350,256 to 351,626
_284
Pf6N2E2_285 - FIG002903: a protein of unknown function perhaps involved in purine metabolism, at 351,875 to 352,498
_285
Pf6N2E2_286 - tRNA-specific 2-thiouridylase MnmA, at 352,495 to 353,619
_286
Position (kb) 351
352
353 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 351.713 kb on - strand at 351.732 kb on + strand at 351.732 kb on + strand at 351.732 kb on + strand at 351.732 kb on + strand at 351.733 kb on - strand at 351.733 kb on - strand at 351.733 kb on - strand at 351.733 kb on - strand at 351.733 kb on - strand at 351.733 kb on - strand at 351.733 kb on - strand at 351.736 kb on + strand at 351.736 kb on + strand at 351.736 kb on + strand at 351.737 kb on - strand at 351.737 kb on - strand at 351.737 kb on - strand at 351.737 kb on - strand at 351.739 kb on + strand at 351.739 kb on + strand at 351.740 kb on - strand at 351.876 kb on + strand at 351.876 kb on + strand at 351.877 kb on - strand at 352.075 kb on + strand, within Pf6N2E2_285 at 352.093 kb on - strand, within Pf6N2E2_285 at 352.242 kb on + strand, within Pf6N2E2_285 at 352.306 kb on - strand, within Pf6N2E2_285 at 352.338 kb on + strand, within Pf6N2E2_285 at 352.339 kb on - strand, within Pf6N2E2_285 at 352.394 kb on + strand, within Pf6N2E2_285
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 351,713 - -0.3 351,732 + -0.8 351,732 + -0.1 351,732 + -0.3 351,732 + -0.2 351,733 - -0.1 351,733 - +0.0 351,733 - -0.9 351,733 - -1.5 351,733 - -1.3 351,733 - -0.7 351,733 - -0.0 351,736 + -0.1 351,736 + -0.1 351,736 + -0.8 351,737 - -1.7 351,737 - +0.2 351,737 - +0.3 351,737 - -0.4 351,739 + -0.8 351,739 + +0.6 351,740 - -0.4 351,876 + +0.2 351,876 + +1.0 351,877 - +0.3 352,075 + Pf6N2E2_285 0.32 -0.6 352,093 - Pf6N2E2_285 0.35 -1.1 352,242 + Pf6N2E2_285 0.59 -0.2 352,306 - Pf6N2E2_285 0.69 -1.8 352,338 + Pf6N2E2_285 0.74 -1.3 352,339 - Pf6N2E2_285 0.74 -3.7 352,394 + Pf6N2E2_285 0.83 -2.3
Or see this region's nucleotide sequence