Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2038

Experiment: Tetracycline 0.0004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2037 and Pf6N2E2_2038 are separated by 105 nucleotidesPf6N2E2_2038 and Pf6N2E2_2039 overlap by 1 nucleotides Pf6N2E2_2037 - Thioredoxin reductase (EC 1.8.1.9), at 2,425,811 to 2,426,704 _2037 Pf6N2E2_2038 - Transcriptional regulator, TetR family, at 2,426,810 to 2,427,469 _2038 Pf6N2E2_2039 - Sigma-54 dependent transcriptional regulator, at 2,427,469 to 2,428,884 _2039 Position (kb) 2426 2427 2428Strain fitness (log2 ratio) -2 -1 0 1at 2425.915 kb on - strand, within Pf6N2E2_2037at 2425.915 kb on - strand, within Pf6N2E2_2037at 2426.229 kb on + strand, within Pf6N2E2_2037at 2426.241 kb on + strand, within Pf6N2E2_2037at 2426.241 kb on + strand, within Pf6N2E2_2037at 2426.241 kb on + strand, within Pf6N2E2_2037at 2426.241 kb on + strand, within Pf6N2E2_2037at 2426.241 kb on + strand, within Pf6N2E2_2037at 2426.242 kb on - strand, within Pf6N2E2_2037at 2426.242 kb on - strand, within Pf6N2E2_2037at 2426.242 kb on - strand, within Pf6N2E2_2037at 2426.348 kb on - strand, within Pf6N2E2_2037at 2426.386 kb on - strand, within Pf6N2E2_2037at 2426.386 kb on - strand, within Pf6N2E2_2037at 2426.386 kb on - strand, within Pf6N2E2_2037at 2426.386 kb on - strand, within Pf6N2E2_2037at 2426.386 kb on - strand, within Pf6N2E2_2037at 2426.677 kb on + strandat 2426.869 kb on + strandat 2426.870 kb on - strandat 2426.870 kb on - strandat 2426.870 kb on - strandat 2426.870 kb on - strandat 2426.870 kb on - strandat 2426.872 kb on - strandat 2426.872 kb on - strandat 2427.197 kb on + strand, within Pf6N2E2_2038at 2427.197 kb on + strand, within Pf6N2E2_2038at 2427.215 kb on + strand, within Pf6N2E2_2038at 2427.215 kb on + strand, within Pf6N2E2_2038at 2427.216 kb on - strand, within Pf6N2E2_2038at 2427.393 kb on - strand, within Pf6N2E2_2038at 2427.393 kb on - strand, within Pf6N2E2_2038at 2427.393 kb on - strand, within Pf6N2E2_2038at 2427.393 kb on - strand, within Pf6N2E2_2038at 2427.496 kb on + strandat 2427.496 kb on + strandat 2427.610 kb on + strandat 2427.610 kb on + strandat 2427.862 kb on + strand, within Pf6N2E2_2039at 2427.925 kb on + strand, within Pf6N2E2_2039at 2427.926 kb on - strand, within Pf6N2E2_2039at 2428.228 kb on - strand, within Pf6N2E2_2039at 2428.306 kb on + strand, within Pf6N2E2_2039at 2428.417 kb on + strand, within Pf6N2E2_2039

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Per-strain Table

Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml
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2,425,915 - Pf6N2E2_2037 0.12 +1.3
2,425,915 - Pf6N2E2_2037 0.12 -2.5
2,426,229 + Pf6N2E2_2037 0.47 -0.2
2,426,241 + Pf6N2E2_2037 0.48 -0.0
2,426,241 + Pf6N2E2_2037 0.48 +1.1
2,426,241 + Pf6N2E2_2037 0.48 +0.0
2,426,241 + Pf6N2E2_2037 0.48 -1.6
2,426,241 + Pf6N2E2_2037 0.48 +0.8
2,426,242 - Pf6N2E2_2037 0.48 +0.3
2,426,242 - Pf6N2E2_2037 0.48 -0.3
2,426,242 - Pf6N2E2_2037 0.48 -0.2
2,426,348 - Pf6N2E2_2037 0.60 +0.1
2,426,386 - Pf6N2E2_2037 0.64 +0.9
2,426,386 - Pf6N2E2_2037 0.64 -0.4
2,426,386 - Pf6N2E2_2037 0.64 -1.1
2,426,386 - Pf6N2E2_2037 0.64 +0.9
2,426,386 - Pf6N2E2_2037 0.64 +0.4
2,426,677 + +0.3
2,426,869 + -2.3
2,426,870 - -0.9
2,426,870 - -0.1
2,426,870 - -0.3
2,426,870 - -0.4
2,426,870 - -0.1
2,426,872 - -1.0
2,426,872 - +0.0
2,427,197 + Pf6N2E2_2038 0.59 -1.1
2,427,197 + Pf6N2E2_2038 0.59 -1.9
2,427,215 + Pf6N2E2_2038 0.61 -0.6
2,427,215 + Pf6N2E2_2038 0.61 -0.4
2,427,216 - Pf6N2E2_2038 0.62 -0.0
2,427,393 - Pf6N2E2_2038 0.88 -2.4
2,427,393 - Pf6N2E2_2038 0.88 +0.1
2,427,393 - Pf6N2E2_2038 0.88 -1.4
2,427,393 - Pf6N2E2_2038 0.88 -1.0
2,427,496 + -0.9
2,427,496 + +0.0
2,427,610 + +0.0
2,427,610 + -0.2
2,427,862 + Pf6N2E2_2039 0.28 +0.1
2,427,925 + Pf6N2E2_2039 0.32 -0.1
2,427,926 - Pf6N2E2_2039 0.32 -2.0
2,428,228 - Pf6N2E2_2039 0.54 +0.7
2,428,306 + Pf6N2E2_2039 0.59 +0.9
2,428,417 + Pf6N2E2_2039 0.67 -0.0

Or see this region's nucleotide sequence