Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1657 and Pf6N2E2_1658 are separated by 78 nucleotides Pf6N2E2_1658 and Pf6N2E2_1659 are separated by 18 nucleotides Pf6N2E2_1659 and Pf6N2E2_1660 are separated by 113 nucleotides Pf6N2E2_1660 and Pf6N2E2_1661 are separated by 3 nucleotides
Pf6N2E2_1657 - NAD glycohydrolase, hvnA; Halovibrin, at 1,962,761 to 1,963,996
_1657
Pf6N2E2_1658 - hypothetical protein, at 1,964,075 to 1,964,323
_1658
Pf6N2E2_1659 - Inositol transport system sugar-binding protein, at 1,964,342 to 1,964,455
_1659
Pf6N2E2_1660 - RND efflux system, membrane fusion protein CmeA, at 1,964,569 to 1,965,738
_1660
Pf6N2E2_1661 - RND efflux system, inner membrane transporter CmeB, at 1,965,742 to 1,968,837
_1661
Position (kb) 1964
1965
1966 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1963.664 kb on + strand, within Pf6N2E2_1657 at 1963.665 kb on - strand, within Pf6N2E2_1657 at 1963.678 kb on + strand, within Pf6N2E2_1657 at 1963.679 kb on - strand, within Pf6N2E2_1657 at 1963.679 kb on - strand, within Pf6N2E2_1657 at 1963.799 kb on + strand, within Pf6N2E2_1657 at 1963.799 kb on + strand, within Pf6N2E2_1657 at 1963.799 kb on + strand, within Pf6N2E2_1657 at 1963.835 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.869 kb on + strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.870 kb on - strand, within Pf6N2E2_1657 at 1963.949 kb on + strand at 1963.949 kb on + strand at 1963.950 kb on - strand at 1963.979 kb on + strand at 1963.980 kb on - strand at 1963.980 kb on - strand at 1964.001 kb on + strand at 1964.097 kb on - strand at 1964.302 kb on - strand at 1964.313 kb on - strand at 1964.313 kb on - strand at 1964.366 kb on - strand, within Pf6N2E2_1659 at 1964.481 kb on - strand at 1964.481 kb on - strand at 1964.502 kb on - strand at 1964.723 kb on + strand, within Pf6N2E2_1660 at 1964.723 kb on + strand, within Pf6N2E2_1660 at 1964.723 kb on + strand, within Pf6N2E2_1660 at 1965.105 kb on + strand, within Pf6N2E2_1660 at 1965.736 kb on + strand at 1965.736 kb on + strand at 1965.737 kb on - strand at 1965.737 kb on - strand at 1965.737 kb on - strand at 1965.877 kb on + strand at 1965.970 kb on + strand at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.562 kb on - strand, within Pf6N2E2_1661 at 1966.684 kb on + strand, within Pf6N2E2_1661 at 1966.685 kb on - strand, within Pf6N2E2_1661 at 1966.705 kb on + strand, within Pf6N2E2_1661
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 1,963,664 + Pf6N2E2_1657 0.73 -0.6 1,963,665 - Pf6N2E2_1657 0.73 -0.4 1,963,678 + Pf6N2E2_1657 0.74 -0.6 1,963,679 - Pf6N2E2_1657 0.74 -1.6 1,963,679 - Pf6N2E2_1657 0.74 -1.3 1,963,799 + Pf6N2E2_1657 0.84 -1.3 1,963,799 + Pf6N2E2_1657 0.84 -0.7 1,963,799 + Pf6N2E2_1657 0.84 +1.1 1,963,835 + Pf6N2E2_1657 0.87 -0.0 1,963,869 + Pf6N2E2_1657 0.90 +0.1 1,963,869 + Pf6N2E2_1657 0.90 +2.0 1,963,869 + Pf6N2E2_1657 0.90 +1.0 1,963,869 + Pf6N2E2_1657 0.90 -0.6 1,963,869 + Pf6N2E2_1657 0.90 -1.5 1,963,869 + Pf6N2E2_1657 0.90 -0.7 1,963,869 + Pf6N2E2_1657 0.90 +0.9 1,963,870 - Pf6N2E2_1657 0.90 -1.6 1,963,870 - Pf6N2E2_1657 0.90 +0.7 1,963,870 - Pf6N2E2_1657 0.90 +1.0 1,963,870 - Pf6N2E2_1657 0.90 -1.0 1,963,870 - Pf6N2E2_1657 0.90 +1.6 1,963,870 - Pf6N2E2_1657 0.90 +0.5 1,963,870 - Pf6N2E2_1657 0.90 +1.1 1,963,870 - Pf6N2E2_1657 0.90 -0.4 1,963,870 - Pf6N2E2_1657 0.90 -0.1 1,963,870 - Pf6N2E2_1657 0.90 +0.4 1,963,949 + +0.3 1,963,949 + -0.7 1,963,950 - -1.0 1,963,979 + -0.1 1,963,980 - -0.5 1,963,980 - +0.1 1,964,001 + +0.2 1,964,097 - -0.0 1,964,302 - -0.3 1,964,313 - -0.4 1,964,313 - +0.1 1,964,366 - Pf6N2E2_1659 0.21 -1.4 1,964,481 - -0.6 1,964,481 - -1.7 1,964,502 - -1.1 1,964,723 + Pf6N2E2_1660 0.13 -2.4 1,964,723 + Pf6N2E2_1660 0.13 -0.9 1,964,723 + Pf6N2E2_1660 0.13 -0.4 1,965,105 + Pf6N2E2_1660 0.46 -2.1 1,965,736 + +0.1 1,965,736 + -1.6 1,965,737 - -2.2 1,965,737 - -2.0 1,965,737 - +1.5 1,965,877 + -1.5 1,965,970 + -2.9 1,966,485 - Pf6N2E2_1661 0.24 -2.1 1,966,485 - Pf6N2E2_1661 0.24 -2.7 1,966,485 - Pf6N2E2_1661 0.24 -1.1 1,966,485 - Pf6N2E2_1661 0.24 -2.7 1,966,562 - Pf6N2E2_1661 0.26 -2.6 1,966,684 + Pf6N2E2_1661 0.30 -1.6 1,966,685 - Pf6N2E2_1661 0.30 -2.3 1,966,705 + Pf6N2E2_1661 0.31 -0.7
Or see this region's nucleotide sequence