Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3925 and Pf6N2E2_3926 are separated by 155 nucleotides Pf6N2E2_3926 and Pf6N2E2_3927 are separated by 1 nucleotides
Pf6N2E2_3925 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 4,562,010 to 4,563,368
_3925
Pf6N2E2_3926 - Methionine ABC transporter substrate-binding protein, at 4,563,524 to 4,564,351
_3926
Pf6N2E2_3927 - Methionine ABC transporter ATP-binding protein, at 4,564,353 to 4,565,477
_3927
Position (kb)
4563
4564
4565 Strain fitness (log2 ratio)
-2
-1
0
1 at 4562.524 kb on - strand, within Pf6N2E2_3925 at 4562.524 kb on - strand, within Pf6N2E2_3925 at 4562.524 kb on - strand, within Pf6N2E2_3925 at 4562.586 kb on + strand, within Pf6N2E2_3925 at 4562.586 kb on + strand, within Pf6N2E2_3925 at 4562.587 kb on - strand, within Pf6N2E2_3925 at 4562.587 kb on - strand, within Pf6N2E2_3925 at 4562.587 kb on - strand, within Pf6N2E2_3925 at 4562.587 kb on - strand, within Pf6N2E2_3925 at 4562.587 kb on - strand, within Pf6N2E2_3925 at 4562.587 kb on - strand, within Pf6N2E2_3925 at 4562.634 kb on + strand, within Pf6N2E2_3925 at 4562.634 kb on + strand, within Pf6N2E2_3925 at 4562.635 kb on - strand, within Pf6N2E2_3925 at 4562.635 kb on - strand, within Pf6N2E2_3925 at 4562.635 kb on - strand, within Pf6N2E2_3925 at 4562.922 kb on + strand, within Pf6N2E2_3925 at 4562.922 kb on + strand, within Pf6N2E2_3925 at 4562.923 kb on - strand, within Pf6N2E2_3925 at 4562.923 kb on - strand, within Pf6N2E2_3925 at 4562.923 kb on - strand, within Pf6N2E2_3925 at 4562.923 kb on - strand, within Pf6N2E2_3925 at 4562.923 kb on - strand, within Pf6N2E2_3925 at 4563.010 kb on - strand, within Pf6N2E2_3925 at 4563.010 kb on - strand, within Pf6N2E2_3925 at 4563.108 kb on + strand, within Pf6N2E2_3925 at 4563.108 kb on + strand, within Pf6N2E2_3925 at 4563.108 kb on + strand, within Pf6N2E2_3925 at 4563.108 kb on + strand, within Pf6N2E2_3925 at 4563.109 kb on - strand, within Pf6N2E2_3925 at 4563.109 kb on - strand, within Pf6N2E2_3925 at 4563.109 kb on - strand, within Pf6N2E2_3925 at 4563.219 kb on + strand, within Pf6N2E2_3925 at 4563.220 kb on - strand, within Pf6N2E2_3925 at 4563.220 kb on - strand, within Pf6N2E2_3925 at 4563.270 kb on + strand at 4563.271 kb on - strand at 4563.283 kb on - strand at 4563.331 kb on + strand at 4563.332 kb on - strand at 4563.332 kb on - strand at 4563.332 kb on - strand at 4563.357 kb on + strand at 4563.357 kb on + strand at 4563.518 kb on + strand at 4563.518 kb on + strand at 4563.519 kb on - strand at 4563.535 kb on - strand at 4563.535 kb on - strand at 4563.782 kb on + strand, within Pf6N2E2_3926 at 4563.782 kb on + strand, within Pf6N2E2_3926 at 4563.782 kb on + strand, within Pf6N2E2_3926 at 4564.017 kb on - strand, within Pf6N2E2_3926 at 4564.037 kb on - strand, within Pf6N2E2_3926 at 4564.100 kb on + strand, within Pf6N2E2_3926 at 4564.100 kb on + strand, within Pf6N2E2_3926 at 4564.119 kb on + strand, within Pf6N2E2_3926 at 4564.120 kb on - strand, within Pf6N2E2_3926 at 4564.136 kb on + strand, within Pf6N2E2_3926 at 4564.137 kb on - strand, within Pf6N2E2_3926 at 4564.137 kb on - strand, within Pf6N2E2_3926 at 4564.205 kb on + strand, within Pf6N2E2_3926 at 4564.205 kb on + strand, within Pf6N2E2_3926 at 4564.205 kb on + strand, within Pf6N2E2_3926 at 4564.205 kb on + strand, within Pf6N2E2_3926 at 4564.205 kb on + strand, within Pf6N2E2_3926 at 4564.206 kb on - strand, within Pf6N2E2_3926 at 4564.206 kb on - strand, within Pf6N2E2_3926 at 4564.206 kb on - strand, within Pf6N2E2_3926 at 4564.206 kb on - strand, within Pf6N2E2_3926 at 4564.274 kb on + strand at 4564.274 kb on + strand at 4564.274 kb on + strand at 4564.274 kb on + strand at 4564.275 kb on - strand at 4564.275 kb on - strand at 4564.275 kb on - strand at 4564.328 kb on + strand at 4564.328 kb on + strand at 4564.328 kb on + strand at 4564.329 kb on - strand at 4564.473 kb on + strand, within Pf6N2E2_3927 at 4564.473 kb on + strand, within Pf6N2E2_3927 at 4564.473 kb on + strand, within Pf6N2E2_3927 at 4564.473 kb on + strand, within Pf6N2E2_3927 at 4564.473 kb on + strand, within Pf6N2E2_3927 at 4564.474 kb on - strand, within Pf6N2E2_3927 at 4564.474 kb on - strand, within Pf6N2E2_3927 at 4564.474 kb on - strand, within Pf6N2E2_3927 at 4564.642 kb on + strand, within Pf6N2E2_3927 at 4564.642 kb on + strand, within Pf6N2E2_3927 at 4564.643 kb on - strand, within Pf6N2E2_3927 at 4565.186 kb on - strand, within Pf6N2E2_3927 at 4565.330 kb on - strand, within Pf6N2E2_3927
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 4,562,524 - Pf6N2E2_3925 0.38 +1.0 4,562,524 - Pf6N2E2_3925 0.38 +1.6 4,562,524 - Pf6N2E2_3925 0.38 +0.1 4,562,586 + Pf6N2E2_3925 0.42 +0.1 4,562,586 + Pf6N2E2_3925 0.42 -0.2 4,562,587 - Pf6N2E2_3925 0.42 -1.2 4,562,587 - Pf6N2E2_3925 0.42 -0.3 4,562,587 - Pf6N2E2_3925 0.42 +0.1 4,562,587 - Pf6N2E2_3925 0.42 -0.2 4,562,587 - Pf6N2E2_3925 0.42 -0.1 4,562,587 - Pf6N2E2_3925 0.42 -0.8 4,562,634 + Pf6N2E2_3925 0.46 -0.4 4,562,634 + Pf6N2E2_3925 0.46 -0.3 4,562,635 - Pf6N2E2_3925 0.46 +1.1 4,562,635 - Pf6N2E2_3925 0.46 +0.7 4,562,635 - Pf6N2E2_3925 0.46 -0.1 4,562,922 + Pf6N2E2_3925 0.67 -1.1 4,562,922 + Pf6N2E2_3925 0.67 +0.5 4,562,923 - Pf6N2E2_3925 0.67 +0.6 4,562,923 - Pf6N2E2_3925 0.67 +0.9 4,562,923 - Pf6N2E2_3925 0.67 -0.2 4,562,923 - Pf6N2E2_3925 0.67 +1.2 4,562,923 - Pf6N2E2_3925 0.67 -1.0 4,563,010 - Pf6N2E2_3925 0.74 +0.3 4,563,010 - Pf6N2E2_3925 0.74 +0.9 4,563,108 + Pf6N2E2_3925 0.81 -0.7 4,563,108 + Pf6N2E2_3925 0.81 -0.3 4,563,108 + Pf6N2E2_3925 0.81 -0.4 4,563,108 + Pf6N2E2_3925 0.81 +0.8 4,563,109 - Pf6N2E2_3925 0.81 -1.0 4,563,109 - Pf6N2E2_3925 0.81 +0.1 4,563,109 - Pf6N2E2_3925 0.81 +1.4 4,563,219 + Pf6N2E2_3925 0.89 +0.1 4,563,220 - Pf6N2E2_3925 0.89 +0.6 4,563,220 - Pf6N2E2_3925 0.89 +1.0 4,563,270 + -1.0 4,563,271 - -0.3 4,563,283 - +0.1 4,563,331 + -0.4 4,563,332 - +0.3 4,563,332 - -0.0 4,563,332 - +0.4 4,563,357 + -1.0 4,563,357 + +0.2 4,563,518 + -2.0 4,563,518 + -0.3 4,563,519 - -0.0 4,563,535 - +0.7 4,563,535 - -0.9 4,563,782 + Pf6N2E2_3926 0.31 +0.7 4,563,782 + Pf6N2E2_3926 0.31 +0.2 4,563,782 + Pf6N2E2_3926 0.31 -0.0 4,564,017 - Pf6N2E2_3926 0.60 -1.6 4,564,037 - Pf6N2E2_3926 0.62 -0.8 4,564,100 + Pf6N2E2_3926 0.70 +0.8 4,564,100 + Pf6N2E2_3926 0.70 +0.5 4,564,119 + Pf6N2E2_3926 0.72 +0.7 4,564,120 - Pf6N2E2_3926 0.72 +0.9 4,564,136 + Pf6N2E2_3926 0.74 -0.0 4,564,137 - Pf6N2E2_3926 0.74 +0.2 4,564,137 - Pf6N2E2_3926 0.74 +0.5 4,564,205 + Pf6N2E2_3926 0.82 -1.7 4,564,205 + Pf6N2E2_3926 0.82 -0.9 4,564,205 + Pf6N2E2_3926 0.82 +0.4 4,564,205 + Pf6N2E2_3926 0.82 -0.0 4,564,205 + Pf6N2E2_3926 0.82 -0.1 4,564,206 - Pf6N2E2_3926 0.82 +0.4 4,564,206 - Pf6N2E2_3926 0.82 +0.6 4,564,206 - Pf6N2E2_3926 0.82 +0.4 4,564,206 - Pf6N2E2_3926 0.82 +0.4 4,564,274 + -0.2 4,564,274 + -0.4 4,564,274 + -1.2 4,564,274 + +0.2 4,564,275 - -0.8 4,564,275 - +0.1 4,564,275 - +0.8 4,564,328 + +0.3 4,564,328 + +0.3 4,564,328 + +0.0 4,564,329 - +0.1 4,564,473 + Pf6N2E2_3927 0.11 -0.2 4,564,473 + Pf6N2E2_3927 0.11 -0.4 4,564,473 + Pf6N2E2_3927 0.11 -2.1 4,564,473 + Pf6N2E2_3927 0.11 +0.7 4,564,473 + Pf6N2E2_3927 0.11 -0.4 4,564,474 - Pf6N2E2_3927 0.11 +0.0 4,564,474 - Pf6N2E2_3927 0.11 +0.2 4,564,474 - Pf6N2E2_3927 0.11 +0.2 4,564,642 + Pf6N2E2_3927 0.26 -0.6 4,564,642 + Pf6N2E2_3927 0.26 -0.6 4,564,643 - Pf6N2E2_3927 0.26 +0.6 4,565,186 - Pf6N2E2_3927 0.74 -0.3 4,565,330 - Pf6N2E2_3927 0.87 +0.6
Or see this region's nucleotide sequence