Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_415

Experiment: Tetracycline 0.0004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_413 and Pf6N2E2_414 are separated by 2 nucleotidesPf6N2E2_414 and Pf6N2E2_415 are separated by 17 nucleotidesPf6N2E2_415 and Pf6N2E2_416 overlap by 4 nucleotidesPf6N2E2_416 and Pf6N2E2_417 are separated by 39 nucleotides Pf6N2E2_413 - Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61), at 481,369 to 482,442 _413 Pf6N2E2_414 - Chemotaxis protein CheD, at 482,445 to 482,921 _414 Pf6N2E2_415 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 482,939 to 483,775 _415 Pf6N2E2_416 - Positive regulator of CheA protein activity (CheW), at 483,772 to 484,308 _416 Pf6N2E2_417 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 484,348 to 486,006 _417 Position (kb) 482 483 484Strain fitness (log2 ratio) -1 0 1 2at 482.422 kb on + strandat 482.422 kb on + strandat 482.422 kb on + strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.423 kb on - strandat 482.442 kb on + strandat 482.461 kb on - strandat 482.461 kb on - strandat 482.462 kb on + strandat 482.462 kb on + strandat 482.462 kb on + strandat 482.462 kb on + strandat 482.462 kb on + strandat 482.462 kb on + strandat 482.462 kb on + strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.463 kb on - strandat 482.481 kb on + strandat 482.482 kb on - strandat 482.482 kb on - strandat 482.482 kb on - strandat 482.482 kb on - strandat 482.482 kb on - strandat 482.740 kb on + strand, within Pf6N2E2_414at 482.741 kb on - strand, within Pf6N2E2_414at 482.741 kb on - strand, within Pf6N2E2_414at 482.741 kb on - strand, within Pf6N2E2_414at 482.743 kb on - strand, within Pf6N2E2_414at 482.743 kb on - strand, within Pf6N2E2_414at 482.909 kb on - strandat 482.909 kb on - strandat 482.909 kb on - strandat 483.025 kb on - strand, within Pf6N2E2_415at 483.090 kb on + strand, within Pf6N2E2_415at 483.090 kb on + strand, within Pf6N2E2_415at 483.090 kb on + strand, within Pf6N2E2_415at 483.090 kb on + strand, within Pf6N2E2_415at 483.091 kb on - strand, within Pf6N2E2_415at 483.091 kb on - strand, within Pf6N2E2_415at 483.091 kb on - strand, within Pf6N2E2_415at 483.240 kb on + strand, within Pf6N2E2_415at 483.241 kb on - strand, within Pf6N2E2_415at 483.315 kb on - strand, within Pf6N2E2_415at 483.315 kb on - strand, within Pf6N2E2_415at 483.393 kb on + strand, within Pf6N2E2_415at 483.394 kb on - strand, within Pf6N2E2_415at 483.465 kb on - strand, within Pf6N2E2_415at 483.465 kb on - strand, within Pf6N2E2_415at 483.548 kb on + strand, within Pf6N2E2_415at 483.548 kb on + strand, within Pf6N2E2_415at 483.548 kb on + strand, within Pf6N2E2_415at 483.548 kb on + strand, within Pf6N2E2_415at 483.548 kb on + strand, within Pf6N2E2_415at 483.548 kb on + strand, within Pf6N2E2_415at 483.549 kb on - strand, within Pf6N2E2_415at 483.549 kb on - strand, within Pf6N2E2_415at 483.549 kb on - strand, within Pf6N2E2_415at 483.588 kb on + strand, within Pf6N2E2_415at 483.597 kb on + strand, within Pf6N2E2_415at 483.597 kb on + strand, within Pf6N2E2_415at 483.598 kb on - strand, within Pf6N2E2_415at 483.612 kb on + strand, within Pf6N2E2_415at 483.613 kb on - strand, within Pf6N2E2_415at 483.691 kb on - strand, within Pf6N2E2_415at 483.691 kb on - strand, within Pf6N2E2_415at 483.859 kb on + strand, within Pf6N2E2_416at 483.859 kb on + strand, within Pf6N2E2_416at 484.239 kb on + strand, within Pf6N2E2_416at 484.239 kb on + strand, within Pf6N2E2_416at 484.240 kb on - strand, within Pf6N2E2_416at 484.240 kb on - strand, within Pf6N2E2_416at 484.240 kb on - strand, within Pf6N2E2_416at 484.251 kb on - strand, within Pf6N2E2_416at 484.251 kb on - strand, within Pf6N2E2_416at 484.324 kb on - strandat 484.324 kb on - strandat 484.324 kb on - strandat 484.394 kb on + strandat 484.394 kb on + strandat 484.395 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml
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482,422 + -0.1
482,422 + -0.4
482,422 + -0.1
482,423 - +0.0
482,423 - -0.5
482,423 - +0.1
482,423 - -1.5
482,423 - -0.1
482,423 - -0.4
482,423 - +0.1
482,423 - -1.7
482,442 + +0.4
482,461 - -1.0
482,461 - +0.3
482,462 + +0.4
482,462 + +0.6
482,462 + -1.7
482,462 + -1.0
482,462 + +0.7
482,462 + +0.1
482,462 + -0.7
482,463 - -0.3
482,463 - -1.3
482,463 - -0.4
482,463 - +0.3
482,463 - -0.2
482,463 - +0.4
482,463 - +0.2
482,463 - -0.3
482,481 + -0.5
482,482 - +0.8
482,482 - +0.1
482,482 - +0.1
482,482 - -0.3
482,482 - +0.6
482,740 + Pf6N2E2_414 0.62 -1.2
482,741 - Pf6N2E2_414 0.62 -0.5
482,741 - Pf6N2E2_414 0.62 -0.8
482,741 - Pf6N2E2_414 0.62 +0.3
482,743 - Pf6N2E2_414 0.62 +1.6
482,743 - Pf6N2E2_414 0.62 +0.2
482,909 - +0.6
482,909 - -1.3
482,909 - +1.0
483,025 - Pf6N2E2_415 0.10 +0.5
483,090 + Pf6N2E2_415 0.18 -0.7
483,090 + Pf6N2E2_415 0.18 +0.1
483,090 + Pf6N2E2_415 0.18 +0.3
483,090 + Pf6N2E2_415 0.18 +0.2
483,091 - Pf6N2E2_415 0.18 +0.6
483,091 - Pf6N2E2_415 0.18 +1.5
483,091 - Pf6N2E2_415 0.18 +0.9
483,240 + Pf6N2E2_415 0.36 +0.1
483,241 - Pf6N2E2_415 0.36 -1.1
483,315 - Pf6N2E2_415 0.45 +0.6
483,315 - Pf6N2E2_415 0.45 +0.2
483,393 + Pf6N2E2_415 0.54 -1.2
483,394 - Pf6N2E2_415 0.54 -0.4
483,465 - Pf6N2E2_415 0.63 +0.6
483,465 - Pf6N2E2_415 0.63 +1.0
483,548 + Pf6N2E2_415 0.73 +0.5
483,548 + Pf6N2E2_415 0.73 +0.4
483,548 + Pf6N2E2_415 0.73 +0.3
483,548 + Pf6N2E2_415 0.73 +0.5
483,548 + Pf6N2E2_415 0.73 +0.8
483,548 + Pf6N2E2_415 0.73 +1.0
483,549 - Pf6N2E2_415 0.73 +0.1
483,549 - Pf6N2E2_415 0.73 +1.3
483,549 - Pf6N2E2_415 0.73 +0.1
483,588 + Pf6N2E2_415 0.78 +0.5
483,597 + Pf6N2E2_415 0.79 -0.0
483,597 + Pf6N2E2_415 0.79 -0.9
483,598 - Pf6N2E2_415 0.79 +0.9
483,612 + Pf6N2E2_415 0.80 +1.4
483,613 - Pf6N2E2_415 0.81 +1.9
483,691 - Pf6N2E2_415 0.90 +0.7
483,691 - Pf6N2E2_415 0.90 -0.5
483,859 + Pf6N2E2_416 0.16 -0.2
483,859 + Pf6N2E2_416 0.16 -0.5
484,239 + Pf6N2E2_416 0.87 +0.5
484,239 + Pf6N2E2_416 0.87 -0.1
484,240 - Pf6N2E2_416 0.87 -0.4
484,240 - Pf6N2E2_416 0.87 +0.9
484,240 - Pf6N2E2_416 0.87 -0.8
484,251 - Pf6N2E2_416 0.89 -1.3
484,251 - Pf6N2E2_416 0.89 +0.2
484,324 - -1.0
484,324 - -0.2
484,324 - +0.2
484,394 + +1.3
484,394 + +0.8
484,395 - +0.3

Or see this region's nucleotide sequence