Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_452 and Pf6N2E2_453 are separated by 105 nucleotides Pf6N2E2_453 and Pf6N2E2_454 are separated by 379 nucleotides
Pf6N2E2_452 - Rtn protein, at 513,969 to 515,513
_452
Pf6N2E2_453 - LysR family transcriptional regulator STM2281, at 515,619 to 516,482
_453
Pf6N2E2_454 - Mobile element protein, at 516,862 to 518,130
_454
Position (kb) 515
516
517 Strain fitness (log2 ratio) -2
-1
0
1 at 514.668 kb on + strand, within Pf6N2E2_452 at 514.685 kb on - strand, within Pf6N2E2_452 at 514.685 kb on - strand, within Pf6N2E2_452 at 514.685 kb on - strand, within Pf6N2E2_452 at 514.831 kb on + strand, within Pf6N2E2_452 at 514.831 kb on + strand, within Pf6N2E2_452 at 514.866 kb on + strand, within Pf6N2E2_452 at 514.866 kb on + strand, within Pf6N2E2_452 at 514.867 kb on - strand, within Pf6N2E2_452 at 514.867 kb on - strand, within Pf6N2E2_452 at 515.194 kb on + strand, within Pf6N2E2_452 at 515.195 kb on - strand, within Pf6N2E2_452 at 515.195 kb on - strand, within Pf6N2E2_452 at 515.195 kb on - strand, within Pf6N2E2_452 at 515.195 kb on - strand, within Pf6N2E2_452 at 515.195 kb on - strand, within Pf6N2E2_452 at 515.195 kb on - strand, within Pf6N2E2_452 at 515.213 kb on + strand, within Pf6N2E2_452 at 515.214 kb on - strand, within Pf6N2E2_452 at 515.214 kb on - strand, within Pf6N2E2_452 at 515.214 kb on - strand, within Pf6N2E2_452 at 515.214 kb on - strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand, within Pf6N2E2_452 at 515.304 kb on + strand at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.305 kb on - strand, within Pf6N2E2_452 at 515.413 kb on + strand at 515.413 kb on + strand at 515.414 kb on - strand at 515.442 kb on + strand at 515.569 kb on + strand at 515.569 kb on + strand at 515.569 kb on + strand at 515.569 kb on + strand at 515.569 kb on + strand at 515.569 kb on + strand at 515.570 kb on - strand at 515.570 kb on - strand at 515.972 kb on + strand, within Pf6N2E2_453 at 515.973 kb on - strand, within Pf6N2E2_453 at 516.017 kb on + strand, within Pf6N2E2_453 at 516.508 kb on + strand at 516.606 kb on - strand at 516.606 kb on - strand at 516.610 kb on - strand at 516.610 kb on - strand at 516.610 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 514,668 + Pf6N2E2_452 0.45 +0.0 514,685 - Pf6N2E2_452 0.46 +0.6 514,685 - Pf6N2E2_452 0.46 +0.1 514,685 - Pf6N2E2_452 0.46 +0.1 514,831 + Pf6N2E2_452 0.56 -1.3 514,831 + Pf6N2E2_452 0.56 -2.2 514,866 + Pf6N2E2_452 0.58 +0.2 514,866 + Pf6N2E2_452 0.58 +1.1 514,867 - Pf6N2E2_452 0.58 +0.8 514,867 - Pf6N2E2_452 0.58 +0.8 515,194 + Pf6N2E2_452 0.79 -0.4 515,195 - Pf6N2E2_452 0.79 -0.3 515,195 - Pf6N2E2_452 0.79 +0.6 515,195 - Pf6N2E2_452 0.79 +0.4 515,195 - Pf6N2E2_452 0.79 +0.2 515,195 - Pf6N2E2_452 0.79 +0.1 515,195 - Pf6N2E2_452 0.79 -0.2 515,213 + Pf6N2E2_452 0.81 +0.3 515,214 - Pf6N2E2_452 0.81 -0.1 515,214 - Pf6N2E2_452 0.81 +0.5 515,214 - Pf6N2E2_452 0.81 -1.0 515,214 - Pf6N2E2_452 0.81 -2.2 515,304 + Pf6N2E2_452 0.86 +0.2 515,304 + Pf6N2E2_452 0.86 -2.0 515,304 + Pf6N2E2_452 0.86 -0.7 515,304 + Pf6N2E2_452 0.86 +0.7 515,304 + Pf6N2E2_452 0.86 +1.1 515,304 + Pf6N2E2_452 0.86 +0.7 515,304 + Pf6N2E2_452 0.86 -0.5 515,304 + -0.2 515,305 - Pf6N2E2_452 0.86 +0.6 515,305 - Pf6N2E2_452 0.86 +1.2 515,305 - Pf6N2E2_452 0.86 -0.4 515,305 - Pf6N2E2_452 0.86 +0.9 515,305 - Pf6N2E2_452 0.86 -0.3 515,305 - Pf6N2E2_452 0.86 +1.5 515,305 - Pf6N2E2_452 0.86 +0.1 515,305 - Pf6N2E2_452 0.86 +0.5 515,413 + +1.1 515,413 + -0.7 515,414 - -0.1 515,442 + -1.2 515,569 + -2.1 515,569 + -0.1 515,569 + -0.1 515,569 + -0.1 515,569 + +0.4 515,569 + +0.7 515,570 - +0.9 515,570 - +0.7 515,972 + Pf6N2E2_453 0.41 -1.1 515,973 - Pf6N2E2_453 0.41 -0.5 516,017 + Pf6N2E2_453 0.46 -0.4 516,508 + -0.0 516,606 - -1.7 516,606 - -0.6 516,610 - -0.0 516,610 - -0.6 516,610 - -0.1
Or see this region's nucleotide sequence