Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_471 and Pf6N2E2_472 are separated by 288 nucleotides Pf6N2E2_472 and Pf6N2E2_473 are separated by 13 nucleotides
Pf6N2E2_471 - putative metalloprotease, at 536,431 to 537,492
_471
Pf6N2E2_472 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 537,781 to 538,122
_472
Pf6N2E2_473 - putative cytochrome p450 oxidoreductase, at 538,136 to 539,272
_473
Position (kb)
537
538
539 Strain fitness (log2 ratio)
-2
-1
0
1 at 536.873 kb on - strand, within Pf6N2E2_471 at 536.942 kb on + strand, within Pf6N2E2_471 at 536.943 kb on - strand, within Pf6N2E2_471 at 537.095 kb on + strand, within Pf6N2E2_471 at 537.096 kb on - strand, within Pf6N2E2_471 at 537.127 kb on - strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.170 kb on + strand, within Pf6N2E2_471 at 537.171 kb on - strand, within Pf6N2E2_471 at 537.171 kb on - strand, within Pf6N2E2_471 at 537.452 kb on + strand at 537.452 kb on + strand at 537.452 kb on + strand at 537.453 kb on - strand at 537.453 kb on - strand at 537.453 kb on - strand at 537.453 kb on - strand at 537.505 kb on - strand at 537.833 kb on + strand, within Pf6N2E2_472 at 537.833 kb on + strand, within Pf6N2E2_472 at 537.833 kb on + strand, within Pf6N2E2_472 at 537.904 kb on + strand, within Pf6N2E2_472 at 537.904 kb on + strand, within Pf6N2E2_472 at 537.905 kb on - strand, within Pf6N2E2_472 at 537.905 kb on - strand, within Pf6N2E2_472 at 538.031 kb on - strand, within Pf6N2E2_472 at 538.212 kb on - strand at 538.617 kb on + strand, within Pf6N2E2_473 at 538.617 kb on + strand, within Pf6N2E2_473 at 538.617 kb on + strand, within Pf6N2E2_473 at 538.617 kb on + strand, within Pf6N2E2_473 at 538.618 kb on - strand, within Pf6N2E2_473 at 538.655 kb on - strand, within Pf6N2E2_473 at 538.655 kb on - strand, within Pf6N2E2_473 at 538.736 kb on + strand, within Pf6N2E2_473
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 536,873 - Pf6N2E2_471 0.42 -0.2 536,942 + Pf6N2E2_471 0.48 +0.6 536,943 - Pf6N2E2_471 0.48 +0.7 537,095 + Pf6N2E2_471 0.63 -0.1 537,096 - Pf6N2E2_471 0.63 -0.4 537,127 - Pf6N2E2_471 0.66 +0.2 537,170 + Pf6N2E2_471 0.70 -0.3 537,170 + Pf6N2E2_471 0.70 -0.4 537,170 + Pf6N2E2_471 0.70 -0.2 537,170 + Pf6N2E2_471 0.70 -0.0 537,171 - Pf6N2E2_471 0.70 -0.4 537,171 - Pf6N2E2_471 0.70 -0.2 537,452 + -0.2 537,452 + +0.0 537,452 + -0.6 537,453 - +1.1 537,453 - -1.2 537,453 - -1.2 537,453 - -0.2 537,505 - -0.8 537,833 + Pf6N2E2_472 0.15 -0.2 537,833 + Pf6N2E2_472 0.15 +0.0 537,833 + Pf6N2E2_472 0.15 -0.2 537,904 + Pf6N2E2_472 0.36 -0.2 537,904 + Pf6N2E2_472 0.36 -0.7 537,905 - Pf6N2E2_472 0.36 -1.5 537,905 - Pf6N2E2_472 0.36 -1.7 538,031 - Pf6N2E2_472 0.73 -1.1 538,212 - +0.5 538,617 + Pf6N2E2_473 0.42 +1.1 538,617 + Pf6N2E2_473 0.42 +0.1 538,617 + Pf6N2E2_473 0.42 -0.2 538,617 + Pf6N2E2_473 0.42 +0.8 538,618 - Pf6N2E2_473 0.42 -0.5 538,655 - Pf6N2E2_473 0.46 +0.2 538,655 - Pf6N2E2_473 0.46 -2.0 538,736 + Pf6N2E2_473 0.53 +0.8
Or see this region's nucleotide sequence