Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13175

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13165 and BWI76_RS13170 are separated by 48 nucleotidesBWI76_RS13170 and BWI76_RS13175 are separated by 15 nucleotidesBWI76_RS13175 and BWI76_RS13180 overlap by 4 nucleotides BWI76_RS13165: BWI76_RS13165 - ATP-dependent RNA helicase HrpA, at 2,744,380 to 2,748,282 _RS13165 BWI76_RS13170: BWI76_RS13170 - methyltransferase, at 2,748,331 to 2,748,990 _RS13170 BWI76_RS13175: BWI76_RS13175 - NodT family RND efflux system outer membrane lipoprotein, at 2,749,006 to 2,750,385 _RS13175 BWI76_RS13180: BWI76_RS13180 - multidrug transporter EmrA, at 2,750,382 to 2,751,467 _RS13180 Position (kb) 2749 2750 2751Strain fitness (log2 ratio) -1 0 1at 2748.216 kb on + strandat 2748.513 kb on + strand, within BWI76_RS13170at 2748.726 kb on + strand, within BWI76_RS13170at 2748.768 kb on - strand, within BWI76_RS13170at 2749.948 kb on + strand, within BWI76_RS13175at 2750.018 kb on + strand, within BWI76_RS13175at 2750.018 kb on + strand, within BWI76_RS13175at 2750.911 kb on + strand, within BWI76_RS13180at 2750.945 kb on - strand, within BWI76_RS13180at 2751.173 kb on + strand, within BWI76_RS13180at 2751.181 kb on - strand, within BWI76_RS13180

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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2,748,216 + +0.8
2,748,513 + BWI76_RS13170 0.28 -0.3
2,748,726 + BWI76_RS13170 0.60 -0.0
2,748,768 - BWI76_RS13170 0.66 +0.3
2,749,948 + BWI76_RS13175 0.68 +0.7
2,750,018 + BWI76_RS13175 0.73 +0.6
2,750,018 + BWI76_RS13175 0.73 +0.2
2,750,911 + BWI76_RS13180 0.49 -0.3
2,750,945 - BWI76_RS13180 0.52 -1.4
2,751,173 + BWI76_RS13180 0.73 -0.4
2,751,181 - BWI76_RS13180 0.74 +0.1

Or see this region's nucleotide sequence