Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS07750

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS07745 and BWI76_RS07750 are separated by 9 nucleotidesBWI76_RS07750 and BWI76_RS07755 are separated by 13 nucleotidesBWI76_RS07755 and BWI76_RS07760 overlap by 1 nucleotides BWI76_RS07745: BWI76_RS07745 - isochorismate synthase EntC, at 1,643,316 to 1,644,491 _RS07745 BWI76_RS07750: BWI76_RS07750 - 2,3-dihydroxybenzoate-AMP ligase, at 1,644,501 to 1,646,108 _RS07750 BWI76_RS07755: BWI76_RS07755 - isochorismatase, at 1,646,122 to 1,646,973 _RS07755 BWI76_RS07760: BWI76_RS07760 - 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase, at 1,646,973 to 1,647,728 _RS07760 Position (kb) 1644 1645 1646 1647Strain fitness (log2 ratio) -2 -1 0 1 2at 1643.579 kb on + strand, within BWI76_RS07745at 1643.587 kb on - strand, within BWI76_RS07745at 1643.596 kb on + strand, within BWI76_RS07745at 1643.670 kb on - strand, within BWI76_RS07745at 1643.848 kb on + strand, within BWI76_RS07745at 1643.848 kb on + strand, within BWI76_RS07745at 1644.263 kb on - strand, within BWI76_RS07745at 1644.795 kb on - strand, within BWI76_RS07750at 1644.809 kb on + strand, within BWI76_RS07750at 1644.844 kb on - strand, within BWI76_RS07750at 1645.289 kb on - strand, within BWI76_RS07750at 1645.853 kb on + strand, within BWI76_RS07750at 1645.886 kb on - strand, within BWI76_RS07750at 1645.886 kb on - strand, within BWI76_RS07750at 1645.888 kb on + strand, within BWI76_RS07750at 1645.929 kb on - strand, within BWI76_RS07750at 1646.004 kb on + strandat 1646.103 kb on + strandat 1646.110 kb on + strandat 1646.283 kb on - strand, within BWI76_RS07755at 1646.308 kb on - strand, within BWI76_RS07755at 1646.351 kb on - strand, within BWI76_RS07755at 1646.351 kb on - strand, within BWI76_RS07755at 1647.053 kb on - strand, within BWI76_RS07760at 1647.090 kb on - strand, within BWI76_RS07760

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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1,643,579 + BWI76_RS07745 0.22 +0.4
1,643,587 - BWI76_RS07745 0.23 -0.1
1,643,596 + BWI76_RS07745 0.24 -0.8
1,643,670 - BWI76_RS07745 0.30 -0.1
1,643,848 + BWI76_RS07745 0.45 -0.1
1,643,848 + BWI76_RS07745 0.45 -0.3
1,644,263 - BWI76_RS07745 0.81 -0.3
1,644,795 - BWI76_RS07750 0.18 +2.4
1,644,809 + BWI76_RS07750 0.19 +0.4
1,644,844 - BWI76_RS07750 0.21 +0.6
1,645,289 - BWI76_RS07750 0.49 +0.7
1,645,853 + BWI76_RS07750 0.84 +0.2
1,645,886 - BWI76_RS07750 0.86 +0.5
1,645,886 - BWI76_RS07750 0.86 -0.0
1,645,888 + BWI76_RS07750 0.86 +0.6
1,645,929 - BWI76_RS07750 0.89 +0.1
1,646,004 + -0.1
1,646,103 + -0.0
1,646,110 + -2.7
1,646,283 - BWI76_RS07755 0.19 -0.5
1,646,308 - BWI76_RS07755 0.22 -0.1
1,646,351 - BWI76_RS07755 0.27 +0.2
1,646,351 - BWI76_RS07755 0.27 +0.6
1,647,053 - BWI76_RS07760 0.11 +0.5
1,647,090 - BWI76_RS07760 0.15 -0.8

Or see this region's nucleotide sequence