Experiment: Chloride 1000 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02510 and BWI76_RS02515 overlap by 8 nucleotides BWI76_RS02515 and BWI76_RS02520 are separated by 16 nucleotides BWI76_RS02520 and BWI76_RS02525 are separated by 10 nucleotides
BWI76_RS02510: BWI76_RS02510 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 513,681 to 515,207
_RS02510
BWI76_RS02515: BWI76_RS02515 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 515,200 to 515,661
_RS02515
BWI76_RS02520: BWI76_RS02520 - N-acetylmuramoyl-L-alanine amidase, at 515,678 to 517,015
_RS02520
BWI76_RS02525: BWI76_RS02525 - DNA mismatch repair protein MutL, at 517,026 to 518,921
_RS02525
Position (kb) 515
516
517
518 Strain fitness (log2 ratio) -2
-1
0
1 at 514.698 kb on - strand, within BWI76_RS02510 at 515.007 kb on + strand, within BWI76_RS02510 at 515.054 kb on + strand, within BWI76_RS02510 at 515.689 kb on - strand at 515.816 kb on + strand, within BWI76_RS02520 at 515.890 kb on - strand, within BWI76_RS02520 at 515.890 kb on - strand, within BWI76_RS02520 at 515.890 kb on - strand, within BWI76_RS02520 at 516.024 kb on + strand, within BWI76_RS02520 at 516.171 kb on - strand, within BWI76_RS02520 at 516.196 kb on + strand, within BWI76_RS02520 at 516.204 kb on - strand, within BWI76_RS02520 at 516.367 kb on + strand, within BWI76_RS02520 at 516.573 kb on + strand, within BWI76_RS02520 at 516.610 kb on - strand, within BWI76_RS02520 at 516.702 kb on + strand, within BWI76_RS02520 at 516.739 kb on + strand, within BWI76_RS02520 at 516.810 kb on + strand, within BWI76_RS02520 at 517.279 kb on + strand, within BWI76_RS02525 at 517.279 kb on + strand, within BWI76_RS02525 at 517.287 kb on - strand, within BWI76_RS02525 at 517.317 kb on + strand, within BWI76_RS02525 at 517.317 kb on + strand, within BWI76_RS02525 at 517.325 kb on - strand, within BWI76_RS02525 at 517.358 kb on - strand, within BWI76_RS02525 at 517.464 kb on - strand, within BWI76_RS02525 at 517.790 kb on + strand, within BWI76_RS02525 at 517.893 kb on - strand, within BWI76_RS02525 at 517.926 kb on - strand, within BWI76_RS02525
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 1000 mM remove 514,698 - BWI76_RS02510 0.67 -1.1 515,007 + BWI76_RS02510 0.87 +0.1 515,054 + BWI76_RS02510 0.90 +0.3 515,689 - -0.2 515,816 + BWI76_RS02520 0.10 -0.8 515,890 - BWI76_RS02520 0.16 -0.5 515,890 - BWI76_RS02520 0.16 +0.4 515,890 - BWI76_RS02520 0.16 -0.8 516,024 + BWI76_RS02520 0.26 -0.3 516,171 - BWI76_RS02520 0.37 -2.0 516,196 + BWI76_RS02520 0.39 -0.9 516,204 - BWI76_RS02520 0.39 -1.0 516,367 + BWI76_RS02520 0.51 -0.5 516,573 + BWI76_RS02520 0.67 -1.1 516,610 - BWI76_RS02520 0.70 -0.7 516,702 + BWI76_RS02520 0.77 -0.4 516,739 + BWI76_RS02520 0.79 -2.6 516,810 + BWI76_RS02520 0.85 -0.3 517,279 + BWI76_RS02525 0.13 -0.2 517,279 + BWI76_RS02525 0.13 -2.6 517,287 - BWI76_RS02525 0.14 +0.2 517,317 + BWI76_RS02525 0.15 +0.0 517,317 + BWI76_RS02525 0.15 -0.4 517,325 - BWI76_RS02525 0.16 +0.5 517,358 - BWI76_RS02525 0.18 -0.1 517,464 - BWI76_RS02525 0.23 -0.5 517,790 + BWI76_RS02525 0.40 -0.7 517,893 - BWI76_RS02525 0.46 -0.8 517,926 - BWI76_RS02525 0.47 -0.6
Or see this region's nucleotide sequence