Experiment: Spectinomycin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_114 and Pf6N2E2_115 overlap by 4 nucleotides Pf6N2E2_115 and Pf6N2E2_116 are separated by 12 nucleotides
Pf6N2E2_114 - Polysaccharide biosynthesis protein, at 160,330 to 161,634
_114
Pf6N2E2_115 - FIG00958408: hypothetical protein, at 161,631 to 162,635
_115
Pf6N2E2_116 - Glycosyltransferase, at 162,648 to 163,784
_116
Position (kb) 161
162
163 Strain fitness (log2 ratio) -2
-1
0
1 at 161.390 kb on - strand, within Pf6N2E2_114 at 161.618 kb on + strand at 161.741 kb on - strand, within Pf6N2E2_115 at 161.828 kb on - strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.833 kb on + strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 161.834 kb on - strand, within Pf6N2E2_115 at 162.085 kb on + strand, within Pf6N2E2_115 at 162.086 kb on - strand, within Pf6N2E2_115 at 162.086 kb on - strand at 162.278 kb on + strand, within Pf6N2E2_115 at 162.812 kb on - strand, within Pf6N2E2_116 at 162.844 kb on + strand, within Pf6N2E2_116 at 162.949 kb on + strand, within Pf6N2E2_116 at 163.091 kb on - strand, within Pf6N2E2_116 at 163.091 kb on - strand, within Pf6N2E2_116 at 163.256 kb on - strand, within Pf6N2E2_116 at 163.256 kb on - strand, within Pf6N2E2_116 at 163.579 kb on + strand, within Pf6N2E2_116
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.01 mg/ml remove 161,390 - Pf6N2E2_114 0.81 +0.4 161,618 + -0.4 161,741 - Pf6N2E2_115 0.11 -0.7 161,828 - Pf6N2E2_115 0.20 -0.7 161,833 + Pf6N2E2_115 0.20 +0.8 161,833 + Pf6N2E2_115 0.20 +0.4 161,833 + Pf6N2E2_115 0.20 -1.0 161,833 + Pf6N2E2_115 0.20 -0.0 161,834 - Pf6N2E2_115 0.20 +0.3 161,834 - Pf6N2E2_115 0.20 -1.0 161,834 - Pf6N2E2_115 0.20 +0.9 161,834 - Pf6N2E2_115 0.20 -1.6 161,834 - Pf6N2E2_115 0.20 -1.1 161,834 - Pf6N2E2_115 0.20 -0.5 161,834 - Pf6N2E2_115 0.20 -0.1 161,834 - Pf6N2E2_115 0.20 -0.5 161,834 - Pf6N2E2_115 0.20 -0.1 162,085 + Pf6N2E2_115 0.45 -0.2 162,086 - Pf6N2E2_115 0.45 +0.7 162,086 - +0.7 162,278 + Pf6N2E2_115 0.64 -1.8 162,812 - Pf6N2E2_116 0.14 -0.0 162,844 + Pf6N2E2_116 0.17 +0.9 162,949 + Pf6N2E2_116 0.26 -1.9 163,091 - Pf6N2E2_116 0.39 -0.5 163,091 - Pf6N2E2_116 0.39 +0.2 163,256 - Pf6N2E2_116 0.53 -0.3 163,256 - Pf6N2E2_116 0.53 +0.6 163,579 + Pf6N2E2_116 0.82 -0.4
Or see this region's nucleotide sequence