Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2360 and Pf6N2E2_2361 are separated by 47 nucleotides Pf6N2E2_2361 and Pf6N2E2_2362 are separated by 475 nucleotides
Pf6N2E2_2360 - cell invasion protein SipB, at 2,794,599 to 2,796,335
_2360
Pf6N2E2_2361 - Type III secretion chaperone protein for YopD (SycD), at 2,796,383 to 2,796,865
_2361
Pf6N2E2_2362 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 2,797,341 to 2,798,375
_2362
Position (kb)
2796
2797 Strain fitness (log2 ratio)
-2
-1
0
1 at 2795.476 kb on + strand at 2795.477 kb on - strand, within Pf6N2E2_2360 at 2795.477 kb on - strand, within Pf6N2E2_2360 at 2795.480 kb on + strand, within Pf6N2E2_2360 at 2795.481 kb on - strand, within Pf6N2E2_2360 at 2795.500 kb on + strand, within Pf6N2E2_2360 at 2795.500 kb on + strand, within Pf6N2E2_2360 at 2795.501 kb on - strand, within Pf6N2E2_2360 at 2795.717 kb on + strand, within Pf6N2E2_2360 at 2795.718 kb on - strand, within Pf6N2E2_2360 at 2795.718 kb on - strand, within Pf6N2E2_2360 at 2795.718 kb on - strand, within Pf6N2E2_2360 at 2796.188 kb on + strand at 2796.221 kb on - strand at 2796.268 kb on + strand at 2796.269 kb on - strand at 2796.269 kb on - strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.369 kb on + strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.370 kb on - strand at 2796.429 kb on + strand at 2796.430 kb on - strand at 2796.430 kb on - strand at 2796.430 kb on - strand at 2796.430 kb on - strand at 2796.480 kb on + strand, within Pf6N2E2_2361 at 2796.481 kb on - strand, within Pf6N2E2_2361 at 2796.481 kb on - strand, within Pf6N2E2_2361 at 2796.481 kb on - strand, within Pf6N2E2_2361 at 2796.540 kb on + strand, within Pf6N2E2_2361 at 2796.541 kb on - strand, within Pf6N2E2_2361 at 2796.589 kb on - strand, within Pf6N2E2_2361 at 2796.589 kb on - strand, within Pf6N2E2_2361 at 2796.651 kb on + strand, within Pf6N2E2_2361 at 2796.663 kb on + strand, within Pf6N2E2_2361 at 2796.663 kb on + strand, within Pf6N2E2_2361 at 2796.663 kb on + strand, within Pf6N2E2_2361 at 2796.663 kb on + strand, within Pf6N2E2_2361 at 2796.664 kb on - strand, within Pf6N2E2_2361 at 2796.673 kb on - strand, within Pf6N2E2_2361 at 2796.726 kb on + strand, within Pf6N2E2_2361 at 2796.726 kb on + strand, within Pf6N2E2_2361 at 2796.727 kb on - strand, within Pf6N2E2_2361 at 2796.727 kb on - strand, within Pf6N2E2_2361 at 2796.727 kb on - strand, within Pf6N2E2_2361 at 2796.727 kb on - strand, within Pf6N2E2_2361 at 2796.747 kb on + strand, within Pf6N2E2_2361 at 2797.249 kb on + strand at 2797.417 kb on + strand at 2797.417 kb on + strand at 2797.492 kb on + strand, within Pf6N2E2_2362 at 2797.492 kb on + strand, within Pf6N2E2_2362 at 2797.492 kb on + strand, within Pf6N2E2_2362 at 2797.570 kb on + strand, within Pf6N2E2_2362 at 2797.570 kb on + strand, within Pf6N2E2_2362 at 2797.766 kb on - strand, within Pf6N2E2_2362
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 2,795,476 + +1.2 2,795,477 - Pf6N2E2_2360 0.51 -0.8 2,795,477 - Pf6N2E2_2360 0.51 -0.6 2,795,480 + Pf6N2E2_2360 0.51 -0.7 2,795,481 - Pf6N2E2_2360 0.51 -0.4 2,795,500 + Pf6N2E2_2360 0.52 -0.7 2,795,500 + Pf6N2E2_2360 0.52 +0.2 2,795,501 - Pf6N2E2_2360 0.52 -0.7 2,795,717 + Pf6N2E2_2360 0.64 +0.2 2,795,718 - Pf6N2E2_2360 0.64 +1.6 2,795,718 - Pf6N2E2_2360 0.64 -0.6 2,795,718 - Pf6N2E2_2360 0.64 -0.8 2,796,188 + -0.2 2,796,221 - +0.6 2,796,268 + -0.9 2,796,269 - +0.3 2,796,269 - +0.9 2,796,369 + -0.7 2,796,369 + -0.6 2,796,369 + +0.1 2,796,369 + +0.3 2,796,369 + -0.1 2,796,369 + +0.3 2,796,370 - +0.3 2,796,370 - +0.4 2,796,370 - -0.2 2,796,370 - -0.1 2,796,370 - -0.6 2,796,370 - +0.5 2,796,429 + -0.8 2,796,430 - -0.7 2,796,430 - -0.1 2,796,430 - -0.9 2,796,430 - -2.1 2,796,480 + Pf6N2E2_2361 0.20 -0.6 2,796,481 - Pf6N2E2_2361 0.20 -1.4 2,796,481 - Pf6N2E2_2361 0.20 -0.1 2,796,481 - Pf6N2E2_2361 0.20 -1.1 2,796,540 + Pf6N2E2_2361 0.33 +0.8 2,796,541 - Pf6N2E2_2361 0.33 -1.8 2,796,589 - Pf6N2E2_2361 0.43 -0.2 2,796,589 - Pf6N2E2_2361 0.43 +0.2 2,796,651 + Pf6N2E2_2361 0.55 -1.0 2,796,663 + Pf6N2E2_2361 0.58 +0.2 2,796,663 + Pf6N2E2_2361 0.58 -1.4 2,796,663 + Pf6N2E2_2361 0.58 -1.6 2,796,663 + Pf6N2E2_2361 0.58 -1.8 2,796,664 - Pf6N2E2_2361 0.58 -0.1 2,796,673 - Pf6N2E2_2361 0.60 -0.4 2,796,726 + Pf6N2E2_2361 0.71 +0.4 2,796,726 + Pf6N2E2_2361 0.71 -1.0 2,796,727 - Pf6N2E2_2361 0.71 +0.1 2,796,727 - Pf6N2E2_2361 0.71 -0.7 2,796,727 - Pf6N2E2_2361 0.71 -0.5 2,796,727 - Pf6N2E2_2361 0.71 -0.4 2,796,747 + Pf6N2E2_2361 0.75 +0.5 2,797,249 + +0.1 2,797,417 + -0.1 2,797,417 + -0.5 2,797,492 + Pf6N2E2_2362 0.15 -0.4 2,797,492 + Pf6N2E2_2362 0.15 -0.2 2,797,492 + Pf6N2E2_2362 0.15 +0.3 2,797,570 + Pf6N2E2_2362 0.22 -1.2 2,797,570 + Pf6N2E2_2362 0.22 -1.4 2,797,766 - Pf6N2E2_2362 0.41 -1.1
Or see this region's nucleotide sequence