Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1187 and Pf6N2E2_1188 are separated by 9 nucleotides Pf6N2E2_1188 and Pf6N2E2_1189 overlap by 10 nucleotides
Pf6N2E2_1187 - Probable nikkomycin biosynthesis protein, carboxylase, at 1,389,990 to 1,391,273
_1187
Pf6N2E2_1188 - Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17), at 1,391,283 to 1,392,140
_1188
Pf6N2E2_1189 - Thioredoxin reductase (EC 1.8.1.9), at 1,392,131 to 1,393,300
_1189
Position (kb)
1391
1392
1393 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1390.655 kb on - strand, within Pf6N2E2_1187 at 1390.805 kb on - strand, within Pf6N2E2_1187 at 1390.805 kb on - strand, within Pf6N2E2_1187 at 1390.831 kb on - strand, within Pf6N2E2_1187 at 1390.932 kb on - strand, within Pf6N2E2_1187 at 1390.941 kb on + strand, within Pf6N2E2_1187 at 1390.941 kb on + strand, within Pf6N2E2_1187 at 1390.942 kb on - strand, within Pf6N2E2_1187 at 1391.004 kb on - strand, within Pf6N2E2_1187 at 1391.018 kb on + strand, within Pf6N2E2_1187 at 1391.018 kb on + strand, within Pf6N2E2_1187 at 1391.019 kb on - strand, within Pf6N2E2_1187 at 1391.019 kb on - strand, within Pf6N2E2_1187 at 1391.135 kb on + strand, within Pf6N2E2_1187 at 1391.135 kb on + strand, within Pf6N2E2_1187 at 1391.171 kb on - strand at 1391.234 kb on + strand at 1391.239 kb on + strand at 1391.239 kb on + strand at 1391.273 kb on + strand at 1391.273 kb on + strand at 1391.274 kb on - strand at 1391.276 kb on + strand at 1391.276 kb on + strand at 1391.276 kb on + strand at 1391.276 kb on + strand at 1391.277 kb on - strand at 1391.277 kb on - strand at 1391.360 kb on + strand at 1391.496 kb on + strand, within Pf6N2E2_1188 at 1391.524 kb on + strand, within Pf6N2E2_1188 at 1391.525 kb on - strand, within Pf6N2E2_1188 at 1391.525 kb on - strand, within Pf6N2E2_1188 at 1391.558 kb on - strand, within Pf6N2E2_1188 at 1391.803 kb on - strand, within Pf6N2E2_1188 at 1391.863 kb on + strand, within Pf6N2E2_1188 at 1392.004 kb on + strand, within Pf6N2E2_1188 at 1392.004 kb on + strand, within Pf6N2E2_1188 at 1392.005 kb on - strand, within Pf6N2E2_1188 at 1392.005 kb on - strand, within Pf6N2E2_1188 at 1392.043 kb on - strand, within Pf6N2E2_1188 at 1392.281 kb on - strand, within Pf6N2E2_1189 at 1392.281 kb on - strand, within Pf6N2E2_1189 at 1392.534 kb on + strand, within Pf6N2E2_1189 at 1392.535 kb on - strand, within Pf6N2E2_1189 at 1392.551 kb on - strand, within Pf6N2E2_1189 at 1392.551 kb on - strand, within Pf6N2E2_1189 at 1392.683 kb on + strand, within Pf6N2E2_1189 at 1392.684 kb on - strand, within Pf6N2E2_1189 at 1392.831 kb on + strand, within Pf6N2E2_1189 at 1393.047 kb on + strand, within Pf6N2E2_1189 at 1393.048 kb on - strand, within Pf6N2E2_1189 at 1393.056 kb on + strand, within Pf6N2E2_1189 at 1393.057 kb on - strand, within Pf6N2E2_1189 at 1393.110 kb on + strand, within Pf6N2E2_1189
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 1,390,655 - Pf6N2E2_1187 0.52 -0.1 1,390,805 - Pf6N2E2_1187 0.63 +0.5 1,390,805 - Pf6N2E2_1187 0.63 +1.9 1,390,831 - Pf6N2E2_1187 0.65 +0.4 1,390,932 - Pf6N2E2_1187 0.73 -0.2 1,390,941 + Pf6N2E2_1187 0.74 +1.0 1,390,941 + Pf6N2E2_1187 0.74 +0.2 1,390,942 - Pf6N2E2_1187 0.74 -1.0 1,391,004 - Pf6N2E2_1187 0.79 +0.4 1,391,018 + Pf6N2E2_1187 0.80 +0.2 1,391,018 + Pf6N2E2_1187 0.80 +0.2 1,391,019 - Pf6N2E2_1187 0.80 +0.1 1,391,019 - Pf6N2E2_1187 0.80 -0.6 1,391,135 + Pf6N2E2_1187 0.89 -0.5 1,391,135 + Pf6N2E2_1187 0.89 -1.3 1,391,171 - +1.1 1,391,234 + +0.9 1,391,239 + +0.1 1,391,239 + -0.1 1,391,273 + +0.1 1,391,273 + -1.6 1,391,274 - +0.2 1,391,276 + +1.2 1,391,276 + +0.8 1,391,276 + +2.1 1,391,276 + +1.4 1,391,277 - +0.4 1,391,277 - -1.5 1,391,360 + +0.2 1,391,496 + Pf6N2E2_1188 0.25 -1.6 1,391,524 + Pf6N2E2_1188 0.28 -2.5 1,391,525 - Pf6N2E2_1188 0.28 -0.2 1,391,525 - Pf6N2E2_1188 0.28 -0.0 1,391,558 - Pf6N2E2_1188 0.32 -1.5 1,391,803 - Pf6N2E2_1188 0.61 -0.6 1,391,863 + Pf6N2E2_1188 0.68 +0.4 1,392,004 + Pf6N2E2_1188 0.84 -1.4 1,392,004 + Pf6N2E2_1188 0.84 -0.9 1,392,005 - Pf6N2E2_1188 0.84 -0.4 1,392,005 - Pf6N2E2_1188 0.84 -1.0 1,392,043 - Pf6N2E2_1188 0.89 +0.5 1,392,281 - Pf6N2E2_1189 0.13 -0.4 1,392,281 - Pf6N2E2_1189 0.13 +0.0 1,392,534 + Pf6N2E2_1189 0.34 +1.2 1,392,535 - Pf6N2E2_1189 0.35 +0.4 1,392,551 - Pf6N2E2_1189 0.36 -1.2 1,392,551 - Pf6N2E2_1189 0.36 +0.1 1,392,683 + Pf6N2E2_1189 0.47 +1.7 1,392,684 - Pf6N2E2_1189 0.47 -1.1 1,392,831 + Pf6N2E2_1189 0.60 +1.4 1,393,047 + Pf6N2E2_1189 0.78 +0.4 1,393,048 - Pf6N2E2_1189 0.78 -0.2 1,393,056 + Pf6N2E2_1189 0.79 +0.8 1,393,057 - Pf6N2E2_1189 0.79 +0.4 1,393,110 + Pf6N2E2_1189 0.84 +0.6
Or see this region's nucleotide sequence