Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1660 and Pf6N2E2_1661 are separated by 3 nucleotides Pf6N2E2_1661 and Pf6N2E2_1662 are separated by 132 nucleotides
Pf6N2E2_1660 - RND efflux system, membrane fusion protein CmeA, at 1,964,569 to 1,965,738
_1660
Pf6N2E2_1661 - RND efflux system, inner membrane transporter CmeB, at 1,965,742 to 1,968,837
_1661
Pf6N2E2_1662 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase., at 1,968,970 to 1,969,818
_1662
Position (kb) 1965
1966
1967
1968
1969 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1965.105 kb on + strand, within Pf6N2E2_1660 at 1965.736 kb on + strand at 1965.736 kb on + strand at 1965.737 kb on - strand at 1965.737 kb on - strand at 1965.737 kb on - strand at 1965.877 kb on + strand at 1965.970 kb on + strand at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.562 kb on - strand, within Pf6N2E2_1661 at 1966.684 kb on + strand, within Pf6N2E2_1661 at 1966.685 kb on - strand, within Pf6N2E2_1661 at 1966.705 kb on + strand, within Pf6N2E2_1661 at 1966.762 kb on + strand, within Pf6N2E2_1661 at 1966.762 kb on + strand, within Pf6N2E2_1661 at 1966.762 kb on + strand, within Pf6N2E2_1661 at 1966.762 kb on + strand, within Pf6N2E2_1661 at 1966.762 kb on + strand, within Pf6N2E2_1661 at 1966.762 kb on + strand, within Pf6N2E2_1661 at 1967.137 kb on + strand, within Pf6N2E2_1661 at 1967.138 kb on - strand, within Pf6N2E2_1661 at 1967.368 kb on + strand, within Pf6N2E2_1661 at 1967.369 kb on - strand, within Pf6N2E2_1661 at 1967.446 kb on + strand, within Pf6N2E2_1661 at 1967.446 kb on + strand, within Pf6N2E2_1661 at 1967.494 kb on - strand, within Pf6N2E2_1661 at 1968.038 kb on + strand, within Pf6N2E2_1661 at 1968.178 kb on + strand, within Pf6N2E2_1661 at 1968.178 kb on + strand, within Pf6N2E2_1661 at 1968.178 kb on + strand, within Pf6N2E2_1661 at 1968.398 kb on - strand, within Pf6N2E2_1661 at 1968.461 kb on + strand, within Pf6N2E2_1661 at 1969.222 kb on + strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.409 kb on + strand, within Pf6N2E2_1662 at 1969.410 kb on - strand, within Pf6N2E2_1662 at 1969.410 kb on - strand, within Pf6N2E2_1662 at 1969.521 kb on + strand, within Pf6N2E2_1662 at 1969.522 kb on - strand, within Pf6N2E2_1662 at 1969.810 kb on + strand at 1969.810 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 1,965,105 + Pf6N2E2_1660 0.46 -2.1 1,965,736 + +0.1 1,965,736 + -1.6 1,965,737 - -2.2 1,965,737 - -2.0 1,965,737 - +1.5 1,965,877 + -1.5 1,965,970 + -2.9 1,966,485 - Pf6N2E2_1661 0.24 -2.1 1,966,485 - Pf6N2E2_1661 0.24 -2.7 1,966,485 - Pf6N2E2_1661 0.24 -1.1 1,966,485 - Pf6N2E2_1661 0.24 -2.7 1,966,562 - Pf6N2E2_1661 0.26 -2.6 1,966,684 + Pf6N2E2_1661 0.30 -1.6 1,966,685 - Pf6N2E2_1661 0.30 -2.3 1,966,705 + Pf6N2E2_1661 0.31 -0.7 1,966,762 + Pf6N2E2_1661 0.33 -0.5 1,966,762 + Pf6N2E2_1661 0.33 -1.7 1,966,762 + Pf6N2E2_1661 0.33 -0.8 1,966,762 + Pf6N2E2_1661 0.33 +0.7 1,966,762 + Pf6N2E2_1661 0.33 -1.8 1,966,762 + Pf6N2E2_1661 0.33 -1.5 1,967,137 + Pf6N2E2_1661 0.45 -2.1 1,967,138 - Pf6N2E2_1661 0.45 -0.3 1,967,368 + Pf6N2E2_1661 0.53 -1.9 1,967,369 - Pf6N2E2_1661 0.53 -2.3 1,967,446 + Pf6N2E2_1661 0.55 -2.2 1,967,446 + Pf6N2E2_1661 0.55 -0.7 1,967,494 - Pf6N2E2_1661 0.57 -2.4 1,968,038 + Pf6N2E2_1661 0.74 -1.1 1,968,178 + Pf6N2E2_1661 0.79 -0.9 1,968,178 + Pf6N2E2_1661 0.79 -3.0 1,968,178 + Pf6N2E2_1661 0.79 -3.0 1,968,398 - Pf6N2E2_1661 0.86 +1.1 1,968,461 + Pf6N2E2_1661 0.88 -1.5 1,969,222 + Pf6N2E2_1662 0.30 -0.9 1,969,223 - Pf6N2E2_1662 0.30 -0.6 1,969,223 - Pf6N2E2_1662 0.30 -0.5 1,969,223 - Pf6N2E2_1662 0.30 -0.6 1,969,409 + Pf6N2E2_1662 0.52 +0.7 1,969,410 - Pf6N2E2_1662 0.52 +0.4 1,969,410 - Pf6N2E2_1662 0.52 +0.4 1,969,521 + Pf6N2E2_1662 0.65 -0.6 1,969,522 - Pf6N2E2_1662 0.65 +0.7 1,969,810 + +1.9 1,969,810 + +0.3
Or see this region's nucleotide sequence