Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1447 and Pf6N2E2_1448 are separated by 211 nucleotides Pf6N2E2_1448 and Pf6N2E2_1449 overlap by 2 nucleotides Pf6N2E2_1449 and Pf6N2E2_1450 are separated by 39 nucleotides Pf6N2E2_1450 and Pf6N2E2_1451 are separated by 43 nucleotides
Pf6N2E2_1447 - LysR family transcriptional regulatory protein, at 1,697,265 to 1,697,858
_1447
Pf6N2E2_1448 - hypothetical protein, at 1,698,070 to 1,698,411
_1448
Pf6N2E2_1449 - hypothetical protein, at 1,698,410 to 1,698,523
_1449
Pf6N2E2_1450 - FIG00960438: hypothetical protein, at 1,698,563 to 1,698,799
_1450
Pf6N2E2_1451 - hypothetical protein, at 1,698,843 to 1,699,817
_1451
Position (kb)
1698
1699 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1697.745 kb on + strand, within Pf6N2E2_1447 at 1697.745 kb on + strand, within Pf6N2E2_1447 at 1697.746 kb on - strand, within Pf6N2E2_1447 at 1697.746 kb on - strand, within Pf6N2E2_1447 at 1697.746 kb on - strand, within Pf6N2E2_1447 at 1697.746 kb on - strand, within Pf6N2E2_1447 at 1697.756 kb on + strand, within Pf6N2E2_1447 at 1697.795 kb on + strand, within Pf6N2E2_1447 at 1697.854 kb on + strand at 1697.854 kb on + strand at 1697.854 kb on + strand at 1697.854 kb on + strand at 1697.854 kb on + strand at 1697.854 kb on + strand at 1697.855 kb on - strand at 1697.855 kb on - strand at 1697.856 kb on + strand at 1697.857 kb on - strand at 1698.062 kb on - strand at 1698.141 kb on - strand, within Pf6N2E2_1448 at 1698.173 kb on + strand, within Pf6N2E2_1448 at 1698.173 kb on + strand, within Pf6N2E2_1448 at 1698.173 kb on + strand, within Pf6N2E2_1448 at 1698.174 kb on - strand, within Pf6N2E2_1448 at 1698.296 kb on + strand, within Pf6N2E2_1448 at 1698.401 kb on - strand at 1698.435 kb on + strand, within Pf6N2E2_1449 at 1698.515 kb on + strand at 1698.516 kb on - strand at 1698.521 kb on + strand at 1698.522 kb on - strand at 1698.527 kb on + strand at 1698.527 kb on + strand at 1698.528 kb on - strand at 1698.528 kb on - strand at 1698.558 kb on + strand at 1698.558 kb on + strand at 1698.626 kb on + strand, within Pf6N2E2_1450 at 1698.639 kb on - strand, within Pf6N2E2_1450 at 1698.797 kb on + strand at 1698.939 kb on + strand at 1699.049 kb on - strand, within Pf6N2E2_1451 at 1699.268 kb on + strand, within Pf6N2E2_1451 at 1699.268 kb on + strand, within Pf6N2E2_1451 at 1699.268 kb on + strand, within Pf6N2E2_1451 at 1699.268 kb on + strand, within Pf6N2E2_1451 at 1699.269 kb on - strand, within Pf6N2E2_1451 at 1699.336 kb on + strand, within Pf6N2E2_1451 at 1699.336 kb on + strand, within Pf6N2E2_1451 at 1699.337 kb on - strand, within Pf6N2E2_1451 at 1699.453 kb on + strand, within Pf6N2E2_1451 at 1699.453 kb on + strand, within Pf6N2E2_1451 at 1699.454 kb on - strand, within Pf6N2E2_1451 at 1699.457 kb on + strand, within Pf6N2E2_1451 at 1699.750 kb on - strand at 1699.750 kb on - strand at 1699.750 kb on - strand at 1699.750 kb on - strand at 1699.751 kb on + strand at 1699.752 kb on - strand at 1699.752 kb on - strand at 1699.752 kb on - strand at 1699.752 kb on - strand at 1699.753 kb on + strand at 1699.754 kb on - strand at 1699.754 kb on - strand at 1699.754 kb on - strand at 1699.754 kb on - strand at 1699.754 kb on - strand at 1699.763 kb on - strand at 1699.763 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 1,697,745 + Pf6N2E2_1447 0.81 +0.2 1,697,745 + Pf6N2E2_1447 0.81 +0.2 1,697,746 - Pf6N2E2_1447 0.81 +0.0 1,697,746 - Pf6N2E2_1447 0.81 +0.2 1,697,746 - Pf6N2E2_1447 0.81 +0.0 1,697,746 - Pf6N2E2_1447 0.81 -0.1 1,697,756 + Pf6N2E2_1447 0.83 -0.8 1,697,795 + Pf6N2E2_1447 0.89 +0.2 1,697,854 + +0.2 1,697,854 + -0.0 1,697,854 + -0.7 1,697,854 + -0.9 1,697,854 + +0.2 1,697,854 + +0.3 1,697,855 - +0.7 1,697,855 - -1.2 1,697,856 + -2.1 1,697,857 - -2.1 1,698,062 - +1.3 1,698,141 - Pf6N2E2_1448 0.21 +0.8 1,698,173 + Pf6N2E2_1448 0.30 +0.3 1,698,173 + Pf6N2E2_1448 0.30 -0.1 1,698,173 + Pf6N2E2_1448 0.30 +0.2 1,698,174 - Pf6N2E2_1448 0.30 -0.2 1,698,296 + Pf6N2E2_1448 0.66 +0.1 1,698,401 - -0.4 1,698,435 + Pf6N2E2_1449 0.22 +0.7 1,698,515 + -1.4 1,698,516 - +0.4 1,698,521 + +0.1 1,698,522 - +1.5 1,698,527 + -0.9 1,698,527 + +0.3 1,698,528 - +0.5 1,698,528 - +0.4 1,698,558 + +0.1 1,698,558 + +0.7 1,698,626 + Pf6N2E2_1450 0.27 +0.2 1,698,639 - Pf6N2E2_1450 0.32 -1.1 1,698,797 + -0.2 1,698,939 + +0.1 1,699,049 - Pf6N2E2_1451 0.21 -0.9 1,699,268 + Pf6N2E2_1451 0.44 +0.4 1,699,268 + Pf6N2E2_1451 0.44 +1.6 1,699,268 + Pf6N2E2_1451 0.44 -0.3 1,699,268 + Pf6N2E2_1451 0.44 -1.6 1,699,269 - Pf6N2E2_1451 0.44 +1.1 1,699,336 + Pf6N2E2_1451 0.51 -0.2 1,699,336 + Pf6N2E2_1451 0.51 -0.3 1,699,337 - Pf6N2E2_1451 0.51 +0.3 1,699,453 + Pf6N2E2_1451 0.63 +0.7 1,699,453 + Pf6N2E2_1451 0.63 +0.2 1,699,454 - Pf6N2E2_1451 0.63 +0.2 1,699,457 + Pf6N2E2_1451 0.63 +0.4 1,699,750 - -0.4 1,699,750 - +0.3 1,699,750 - +1.3 1,699,750 - -0.1 1,699,751 + +0.1 1,699,752 - -0.3 1,699,752 - -1.0 1,699,752 - +0.3 1,699,752 - +1.2 1,699,753 + +0.9 1,699,754 - -1.0 1,699,754 - +0.9 1,699,754 - +0.2 1,699,754 - +2.3 1,699,754 - +0.5 1,699,763 - -0.9 1,699,763 - +0.7
Or see this region's nucleotide sequence