Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1194 and Pf6N2E2_1195 are separated by 29 nucleotides Pf6N2E2_1195 and Pf6N2E2_1196 are separated by 22 nucleotides
Pf6N2E2_1194 - hypothetical protein, at 1,396,443 to 1,397,384
_1194
Pf6N2E2_1195 - hypothetical protein, at 1,397,414 to 1,397,809
_1195
Pf6N2E2_1196 - hypothetical protein, at 1,397,832 to 1,399,154
_1196
Position (kb)
1397
1398 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1396.437 kb on - strand at 1396.509 kb on + strand at 1396.529 kb on + strand at 1396.614 kb on - strand, within Pf6N2E2_1194 at 1396.753 kb on - strand, within Pf6N2E2_1194 at 1396.798 kb on + strand, within Pf6N2E2_1194 at 1396.798 kb on + strand, within Pf6N2E2_1194 at 1396.799 kb on - strand, within Pf6N2E2_1194 at 1396.807 kb on - strand, within Pf6N2E2_1194 at 1396.894 kb on + strand, within Pf6N2E2_1194 at 1396.895 kb on - strand, within Pf6N2E2_1194 at 1396.895 kb on - strand, within Pf6N2E2_1194 at 1396.928 kb on - strand, within Pf6N2E2_1194 at 1397.006 kb on - strand, within Pf6N2E2_1194 at 1397.006 kb on - strand, within Pf6N2E2_1194 at 1397.006 kb on - strand, within Pf6N2E2_1194 at 1397.029 kb on - strand, within Pf6N2E2_1194 at 1397.167 kb on - strand, within Pf6N2E2_1194 at 1397.223 kb on + strand, within Pf6N2E2_1194 at 1397.223 kb on + strand, within Pf6N2E2_1194 at 1397.223 kb on + strand, within Pf6N2E2_1194 at 1397.383 kb on - strand at 1397.537 kb on - strand, within Pf6N2E2_1195 at 1397.537 kb on - strand, within Pf6N2E2_1195 at 1397.681 kb on + strand, within Pf6N2E2_1195 at 1397.682 kb on - strand, within Pf6N2E2_1195 at 1397.682 kb on - strand, within Pf6N2E2_1195 at 1397.682 kb on - strand, within Pf6N2E2_1195 at 1397.682 kb on - strand, within Pf6N2E2_1195 at 1397.687 kb on - strand, within Pf6N2E2_1195 at 1397.696 kb on - strand, within Pf6N2E2_1195 at 1397.797 kb on + strand at 1397.837 kb on + strand at 1397.838 kb on - strand at 1397.838 kb on - strand at 1397.838 kb on - strand at 1397.840 kb on + strand at 1397.841 kb on - strand at 1397.990 kb on - strand, within Pf6N2E2_1196 at 1397.990 kb on - strand, within Pf6N2E2_1196 at 1397.990 kb on - strand, within Pf6N2E2_1196 at 1398.222 kb on - strand, within Pf6N2E2_1196 at 1398.312 kb on - strand, within Pf6N2E2_1196 at 1398.312 kb on - strand, within Pf6N2E2_1196 at 1398.416 kb on + strand, within Pf6N2E2_1196 at 1398.417 kb on - strand, within Pf6N2E2_1196 at 1398.499 kb on - strand, within Pf6N2E2_1196 at 1398.534 kb on + strand, within Pf6N2E2_1196 at 1398.547 kb on - strand, within Pf6N2E2_1196 at 1398.639 kb on - strand, within Pf6N2E2_1196 at 1398.657 kb on + strand, within Pf6N2E2_1196 at 1398.658 kb on - strand, within Pf6N2E2_1196 at 1398.779 kb on - strand, within Pf6N2E2_1196
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 1,396,437 - +0.3 1,396,509 + -0.6 1,396,529 + -0.0 1,396,614 - Pf6N2E2_1194 0.18 +0.4 1,396,753 - Pf6N2E2_1194 0.33 +0.1 1,396,798 + Pf6N2E2_1194 0.38 +0.1 1,396,798 + Pf6N2E2_1194 0.38 +1.1 1,396,799 - Pf6N2E2_1194 0.38 +0.2 1,396,807 - Pf6N2E2_1194 0.39 +1.2 1,396,894 + Pf6N2E2_1194 0.48 +1.3 1,396,895 - Pf6N2E2_1194 0.48 -0.2 1,396,895 - Pf6N2E2_1194 0.48 -1.1 1,396,928 - Pf6N2E2_1194 0.51 -0.0 1,397,006 - Pf6N2E2_1194 0.60 +0.4 1,397,006 - Pf6N2E2_1194 0.60 -0.4 1,397,006 - Pf6N2E2_1194 0.60 +0.4 1,397,029 - Pf6N2E2_1194 0.62 +0.2 1,397,167 - Pf6N2E2_1194 0.77 -0.9 1,397,223 + Pf6N2E2_1194 0.83 +0.4 1,397,223 + Pf6N2E2_1194 0.83 -1.1 1,397,223 + Pf6N2E2_1194 0.83 +0.8 1,397,383 - +1.2 1,397,537 - Pf6N2E2_1195 0.31 -0.2 1,397,537 - Pf6N2E2_1195 0.31 +1.6 1,397,681 + Pf6N2E2_1195 0.67 -1.4 1,397,682 - Pf6N2E2_1195 0.68 -1.1 1,397,682 - Pf6N2E2_1195 0.68 -0.5 1,397,682 - Pf6N2E2_1195 0.68 -1.3 1,397,682 - Pf6N2E2_1195 0.68 -1.0 1,397,687 - Pf6N2E2_1195 0.69 -0.8 1,397,696 - Pf6N2E2_1195 0.71 +0.3 1,397,797 + -1.0 1,397,837 + -0.3 1,397,838 - -0.2 1,397,838 - +0.5 1,397,838 - +0.8 1,397,840 + +1.8 1,397,841 - +0.3 1,397,990 - Pf6N2E2_1196 0.12 +1.2 1,397,990 - Pf6N2E2_1196 0.12 -0.6 1,397,990 - Pf6N2E2_1196 0.12 -2.0 1,398,222 - Pf6N2E2_1196 0.29 +0.4 1,398,312 - Pf6N2E2_1196 0.36 +0.1 1,398,312 - Pf6N2E2_1196 0.36 -0.9 1,398,416 + Pf6N2E2_1196 0.44 +0.9 1,398,417 - Pf6N2E2_1196 0.44 +0.1 1,398,499 - Pf6N2E2_1196 0.50 -1.6 1,398,534 + Pf6N2E2_1196 0.53 -0.8 1,398,547 - Pf6N2E2_1196 0.54 -0.3 1,398,639 - Pf6N2E2_1196 0.61 +0.1 1,398,657 + Pf6N2E2_1196 0.62 -3.5 1,398,658 - Pf6N2E2_1196 0.62 +0.4 1,398,779 - Pf6N2E2_1196 0.72 +1.0
Or see this region's nucleotide sequence