Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1805 and Pf6N2E2_1806 are separated by 701 nucleotides Pf6N2E2_1806 and Pf6N2E2_1807 are separated by 58 nucleotides
Pf6N2E2_1805 - Multidrug resistance protein B, at 2,134,884 to 2,136,029
_1805
Pf6N2E2_1806 - hypothetical protein, at 2,136,731 to 2,137,237
_1806
Pf6N2E2_1807 - hypothetical protein, at 2,137,296 to 2,139,569
_1807
Position (kb)
2136
2137
2138 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2135.856 kb on + strand, within Pf6N2E2_1805 at 2135.856 kb on + strand, within Pf6N2E2_1805 at 2135.856 kb on + strand, within Pf6N2E2_1805 at 2135.928 kb on - strand at 2135.949 kb on + strand at 2135.950 kb on - strand at 2136.086 kb on + strand at 2136.087 kb on - strand at 2136.087 kb on - strand at 2136.122 kb on - strand at 2136.141 kb on + strand at 2136.141 kb on + strand at 2136.204 kb on - strand at 2136.204 kb on - strand at 2136.207 kb on + strand at 2136.207 kb on + strand at 2136.208 kb on - strand at 2136.208 kb on - strand at 2136.384 kb on + strand at 2136.385 kb on - strand at 2136.758 kb on + strand at 2136.921 kb on - strand, within Pf6N2E2_1806 at 2137.073 kb on + strand, within Pf6N2E2_1806 at 2137.188 kb on + strand at 2137.188 kb on + strand at 2137.260 kb on + strand at 2137.260 kb on + strand at 2137.260 kb on + strand at 2137.260 kb on + strand at 2137.261 kb on - strand at 2137.261 kb on - strand at 2137.261 kb on - strand at 2137.261 kb on - strand at 2137.262 kb on + strand at 2137.262 kb on + strand at 2137.263 kb on - strand at 2137.263 kb on - strand at 2137.263 kb on - strand at 2137.267 kb on - strand at 2137.267 kb on - strand at 2137.267 kb on - strand at 2137.554 kb on + strand, within Pf6N2E2_1807 at 2137.956 kb on - strand, within Pf6N2E2_1807 at 2138.172 kb on + strand, within Pf6N2E2_1807 at 2138.173 kb on - strand, within Pf6N2E2_1807 at 2138.173 kb on - strand, within Pf6N2E2_1807 at 2138.179 kb on + strand, within Pf6N2E2_1807
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 2,135,856 + Pf6N2E2_1805 0.85 -0.5 2,135,856 + Pf6N2E2_1805 0.85 -0.3 2,135,856 + Pf6N2E2_1805 0.85 +1.1 2,135,928 - -0.4 2,135,949 + +0.0 2,135,950 - +1.0 2,136,086 + -0.1 2,136,087 - +0.2 2,136,087 - +0.3 2,136,122 - -2.8 2,136,141 + +1.4 2,136,141 + -1.2 2,136,204 - +1.1 2,136,204 - +0.7 2,136,207 + +0.5 2,136,207 + +0.5 2,136,208 - +0.4 2,136,208 - -0.7 2,136,384 + +0.5 2,136,385 - -0.5 2,136,758 + +0.2 2,136,921 - Pf6N2E2_1806 0.37 -0.1 2,137,073 + Pf6N2E2_1806 0.67 -2.1 2,137,188 + -0.7 2,137,188 + -0.4 2,137,260 + +0.8 2,137,260 + -0.7 2,137,260 + -0.1 2,137,260 + +1.4 2,137,261 - -0.7 2,137,261 - +0.3 2,137,261 - -0.0 2,137,261 - -1.5 2,137,262 + -1.0 2,137,262 + +0.3 2,137,263 - +1.1 2,137,263 - +1.0 2,137,263 - +1.1 2,137,267 - -0.0 2,137,267 - -0.6 2,137,267 - -0.0 2,137,554 + Pf6N2E2_1807 0.11 -0.7 2,137,956 - Pf6N2E2_1807 0.29 +0.8 2,138,172 + Pf6N2E2_1807 0.39 -0.1 2,138,173 - Pf6N2E2_1807 0.39 -0.2 2,138,173 - Pf6N2E2_1807 0.39 -0.4 2,138,179 + Pf6N2E2_1807 0.39 -0.8
Or see this region's nucleotide sequence