Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1712 and Pf6N2E2_1713 are separated by 5 nucleotides Pf6N2E2_1713 and Pf6N2E2_1714 are separated by 3 nucleotides Pf6N2E2_1714 and Pf6N2E2_1715 are separated by 1 nucleotides Pf6N2E2_1715 and Pf6N2E2_1716 are separated by 8 nucleotides
Pf6N2E2_1712 - Permease of the drug/metabolite transporter (DMT) superfamily, at 2,020,844 to 2,021,749
_1712
Pf6N2E2_1713 - iron-sulfur flavoprotein, at 2,021,755 to 2,022,366
_1713
Pf6N2E2_1714 - FIG01218476: hypothetical protein, at 2,022,370 to 2,023,053
_1714
Pf6N2E2_1715 - hypothetical protein, at 2,023,055 to 2,023,600
_1715
Pf6N2E2_1716 - Phenylacetate-coenzyme A ligase (EC 6.2.1.30), at 2,023,609 to 2,024,961
_1716
Position (kb)
2022
2023
2024 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2021.488 kb on + strand, within Pf6N2E2_1712 at 2021.488 kb on + strand, within Pf6N2E2_1712 at 2021.488 kb on + strand, within Pf6N2E2_1712 at 2021.518 kb on - strand, within Pf6N2E2_1712 at 2022.134 kb on + strand, within Pf6N2E2_1713 at 2022.407 kb on + strand at 2022.520 kb on - strand, within Pf6N2E2_1714 at 2022.638 kb on + strand, within Pf6N2E2_1714 at 2022.662 kb on + strand, within Pf6N2E2_1714 at 2022.912 kb on - strand, within Pf6N2E2_1714 at 2022.912 kb on - strand, within Pf6N2E2_1714 at 2022.917 kb on + strand, within Pf6N2E2_1714 at 2022.942 kb on - strand, within Pf6N2E2_1714 at 2023.102 kb on - strand at 2023.182 kb on + strand, within Pf6N2E2_1715 at 2023.183 kb on - strand, within Pf6N2E2_1715 at 2023.446 kb on + strand, within Pf6N2E2_1715 at 2023.447 kb on - strand, within Pf6N2E2_1715 at 2023.527 kb on + strand, within Pf6N2E2_1715
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 2,021,488 + Pf6N2E2_1712 0.71 +0.2 2,021,488 + Pf6N2E2_1712 0.71 -0.5 2,021,488 + Pf6N2E2_1712 0.71 -0.2 2,021,518 - Pf6N2E2_1712 0.74 -1.1 2,022,134 + Pf6N2E2_1713 0.62 +0.6 2,022,407 + +2.3 2,022,520 - Pf6N2E2_1714 0.22 -0.9 2,022,638 + Pf6N2E2_1714 0.39 -0.0 2,022,662 + Pf6N2E2_1714 0.43 -0.8 2,022,912 - Pf6N2E2_1714 0.79 -1.1 2,022,912 - Pf6N2E2_1714 0.79 -0.4 2,022,917 + Pf6N2E2_1714 0.80 -1.9 2,022,942 - Pf6N2E2_1714 0.84 -0.0 2,023,102 - -1.2 2,023,182 + Pf6N2E2_1715 0.23 +0.2 2,023,183 - Pf6N2E2_1715 0.23 -0.3 2,023,446 + Pf6N2E2_1715 0.72 -0.4 2,023,447 - Pf6N2E2_1715 0.72 +0.1 2,023,527 + Pf6N2E2_1715 0.86 +0.3
Or see this region's nucleotide sequence