Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_623 and Pf6N2E2_624 overlap by 4 nucleotides Pf6N2E2_624 and Pf6N2E2_625 are separated by 9 nucleotides Pf6N2E2_625 and Pf6N2E2_626 are separated by 601 nucleotides
Pf6N2E2_623 - FIG00954674: hypothetical protein, at 710,068 to 710,562
_623
Pf6N2E2_624 - hypothetical protein PA3071, at 710,559 to 711,503
_624
Pf6N2E2_625 - MoxR-like ATPase in aerotolerance operon, at 711,513 to 712,472
_625
Pf6N2E2_626 - hypothetical protein, at 713,074 to 713,442
_626
Position (kb) 711
712
713 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 710.628 kb on - strand at 711.067 kb on + strand, within Pf6N2E2_624 at 711.111 kb on - strand, within Pf6N2E2_624 at 711.132 kb on + strand, within Pf6N2E2_624 at 711.132 kb on + strand, within Pf6N2E2_624 at 711.132 kb on + strand, within Pf6N2E2_624 at 711.133 kb on - strand, within Pf6N2E2_624 at 711.303 kb on + strand, within Pf6N2E2_624 at 711.303 kb on + strand, within Pf6N2E2_624 at 711.303 kb on + strand, within Pf6N2E2_624 at 711.303 kb on + strand, within Pf6N2E2_624 at 711.304 kb on - strand, within Pf6N2E2_624 at 711.304 kb on - strand, within Pf6N2E2_624 at 711.497 kb on + strand at 711.498 kb on - strand at 711.635 kb on + strand, within Pf6N2E2_625 at 711.727 kb on + strand, within Pf6N2E2_625 at 711.799 kb on + strand, within Pf6N2E2_625 at 711.823 kb on + strand, within Pf6N2E2_625 at 711.823 kb on + strand, within Pf6N2E2_625 at 711.823 kb on + strand, within Pf6N2E2_625 at 711.823 kb on + strand, within Pf6N2E2_625 at 711.824 kb on - strand, within Pf6N2E2_625 at 712.072 kb on + strand, within Pf6N2E2_625 at 712.073 kb on - strand, within Pf6N2E2_625 at 712.073 kb on - strand, within Pf6N2E2_625 at 712.073 kb on - strand, within Pf6N2E2_625 at 712.073 kb on - strand, within Pf6N2E2_625 at 712.073 kb on - strand, within Pf6N2E2_625 at 712.534 kb on + strand at 712.534 kb on + strand at 712.540 kb on - strand at 712.641 kb on + strand at 712.738 kb on + strand at 712.739 kb on - strand at 712.739 kb on - strand at 712.748 kb on + strand at 712.748 kb on + strand at 712.749 kb on - strand at 712.749 kb on - strand at 712.749 kb on - strand at 712.791 kb on + strand at 712.792 kb on - strand at 712.797 kb on + strand at 712.797 kb on + strand at 712.798 kb on - strand at 712.839 kb on + strand at 712.839 kb on + strand at 713.440 kb on + strand at 713.440 kb on + strand at 713.440 kb on + strand at 713.441 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 710,628 - -2.6 711,067 + Pf6N2E2_624 0.54 -2.9 711,111 - Pf6N2E2_624 0.58 -3.2 711,132 + Pf6N2E2_624 0.61 -1.6 711,132 + Pf6N2E2_624 0.61 -1.5 711,132 + Pf6N2E2_624 0.61 -2.1 711,133 - Pf6N2E2_624 0.61 -2.5 711,303 + Pf6N2E2_624 0.79 -0.6 711,303 + Pf6N2E2_624 0.79 -2.2 711,303 + Pf6N2E2_624 0.79 -2.5 711,303 + Pf6N2E2_624 0.79 -1.9 711,304 - Pf6N2E2_624 0.79 -1.8 711,304 - Pf6N2E2_624 0.79 -1.1 711,497 + -1.7 711,498 - -1.0 711,635 + Pf6N2E2_625 0.13 -3.4 711,727 + Pf6N2E2_625 0.22 -1.2 711,799 + Pf6N2E2_625 0.30 -3.4 711,823 + Pf6N2E2_625 0.32 -0.6 711,823 + Pf6N2E2_625 0.32 -2.4 711,823 + Pf6N2E2_625 0.32 -1.2 711,823 + Pf6N2E2_625 0.32 -1.4 711,824 - Pf6N2E2_625 0.32 -0.8 712,072 + Pf6N2E2_625 0.58 -0.6 712,073 - Pf6N2E2_625 0.58 +0.1 712,073 - Pf6N2E2_625 0.58 -2.7 712,073 - Pf6N2E2_625 0.58 -2.7 712,073 - Pf6N2E2_625 0.58 -3.2 712,073 - Pf6N2E2_625 0.58 -0.9 712,534 + -4.1 712,534 + -0.8 712,540 - +0.3 712,641 + -0.7 712,738 + -1.0 712,739 - -0.2 712,739 - +0.1 712,748 + -2.2 712,748 + -0.4 712,749 - +1.4 712,749 - +1.4 712,749 - +2.0 712,791 + -0.5 712,792 - +1.1 712,797 + +1.3 712,797 + -0.0 712,798 - -2.4 712,839 + -1.3 712,839 + +0.0 713,440 + +1.3 713,440 + -0.4 713,440 + +1.8 713,441 - -1.2
Or see this region's nucleotide sequence