Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13125

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13115 and BWI76_RS13120 are separated by 2 nucleotidesBWI76_RS13120 and BWI76_RS13125 are separated by 3 nucleotidesBWI76_RS13125 and BWI76_RS13130 overlap by 11 nucleotidesBWI76_RS13130 and BWI76_RS13135 overlap by 1 nucleotides BWI76_RS13115: BWI76_RS13115 - 2,3-dehydroadipyl-CoA hydratase, at 2,735,366 to 2,736,133 _RS13115 BWI76_RS13120: BWI76_RS13120 - 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase, at 2,736,136 to 2,736,924 _RS13120 BWI76_RS13125: BWI76_RS13125 - 3-hydroxyacyl-CoA dehydrogenase, at 2,736,928 to 2,738,349 _RS13125 BWI76_RS13130: BWI76_RS13130 - phenylacetic acid degradation protein PaaD, at 2,738,339 to 2,738,761 _RS13130 BWI76_RS13135: BWI76_RS13135 - 3-oxoadipyl-CoA thiolase, at 2,738,761 to 2,739,966 _RS13135 Position (kb) 2736 2737 2738 2739Strain fitness (log2 ratio) -1 0 1at 2736.150 kb on - strandat 2736.166 kb on - strandat 2736.758 kb on + strand, within BWI76_RS13120at 2736.963 kb on - strandat 2737.662 kb on + strand, within BWI76_RS13125at 2737.786 kb on + strand, within BWI76_RS13125at 2737.794 kb on - strand, within BWI76_RS13125at 2737.877 kb on + strand, within BWI76_RS13125at 2738.546 kb on + strand, within BWI76_RS13130at 2738.845 kb on - strandat 2738.935 kb on - strand, within BWI76_RS13135at 2739.311 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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2,736,150 - -0.3
2,736,166 - -0.5
2,736,758 + BWI76_RS13120 0.79 -0.1
2,736,963 - -0.5
2,737,662 + BWI76_RS13125 0.52 +0.5
2,737,786 + BWI76_RS13125 0.60 +0.3
2,737,794 - BWI76_RS13125 0.61 +0.1
2,737,877 + BWI76_RS13125 0.67 +0.0
2,738,546 + BWI76_RS13130 0.49 +0.1
2,738,845 - +0.2
2,738,935 - BWI76_RS13135 0.14 +0.8
2,739,311 - -1.1

Or see this region's nucleotide sequence