Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04940 and MMP_RS04945 are separated by 290 nucleotides MMP_RS04945 and MMP_RS04950 are separated by 559 nucleotides
MMP_RS04940: MMP_RS04940 - precorrin-3B C(17)-methyltransferase, at 940,779 to 941,531
_RS04940
MMP_RS04945: MMP_RS04945 - hypothetical protein, at 941,822 to 942,295
_RS04945
MMP_RS04950: MMP_RS04950 - succinate--CoA ligase subunit alpha, at 942,855 to 943,718
_RS04950
Position (kb) 941
942
943 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 940.839 kb on + strand at 940.865 kb on - strand at 940.886 kb on - strand at 940.897 kb on + strand at 940.901 kb on - strand at 941.003 kb on + strand at 941.188 kb on + strand at 941.188 kb on + strand at 941.188 kb on + strand at 941.223 kb on - strand at 941.290 kb on - strand at 941.290 kb on - strand at 941.290 kb on - strand at 941.291 kb on + strand at 941.292 kb on - strand at 941.292 kb on - strand at 941.292 kb on - strand at 941.293 kb on + strand at 941.293 kb on + strand at 941.293 kb on + strand at 941.293 kb on + strand at 941.293 kb on + strand at 941.293 kb on + strand at 941.346 kb on - strand at 941.376 kb on + strand at 941.419 kb on - strand at 941.420 kb on + strand at 941.448 kb on - strand at 941.457 kb on + strand at 941.524 kb on - strand at 941.524 kb on - strand at 941.524 kb on - strand at 941.554 kb on - strand at 941.580 kb on - strand at 941.584 kb on - strand at 941.590 kb on - strand at 941.591 kb on + strand at 941.591 kb on + strand at 941.591 kb on + strand at 941.592 kb on - strand at 941.593 kb on + strand at 941.598 kb on - strand at 941.598 kb on - strand at 941.599 kb on + strand at 941.606 kb on + strand at 941.618 kb on - strand at 941.619 kb on + strand at 941.619 kb on + strand at 941.662 kb on + strand at 941.686 kb on - strand at 941.687 kb on + strand at 941.687 kb on + strand at 941.694 kb on - strand at 941.695 kb on + strand at 941.697 kb on - strand at 941.697 kb on - strand at 941.705 kb on - strand at 941.724 kb on - strand at 941.741 kb on - strand at 941.742 kb on + strand at 941.744 kb on - strand at 941.745 kb on + strand at 941.745 kb on + strand at 941.745 kb on + strand at 941.745 kb on + strand at 941.748 kb on + strand at 941.748 kb on + strand at 941.757 kb on - strand at 941.757 kb on - strand at 941.758 kb on + strand at 941.759 kb on - strand at 941.759 kb on - strand at 941.759 kb on - strand at 941.772 kb on - strand at 941.772 kb on - strand at 941.773 kb on + strand at 941.773 kb on + strand at 941.773 kb on + strand at 941.773 kb on + strand at 941.774 kb on - strand at 941.775 kb on + strand at 941.777 kb on + strand at 941.835 kb on + strand at 941.881 kb on - strand, within MMP_RS04945 at 941.885 kb on - strand, within MMP_RS04945 at 942.062 kb on - strand, within MMP_RS04945 at 942.071 kb on - strand, within MMP_RS04945 at 942.172 kb on + strand, within MMP_RS04945 at 942.186 kb on - strand, within MMP_RS04945 at 942.276 kb on + strand at 942.325 kb on - strand at 942.335 kb on - strand at 942.340 kb on + strand at 942.340 kb on + strand at 942.343 kb on + strand at 942.352 kb on + strand at 942.360 kb on - strand at 942.360 kb on - strand at 942.364 kb on - strand at 942.365 kb on + strand at 942.368 kb on + strand at 942.368 kb on + strand at 942.368 kb on + strand at 942.368 kb on + strand at 942.377 kb on + strand at 942.377 kb on + strand at 942.380 kb on - strand at 942.380 kb on - strand at 942.381 kb on + strand at 942.381 kb on + strand at 942.382 kb on - strand at 942.383 kb on + strand at 942.398 kb on - strand at 942.399 kb on + strand at 942.403 kb on + strand at 942.408 kb on + strand at 942.409 kb on - strand at 942.432 kb on - strand at 942.432 kb on - strand at 942.445 kb on + strand at 942.451 kb on - strand at 942.456 kb on - strand at 942.458 kb on - strand at 942.458 kb on - strand at 942.459 kb on + strand at 942.459 kb on + strand at 942.459 kb on + strand at 942.460 kb on - strand at 942.461 kb on + strand at 942.470 kb on + strand at 942.491 kb on - strand at 942.492 kb on + strand at 942.492 kb on + strand at 942.496 kb on + strand at 942.500 kb on + strand at 942.502 kb on + strand at 942.504 kb on - strand at 942.504 kb on - strand at 942.504 kb on - strand at 942.505 kb on + strand at 942.505 kb on + strand at 942.505 kb on + strand at 942.505 kb on + strand at 942.510 kb on + strand at 942.514 kb on - strand at 942.520 kb on + strand at 942.520 kb on + strand at 942.521 kb on - strand at 942.522 kb on + strand at 942.522 kb on + strand at 942.525 kb on - strand at 942.526 kb on + strand at 942.526 kb on + strand at 942.526 kb on + strand at 942.553 kb on + strand at 942.568 kb on - strand at 942.568 kb on - strand at 942.569 kb on + strand at 942.569 kb on + strand at 942.569 kb on + strand at 942.576 kb on - strand at 942.576 kb on - strand at 942.576 kb on - strand at 942.576 kb on - strand at 942.577 kb on + strand at 942.577 kb on + strand at 942.578 kb on - strand at 942.579 kb on + strand at 942.582 kb on - strand at 942.582 kb on - strand at 942.582 kb on - strand at 942.582 kb on - strand at 942.582 kb on - strand at 942.583 kb on + strand at 942.595 kb on - strand at 942.595 kb on - strand at 942.595 kb on - strand at 942.595 kb on - strand at 942.596 kb on + strand at 942.598 kb on + strand at 942.612 kb on + strand at 942.620 kb on - strand at 942.653 kb on - strand at 942.653 kb on - strand at 942.653 kb on - strand at 942.654 kb on + strand at 942.654 kb on + strand at 942.654 kb on + strand at 942.654 kb on + strand at 942.654 kb on + strand at 942.654 kb on + strand at 942.657 kb on - strand at 942.657 kb on - strand at 942.658 kb on + strand at 942.658 kb on + strand at 942.658 kb on + strand at 942.661 kb on - strand at 942.663 kb on - strand at 942.663 kb on - strand at 942.664 kb on + strand at 942.688 kb on - strand at 942.726 kb on - strand at 942.752 kb on - strand at 942.772 kb on - strand at 942.773 kb on + strand at 942.775 kb on + strand at 942.776 kb on - strand at 942.776 kb on - strand at 942.777 kb on + strand at 942.787 kb on + strand at 942.825 kb on + strand at 942.841 kb on + strand at 943.069 kb on - strand at 943.099 kb on + strand at 943.106 kb on - strand at 943.277 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 940,839 + -3.0 940,865 - -2.3 940,886 - -4.0 940,897 + -1.5 940,901 - -2.9 941,003 + -3.5 941,188 + -3.3 941,188 + -2.8 941,188 + -2.8 941,223 - -2.1 941,290 - -3.0 941,290 - -3.1 941,290 - -2.0 941,291 + -3.3 941,292 - -2.7 941,292 - -1.9 941,292 - -4.3 941,293 + -4.1 941,293 + -3.1 941,293 + -1.7 941,293 + -3.8 941,293 + -2.4 941,293 + -3.4 941,346 - -2.4 941,376 + -4.5 941,419 - -2.3 941,420 + -4.2 941,448 - -2.7 941,457 + -2.9 941,524 - -3.1 941,524 - -3.4 941,524 - -2.9 941,554 - -1.9 941,580 - -0.9 941,584 - -3.0 941,590 - -2.3 941,591 + -2.1 941,591 + -3.6 941,591 + -1.0 941,592 - -2.2 941,593 + -5.4 941,598 - -0.1 941,598 - -1.6 941,599 + -0.4 941,606 + -0.9 941,618 - -0.0 941,619 + +0.1 941,619 + -0.3 941,662 + -1.0 941,686 - -0.3 941,687 + -0.2 941,687 + -0.6 941,694 - -0.4 941,695 + -0.3 941,697 - -1.2 941,697 - -0.4 941,705 - -0.3 941,724 - +0.6 941,741 - -0.3 941,742 + -0.4 941,744 - +0.1 941,745 + -0.4 941,745 + +0.2 941,745 + -0.5 941,745 + +0.1 941,748 + +0.2 941,748 + -0.4 941,757 - +0.3 941,757 - +1.7 941,758 + -0.4 941,759 - -0.3 941,759 - +1.1 941,759 - +0.2 941,772 - +0.6 941,772 - +0.4 941,773 + +0.4 941,773 + +0.7 941,773 + -0.0 941,773 + +0.1 941,774 - -0.3 941,775 + -0.9 941,777 + +0.1 941,835 + +0.1 941,881 - MMP_RS04945 0.12 +1.6 941,885 - MMP_RS04945 0.13 +3.4 942,062 - MMP_RS04945 0.51 +2.4 942,071 - MMP_RS04945 0.53 +1.8 942,172 + MMP_RS04945 0.74 +2.1 942,186 - MMP_RS04945 0.77 +2.4 942,276 + +1.6 942,325 - +1.0 942,335 - -0.6 942,340 + +1.7 942,340 + +2.5 942,343 + -0.4 942,352 + +2.3 942,360 - +1.3 942,360 - +0.9 942,364 - +0.0 942,365 + +1.3 942,368 + +0.8 942,368 + +2.3 942,368 + +0.8 942,368 + +1.9 942,377 + +0.4 942,377 + +0.6 942,380 - +0.1 942,380 - -1.3 942,381 + +1.0 942,381 + +0.7 942,382 - -0.4 942,383 + +0.2 942,398 - -0.1 942,399 + -0.2 942,403 + +1.3 942,408 + +1.2 942,409 - +0.3 942,432 - -0.0 942,432 - -0.4 942,445 + +0.1 942,451 - -0.4 942,456 - -0.4 942,458 - +0.5 942,458 - +0.4 942,459 + +0.4 942,459 + +0.4 942,459 + +0.5 942,460 - -0.3 942,461 + -0.8 942,470 + +1.2 942,491 - +0.2 942,492 + +0.0 942,492 + +0.1 942,496 + +0.0 942,500 + -0.5 942,502 + -0.2 942,504 - +0.5 942,504 - +0.5 942,504 - +0.5 942,505 + +0.1 942,505 + -0.2 942,505 + +0.1 942,505 + +0.7 942,510 + -0.7 942,514 - +0.5 942,520 + +0.3 942,520 + +1.0 942,521 - +0.3 942,522 + +0.1 942,522 + +0.4 942,525 - -0.1 942,526 + -0.5 942,526 + +0.1 942,526 + +0.1 942,553 + +0.6 942,568 - -0.0 942,568 - +0.4 942,569 + +0.1 942,569 + +0.7 942,569 + -0.3 942,576 - -1.0 942,576 - -0.2 942,576 - -0.7 942,576 - +0.1 942,577 + -0.0 942,577 + +0.3 942,578 - +0.1 942,579 + +0.4 942,582 - +0.0 942,582 - -0.1 942,582 - -0.4 942,582 - +0.6 942,582 - -0.1 942,583 + +0.0 942,595 - +0.3 942,595 - +0.3 942,595 - +0.3 942,595 - +0.3 942,596 + -0.3 942,598 + +0.3 942,612 + +1.0 942,620 - -0.9 942,653 - -0.3 942,653 - +0.4 942,653 - -0.4 942,654 + +0.2 942,654 + -1.0 942,654 + -0.1 942,654 + +0.1 942,654 + -0.0 942,654 + +0.4 942,657 - -0.3 942,657 - +0.3 942,658 + +0.3 942,658 + +1.1 942,658 + +0.4 942,661 - +0.5 942,663 - +0.3 942,663 - +0.3 942,664 + -0.7 942,688 - -0.0 942,726 - -1.2 942,752 - -0.9 942,772 - -1.2 942,773 + +0.8 942,775 + -3.0 942,776 - -2.9 942,776 - -3.8 942,777 + -1.6 942,787 + -2.5 942,825 + -2.1 942,841 + -0.9 943,069 - -3.2 943,099 + -2.5 943,106 - -2.6 943,277 + -0.5
Or see this region's nucleotide sequence