Experiment: H2 N-free with 2g/L casaminos 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03300 and MMP_RS03305 are separated by 168 nucleotides MMP_RS03305 and MMP_RS03310 are separated by 177 nucleotides MMP_RS03310 and MMP_RS08960 are separated by 14 nucleotides MMP_RS08960 and MMP_RS03315 are separated by 114 nucleotides MMP_RS03315 and MMP_RS03320 are separated by 111 nucleotides
MMP_RS03300: MMP_RS03300 - TOBE domain-containing protein, at 620,371 to 620,577
_RS03300
MMP_RS03305: MMP_RS03305 - winged helix-turn-helix domain-containing protein, at 620,746 to 621,144
_RS03305
MMP_RS03310: MMP_RS03310 - ferrous iron transport protein B, at 621,322 to 623,265
_RS03310
MMP_RS08960: MMP_RS08960 - hypothetical protein, at 623,280 to 623,417
_RS08960
MMP_RS03315: MMP_RS03315 - ferrous iron transport protein A, at 623,532 to 623,738
_RS03315
MMP_RS03320: MMP_RS03320 - metal-dependent transcriptional regulator, at 623,850 to 624,254
_RS03320
Position (kb) 621
622
623
624 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 620.336 kb on - strand at 620.368 kb on - strand at 620.369 kb on + strand at 620.369 kb on + strand at 620.370 kb on - strand at 620.382 kb on - strand at 620.546 kb on - strand, within MMP_RS03300 at 620.642 kb on - strand at 620.643 kb on + strand at 620.645 kb on + strand at 620.662 kb on + strand at 620.664 kb on + strand at 620.679 kb on + strand at 620.682 kb on - strand at 620.682 kb on - strand at 620.683 kb on + strand at 620.685 kb on + strand at 620.685 kb on + strand at 620.686 kb on - strand at 620.692 kb on - strand at 620.716 kb on + strand at 620.793 kb on + strand, within MMP_RS03305 at 620.793 kb on + strand, within MMP_RS03305 at 620.793 kb on + strand, within MMP_RS03305 at 620.793 kb on + strand, within MMP_RS03305 at 620.835 kb on + strand, within MMP_RS03305 at 620.859 kb on - strand, within MMP_RS03305 at 620.859 kb on - strand, within MMP_RS03305 at 620.859 kb on - strand, within MMP_RS03305 at 620.891 kb on - strand, within MMP_RS03305 at 620.930 kb on + strand, within MMP_RS03305 at 621.127 kb on + strand at 621.142 kb on - strand at 621.167 kb on + strand at 621.196 kb on - strand at 621.239 kb on - strand at 621.239 kb on - strand at 621.267 kb on - strand at 621.268 kb on + strand at 621.268 kb on + strand at 621.271 kb on - strand at 621.273 kb on - strand at 621.323 kb on - strand at 621.334 kb on - strand at 621.334 kb on - strand at 621.350 kb on - strand at 621.350 kb on - strand at 621.355 kb on - strand at 621.390 kb on + strand at 621.855 kb on - strand at 621.894 kb on - strand at 622.326 kb on - strand at 622.331 kb on + strand at 622.385 kb on + strand at 622.838 kb on + strand at 623.036 kb on - strand at 623.247 kb on - strand at 623.421 kb on - strand at 623.462 kb on - strand at 623.473 kb on + strand at 623.497 kb on + strand at 623.504 kb on + strand at 623.517 kb on - strand at 623.693 kb on - strand, within MMP_RS03315 at 623.702 kb on - strand, within MMP_RS03315 at 623.704 kb on - strand, within MMP_RS03315 at 623.755 kb on - strand at 623.755 kb on - strand at 623.756 kb on + strand at 623.822 kb on - strand at 623.823 kb on + strand at 623.823 kb on + strand at 623.824 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.827 kb on + strand at 623.828 kb on - strand at 623.828 kb on - strand at 623.828 kb on - strand at 623.887 kb on - strand at 623.897 kb on - strand, within MMP_RS03320 at 623.901 kb on + strand, within MMP_RS03320 at 623.909 kb on - strand, within MMP_RS03320 at 624.012 kb on - strand, within MMP_RS03320 at 624.023 kb on - strand, within MMP_RS03320
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 2g/L casaminos 10mM Ammonium chloride remove 620,336 - +0.2 620,368 - +0.0 620,369 + -0.0 620,369 + +0.9 620,370 - +0.1 620,382 - +0.2 620,546 - MMP_RS03300 0.85 +0.5 620,642 - +0.5 620,643 + -0.0 620,645 + -1.2 620,662 + -0.3 620,664 + +0.5 620,679 + +0.0 620,682 - -0.1 620,682 - +0.4 620,683 + -1.2 620,685 + -0.3 620,685 + -0.8 620,686 - +0.2 620,692 - -0.6 620,716 + -0.3 620,793 + MMP_RS03305 0.12 -0.1 620,793 + MMP_RS03305 0.12 -0.2 620,793 + MMP_RS03305 0.12 -0.6 620,793 + MMP_RS03305 0.12 -0.0 620,835 + MMP_RS03305 0.22 -0.4 620,859 - MMP_RS03305 0.28 -0.1 620,859 - MMP_RS03305 0.28 -0.6 620,859 - MMP_RS03305 0.28 -2.5 620,891 - MMP_RS03305 0.36 -0.6 620,930 + MMP_RS03305 0.46 +0.4 621,127 + -0.2 621,142 - +0.3 621,167 + +0.5 621,196 - +1.7 621,239 - +0.5 621,239 - -0.2 621,267 - +0.4 621,268 + -1.2 621,268 + +0.1 621,271 - -0.0 621,273 - -0.2 621,323 - -0.0 621,334 - -2.4 621,334 - -2.4 621,350 - -3.0 621,350 - -3.3 621,355 - -3.3 621,390 + -2.6 621,855 - -3.1 621,894 - -2.8 622,326 - -0.9 622,331 + -4.3 622,385 + -2.5 622,838 + -2.9 623,036 - -3.1 623,247 - -2.5 623,421 - +0.7 623,462 - +0.3 623,473 + -0.4 623,497 + +0.2 623,504 + +0.1 623,517 - -0.0 623,693 - MMP_RS03315 0.78 -1.5 623,702 - MMP_RS03315 0.82 -3.5 623,704 - MMP_RS03315 0.83 -4.3 623,755 - -1.9 623,755 - -2.9 623,756 + -2.9 623,822 - -2.4 623,823 + -3.2 623,823 + -2.5 623,824 - -1.9 623,826 - +0.3 623,826 - +0.6 623,826 - -2.0 623,826 - -0.2 623,826 - -1.1 623,826 - +0.5 623,827 + -0.3 623,828 - -1.2 623,828 - +0.3 623,828 - +0.4 623,887 - -0.1 623,897 - MMP_RS03320 0.12 -1.8 623,901 + MMP_RS03320 0.13 -0.9 623,909 - MMP_RS03320 0.15 -0.1 624,012 - MMP_RS03320 0.40 -1.7 624,023 - MMP_RS03320 0.43 -0.9
Or see this region's nucleotide sequence