Experiment: Spectinomycin 0.01 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_1657 and Pf6N2E2_1658 are separated by 78 nucleotides Pf6N2E2_1658 and Pf6N2E2_1659 are separated by 18 nucleotides Pf6N2E2_1659 and Pf6N2E2_1660 are separated by 113 nucleotides Pf6N2E2_1660 and Pf6N2E2_1661 are separated by 3 nucleotides 
        Pf6N2E2_1657 - NAD glycohydrolase, hvnA; Halovibrin, at 1,962,761 to 1,963,996 
        _1657 
         
        
        Pf6N2E2_1658 - hypothetical protein, at 1,964,075 to 1,964,323 
        _1658 
         
        
        Pf6N2E2_1659 - Inositol transport system sugar-binding protein, at 1,964,342 to 1,964,455 
        _1659 
         
        
        Pf6N2E2_1660 - RND efflux system, membrane fusion protein CmeA, at 1,964,569 to 1,965,738 
        _1660 
         
        
        Pf6N2E2_1661 - RND efflux system, inner membrane transporter CmeB, at 1,965,742 to 1,968,837 
        _1661 
         Position (kb) 1964 
1965 
1966 Strain fitness (log2 ratio) -4 
-3 
-2 
-1 
0 
1 at 1963.664 kb on + strand, within Pf6N2E2_1657 at 1963.665 kb on - strand, within Pf6N2E2_1657 at 1963.678 kb on + strand, within Pf6N2E2_1657 at 1963.679 kb on - strand, within Pf6N2E2_1657 at 1963.679 kb on - strand, within Pf6N2E2_1657 at 1963.799 kb on + strand, within Pf6N2E2_1657 at 1963.799 kb on + strand, within Pf6N2E2_1657 at 1963.799 kb on + strand, within Pf6N2E2_1657 at 1963.835 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.949 kb on + strand at 1963.949 kb on + strand at 1963.950 kb on - strand at 1963.979 kb on + strand at 1963.980 kb on - strand at 1963.980 kb on - strand at 1964.001 kb on + strand at 1964.097 kb on - strand at 1964.302 kb on - strand at 1964.313 kb on - strand at 1964.313 kb on - strand at 1964.366 kb on - strand, within Pf6N2E2_1659 at 1964.481 kb on - strand at 1964.481 kb on - strand at 1964.502 kb on - strand at 1964.723 kb on + strand, within Pf6N2E2_1660 at 1964.723 kb on + strand, within Pf6N2E2_1660 at 1964.723 kb on + strand, within Pf6N2E2_1660 at 1965.105 kb on + strand, within Pf6N2E2_1660 at 1965.736 kb on + strand at 1965.736 kb on + strand at 1965.737 kb on - strand at 1965.737 kb on - strand at 1965.737 kb on - strand at 1965.877 kb on + strand at 1965.970 kb on + strand at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.562 kb on - strand, within Pf6N2E2_1661 at 1966.684 kb on + strand, within Pf6N2E2_1661 at 1966.685 kb on - strand, within Pf6N2E2_1661 at 1966.705 kb on + strand, within Pf6N2E2_1661  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Spectinomycin 0.01 mg/ml remove 1,963,664 +  Pf6N2E2_1657 0.73  +0.2 1,963,665 -  Pf6N2E2_1657 0.73  +0.5 1,963,678 +  Pf6N2E2_1657 0.74  -0.1 1,963,679 -  Pf6N2E2_1657 0.74  +0.5 1,963,679 -  Pf6N2E2_1657 0.74  -0.5 1,963,799 +  Pf6N2E2_1657 0.84  +0.5 1,963,799 +  Pf6N2E2_1657 0.84  -0.2 1,963,799 +  Pf6N2E2_1657 0.84  -0.0 1,963,835 +  Pf6N2E2_1657 0.87  +0.5 1,963,869 +  Pf6N2E2_1657 0.90  +0.2 1,963,869 +  Pf6N2E2_1657 0.90  +1.2 1,963,869 +  Pf6N2E2_1657 0.90  +0.8 1,963,869 +  Pf6N2E2_1657 0.90  -0.4 1,963,869 +  Pf6N2E2_1657 0.90  +0.1 1,963,869 +  Pf6N2E2_1657 0.90  -0.6 1,963,869 +  Pf6N2E2_1657 0.90  -0.5 1,963,870 -  Pf6N2E2_1657 0.90  -0.5 1,963,870 -  Pf6N2E2_1657 0.90  -0.1 1,963,870 -  Pf6N2E2_1657 0.90  +0.3 1,963,870 -  Pf6N2E2_1657 0.90  -1.5 1,963,870 -  Pf6N2E2_1657 0.90  +0.2 1,963,870 -  Pf6N2E2_1657 0.90  -0.5 1,963,870 -  Pf6N2E2_1657 0.90  +0.7 1,963,870 -  Pf6N2E2_1657 0.90  -0.0 1,963,870 -  Pf6N2E2_1657 0.90  -1.0 1,963,870 -  Pf6N2E2_1657 0.90  +0.8 1,963,949 +  +0.9 1,963,949 +  -0.0 1,963,950 -  +0.4 1,963,979 +  -0.4 1,963,980 -  -0.9 1,963,980 -  -0.1 1,964,001 +  +0.8 1,964,097 -  +0.1 1,964,302 -  -0.2 1,964,313 -  -0.2 1,964,313 -  +0.2 1,964,366 -  Pf6N2E2_1659 0.21  -0.7 1,964,481 -  -0.4 1,964,481 -  -1.6 1,964,502 -  -3.4 1,964,723 +  Pf6N2E2_1660 0.13  -4.2 1,964,723 +  Pf6N2E2_1660 0.13  -1.1 1,964,723 +  Pf6N2E2_1660 0.13  -1.7 1,965,105 +  Pf6N2E2_1660 0.46  -2.7 1,965,736 +  -1.1 1,965,736 +  -2.5 1,965,737 -  -3.5 1,965,737 -  -2.7 1,965,737 -  -1.1 1,965,877 +  -2.4 1,965,970 +  -4.1 1,966,485 -  Pf6N2E2_1661 0.24  -1.4 1,966,485 -  Pf6N2E2_1661 0.24  -3.0 1,966,485 -  Pf6N2E2_1661 0.24  -3.4 1,966,485 -  Pf6N2E2_1661 0.24  -3.0 1,966,562 -  Pf6N2E2_1661 0.26  -2.9 1,966,684 +  Pf6N2E2_1661 0.30  -1.9 1,966,685 -  Pf6N2E2_1661 0.30  -4.1 1,966,705 +  Pf6N2E2_1661 0.31  -3.0 
Or see this region's nucleotide sequence