Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23240 and BWI76_RS23245 are separated by 12 nucleotides BWI76_RS23245 and BWI76_RS23250 overlap by 16 nucleotides BWI76_RS23250 and BWI76_RS23255 overlap by 4 nucleotides BWI76_RS23255 and BWI76_RS23260 overlap by 10 nucleotides
BWI76_RS23240: BWI76_RS23240 - exonuclease V subunit gamma, at 4,846,841 to 4,850,215
_RS23240
BWI76_RS23245: BWI76_RS23245 - hypothetical protein, at 4,850,228 to 4,850,551
_RS23245
BWI76_RS23250: BWI76_RS23250 - hypothetical protein, at 4,850,536 to 4,850,943
_RS23250
BWI76_RS23255: BWI76_RS23255 - prepilin-type cleavage/methylation domain-containing protein, at 4,850,940 to 4,851,503
_RS23255
BWI76_RS23260: BWI76_RS23260 - prepilin-type cleavage/methylation domain-containing protein, at 4,851,494 to 4,851,961
_RS23260
Position (kb) 4850
4851 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4849.704 kb on + strand, within BWI76_RS23240 at 4849.712 kb on - strand, within BWI76_RS23240 at 4850.375 kb on - strand, within BWI76_RS23245 at 4850.433 kb on + strand, within BWI76_RS23245 at 4850.679 kb on - strand, within BWI76_RS23250 at 4850.784 kb on - strand, within BWI76_RS23250 at 4850.953 kb on - strand at 4851.173 kb on + strand, within BWI76_RS23255 at 4851.397 kb on - strand, within BWI76_RS23255 at 4851.397 kb on - strand, within BWI76_RS23255 at 4851.399 kb on + strand, within BWI76_RS23255 at 4851.499 kb on + strand at 4851.617 kb on - strand, within BWI76_RS23260 at 4851.617 kb on - strand at 4851.642 kb on - strand, within BWI76_RS23260 at 4851.803 kb on + strand, within BWI76_RS23260
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,849,704 + BWI76_RS23240 0.85 -2.6 4,849,712 - BWI76_RS23240 0.85 -2.8 4,850,375 - BWI76_RS23245 0.45 -1.1 4,850,433 + BWI76_RS23245 0.63 -0.2 4,850,679 - BWI76_RS23250 0.35 -0.0 4,850,784 - BWI76_RS23250 0.61 -0.9 4,850,953 - -0.1 4,851,173 + BWI76_RS23255 0.41 -0.7 4,851,397 - BWI76_RS23255 0.81 +0.2 4,851,397 - BWI76_RS23255 0.81 +1.1 4,851,399 + BWI76_RS23255 0.81 -0.9 4,851,499 + +1.3 4,851,617 - BWI76_RS23260 0.26 +0.1 4,851,617 - +2.1 4,851,642 - BWI76_RS23260 0.32 +0.1 4,851,803 + BWI76_RS23260 0.66 +0.8
Or see this region's nucleotide sequence