Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2037 and Pf6N2E2_2038 are separated by 105 nucleotides Pf6N2E2_2038 and Pf6N2E2_2039 overlap by 1 nucleotides
Pf6N2E2_2037 - Thioredoxin reductase (EC 1.8.1.9), at 2,425,811 to 2,426,704
_2037
Pf6N2E2_2038 - Transcriptional regulator, TetR family, at 2,426,810 to 2,427,469
_2038
Pf6N2E2_2039 - Sigma-54 dependent transcriptional regulator, at 2,427,469 to 2,428,884
_2039
Position (kb)
2426
2427
2428 Strain fitness (log2 ratio)
-2
-1
0
1 at 2425.915 kb on - strand, within Pf6N2E2_2037 at 2425.915 kb on - strand, within Pf6N2E2_2037 at 2426.229 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.241 kb on + strand, within Pf6N2E2_2037 at 2426.242 kb on - strand, within Pf6N2E2_2037 at 2426.242 kb on - strand, within Pf6N2E2_2037 at 2426.242 kb on - strand, within Pf6N2E2_2037 at 2426.348 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.386 kb on - strand, within Pf6N2E2_2037 at 2426.677 kb on + strand at 2426.869 kb on + strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.870 kb on - strand at 2426.872 kb on - strand at 2426.872 kb on - strand at 2427.197 kb on + strand, within Pf6N2E2_2038 at 2427.197 kb on + strand, within Pf6N2E2_2038 at 2427.215 kb on + strand, within Pf6N2E2_2038 at 2427.215 kb on + strand, within Pf6N2E2_2038 at 2427.216 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.393 kb on - strand, within Pf6N2E2_2038 at 2427.496 kb on + strand at 2427.496 kb on + strand at 2427.610 kb on + strand at 2427.610 kb on + strand at 2427.862 kb on + strand, within Pf6N2E2_2039 at 2427.925 kb on + strand, within Pf6N2E2_2039 at 2427.926 kb on - strand, within Pf6N2E2_2039 at 2428.228 kb on - strand, within Pf6N2E2_2039 at 2428.306 kb on + strand, within Pf6N2E2_2039 at 2428.417 kb on + strand, within Pf6N2E2_2039
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 2,425,915 - Pf6N2E2_2037 0.12 +1.3 2,425,915 - Pf6N2E2_2037 0.12 -2.5 2,426,229 + Pf6N2E2_2037 0.47 -0.2 2,426,241 + Pf6N2E2_2037 0.48 -0.0 2,426,241 + Pf6N2E2_2037 0.48 +1.1 2,426,241 + Pf6N2E2_2037 0.48 +0.0 2,426,241 + Pf6N2E2_2037 0.48 -1.6 2,426,241 + Pf6N2E2_2037 0.48 +0.8 2,426,242 - Pf6N2E2_2037 0.48 +0.3 2,426,242 - Pf6N2E2_2037 0.48 -0.3 2,426,242 - Pf6N2E2_2037 0.48 -0.2 2,426,348 - Pf6N2E2_2037 0.60 +0.1 2,426,386 - Pf6N2E2_2037 0.64 +0.9 2,426,386 - Pf6N2E2_2037 0.64 -0.4 2,426,386 - Pf6N2E2_2037 0.64 -1.1 2,426,386 - Pf6N2E2_2037 0.64 +0.9 2,426,386 - Pf6N2E2_2037 0.64 +0.4 2,426,677 + +0.3 2,426,869 + -2.3 2,426,870 - -0.9 2,426,870 - -0.1 2,426,870 - -0.3 2,426,870 - -0.4 2,426,870 - -0.1 2,426,872 - -1.0 2,426,872 - +0.0 2,427,197 + Pf6N2E2_2038 0.59 -1.1 2,427,197 + Pf6N2E2_2038 0.59 -1.9 2,427,215 + Pf6N2E2_2038 0.61 -0.6 2,427,215 + Pf6N2E2_2038 0.61 -0.4 2,427,216 - Pf6N2E2_2038 0.62 -0.0 2,427,393 - Pf6N2E2_2038 0.88 -2.4 2,427,393 - Pf6N2E2_2038 0.88 +0.1 2,427,393 - Pf6N2E2_2038 0.88 -1.4 2,427,393 - Pf6N2E2_2038 0.88 -1.0 2,427,496 + -0.9 2,427,496 + +0.0 2,427,610 + +0.0 2,427,610 + -0.2 2,427,862 + Pf6N2E2_2039 0.28 +0.1 2,427,925 + Pf6N2E2_2039 0.32 -0.1 2,427,926 - Pf6N2E2_2039 0.32 -2.0 2,428,228 - Pf6N2E2_2039 0.54 +0.7 2,428,306 + Pf6N2E2_2039 0.59 +0.9 2,428,417 + Pf6N2E2_2039 0.67 -0.0
Or see this region's nucleotide sequence