Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2297 and Pf6N2E2_2298 are separated by 386 nucleotides Pf6N2E2_2298 and Pf6N2E2_2299 overlap by 4 nucleotides Pf6N2E2_2299 and Pf6N2E2_2300 are separated by 187 nucleotides
Pf6N2E2_2297 - Erythronate-4-phosphate dehydrogenase (EC 1.1.1.290), at 2,719,560 to 2,720,702
_2297
Pf6N2E2_2298 - Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps, at 2,721,089 to 2,722,423
_2298
Pf6N2E2_2299 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusA, at 2,722,420 to 2,722,671
_2299
Pf6N2E2_2300 - LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM, at 2,722,859 to 2,723,932
_2300
Position (kb)
2721
2722
2723 Strain fitness (log2 ratio)
-1
0
1 at 2720.733 kb on + strand at 2720.734 kb on - strand at 2720.745 kb on + strand at 2720.912 kb on + strand at 2720.912 kb on + strand at 2720.912 kb on + strand at 2720.912 kb on + strand at 2720.913 kb on - strand at 2720.913 kb on - strand at 2720.931 kb on - strand at 2720.931 kb on - strand at 2720.976 kb on + strand at 2720.977 kb on - strand at 2720.977 kb on - strand at 2720.998 kb on - strand at 2720.998 kb on - strand at 2721.031 kb on - strand at 2721.443 kb on + strand, within Pf6N2E2_2298 at 2721.443 kb on + strand, within Pf6N2E2_2298 at 2721.444 kb on - strand, within Pf6N2E2_2298 at 2721.444 kb on - strand, within Pf6N2E2_2298 at 2721.485 kb on + strand, within Pf6N2E2_2298 at 2721.485 kb on + strand, within Pf6N2E2_2298 at 2721.486 kb on - strand, within Pf6N2E2_2298 at 2721.492 kb on + strand, within Pf6N2E2_2298 at 2721.492 kb on + strand, within Pf6N2E2_2298 at 2721.493 kb on - strand, within Pf6N2E2_2298 at 2721.493 kb on - strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.581 kb on + strand, within Pf6N2E2_2298 at 2721.582 kb on - strand, within Pf6N2E2_2298 at 2721.582 kb on - strand, within Pf6N2E2_2298 at 2721.582 kb on - strand, within Pf6N2E2_2298 at 2721.582 kb on - strand, within Pf6N2E2_2298 at 2721.582 kb on - strand, within Pf6N2E2_2298 at 2721.593 kb on + strand, within Pf6N2E2_2298 at 2721.593 kb on + strand, within Pf6N2E2_2298 at 2721.593 kb on + strand, within Pf6N2E2_2298 at 2721.593 kb on + strand, within Pf6N2E2_2298 at 2721.594 kb on - strand, within Pf6N2E2_2298 at 2721.708 kb on - strand, within Pf6N2E2_2298 at 2721.708 kb on - strand, within Pf6N2E2_2298 at 2721.708 kb on - strand, within Pf6N2E2_2298 at 2721.920 kb on + strand, within Pf6N2E2_2298 at 2722.029 kb on - strand, within Pf6N2E2_2298 at 2722.085 kb on + strand, within Pf6N2E2_2298 at 2722.085 kb on + strand, within Pf6N2E2_2298 at 2722.085 kb on + strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.134 kb on - strand, within Pf6N2E2_2298 at 2722.677 kb on - strand at 2722.677 kb on - strand at 2722.926 kb on + strand at 2722.927 kb on - strand at 2722.927 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 2,720,733 + +1.6 2,720,734 - +0.7 2,720,745 + +0.3 2,720,912 + -0.8 2,720,912 + -0.0 2,720,912 + -0.3 2,720,912 + +0.5 2,720,913 - -0.2 2,720,913 - -0.2 2,720,931 - +1.5 2,720,931 - -0.0 2,720,976 + -0.8 2,720,977 - +0.2 2,720,977 - +1.5 2,720,998 - -0.0 2,720,998 - +0.8 2,721,031 - +0.8 2,721,443 + Pf6N2E2_2298 0.27 -1.5 2,721,443 + Pf6N2E2_2298 0.27 +0.2 2,721,444 - Pf6N2E2_2298 0.27 +1.0 2,721,444 - Pf6N2E2_2298 0.27 +0.5 2,721,485 + Pf6N2E2_2298 0.30 -1.2 2,721,485 + Pf6N2E2_2298 0.30 -0.2 2,721,486 - Pf6N2E2_2298 0.30 +1.0 2,721,492 + Pf6N2E2_2298 0.30 +0.0 2,721,492 + Pf6N2E2_2298 0.30 -1.5 2,721,493 - Pf6N2E2_2298 0.30 +0.5 2,721,493 - Pf6N2E2_2298 0.30 +0.3 2,721,581 + Pf6N2E2_2298 0.37 -0.0 2,721,581 + Pf6N2E2_2298 0.37 +0.1 2,721,581 + Pf6N2E2_2298 0.37 +1.6 2,721,581 + Pf6N2E2_2298 0.37 -0.0 2,721,581 + Pf6N2E2_2298 0.37 -0.4 2,721,581 + Pf6N2E2_2298 0.37 +0.5 2,721,581 + Pf6N2E2_2298 0.37 +0.3 2,721,582 - Pf6N2E2_2298 0.37 +0.3 2,721,582 - Pf6N2E2_2298 0.37 +0.4 2,721,582 - Pf6N2E2_2298 0.37 +1.3 2,721,582 - Pf6N2E2_2298 0.37 -0.8 2,721,582 - Pf6N2E2_2298 0.37 +0.6 2,721,593 + Pf6N2E2_2298 0.38 -0.5 2,721,593 + Pf6N2E2_2298 0.38 +0.7 2,721,593 + Pf6N2E2_2298 0.38 +0.6 2,721,593 + Pf6N2E2_2298 0.38 -0.0 2,721,594 - Pf6N2E2_2298 0.38 -0.2 2,721,708 - Pf6N2E2_2298 0.46 +0.5 2,721,708 - Pf6N2E2_2298 0.46 +1.1 2,721,708 - Pf6N2E2_2298 0.46 -0.1 2,721,920 + Pf6N2E2_2298 0.62 -0.4 2,722,029 - Pf6N2E2_2298 0.70 +0.1 2,722,085 + Pf6N2E2_2298 0.75 +0.6 2,722,085 + Pf6N2E2_2298 0.75 -0.6 2,722,085 + Pf6N2E2_2298 0.75 +0.1 2,722,086 - Pf6N2E2_2298 0.75 -0.4 2,722,086 - Pf6N2E2_2298 0.75 -0.4 2,722,086 - Pf6N2E2_2298 0.75 -0.9 2,722,086 - Pf6N2E2_2298 0.75 +0.5 2,722,086 - Pf6N2E2_2298 0.75 -0.0 2,722,134 - Pf6N2E2_2298 0.78 -0.2 2,722,677 - -0.1 2,722,677 - -0.0 2,722,926 + -0.7 2,722,927 - +0.5 2,722,927 - -0.5
Or see this region's nucleotide sequence