Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_142 and Pf6N2E2_143 are separated by 177 nucleotides Pf6N2E2_143 and Pf6N2E2_144 are separated by 83 nucleotides Pf6N2E2_144 and Pf6N2E2_145 are separated by 17 nucleotides
Pf6N2E2_142 - Exodeoxyribonuclease III (EC 3.1.11.2), at 193,732 to 194,544
_142
Pf6N2E2_143 - Histone acetyltransferase HPA2 and related acetyltransferases, at 194,722 to 195,372
_143
Pf6N2E2_144 - Uncharacterized protein YtfM precursor, at 195,456 to 197,165
_144
Pf6N2E2_145 - putative exported protein, at 197,183 to 200,836
_145
Position (kb) 195
196
197
198 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 194.574 kb on + strand at 194.574 kb on + strand at 194.575 kb on - strand at 194.575 kb on - strand at 194.575 kb on - strand at 194.641 kb on + strand at 194.641 kb on + strand at 194.641 kb on + strand at 194.642 kb on - strand at 194.670 kb on + strand at 194.671 kb on - strand at 194.677 kb on + strand at 194.757 kb on + strand at 194.757 kb on + strand at 194.757 kb on + strand at 194.758 kb on - strand at 194.773 kb on + strand at 194.801 kb on - strand, within Pf6N2E2_143 at 194.801 kb on - strand, within Pf6N2E2_143 at 194.801 kb on - strand, within Pf6N2E2_143 at 194.801 kb on - strand, within Pf6N2E2_143 at 194.833 kb on + strand, within Pf6N2E2_143 at 194.833 kb on + strand, within Pf6N2E2_143 at 194.834 kb on - strand, within Pf6N2E2_143 at 194.834 kb on - strand, within Pf6N2E2_143 at 194.834 kb on - strand, within Pf6N2E2_143 at 194.834 kb on - strand, within Pf6N2E2_143 at 195.074 kb on - strand, within Pf6N2E2_143 at 195.074 kb on - strand, within Pf6N2E2_143 at 195.119 kb on + strand, within Pf6N2E2_143 at 195.119 kb on + strand, within Pf6N2E2_143 at 195.119 kb on + strand, within Pf6N2E2_143 at 195.119 kb on + strand, within Pf6N2E2_143 at 195.119 kb on + strand, within Pf6N2E2_143 at 195.120 kb on - strand, within Pf6N2E2_143 at 195.120 kb on - strand, within Pf6N2E2_143 at 195.120 kb on - strand, within Pf6N2E2_143 at 195.192 kb on + strand, within Pf6N2E2_143 at 195.192 kb on + strand, within Pf6N2E2_143 at 195.192 kb on + strand, within Pf6N2E2_143 at 195.192 kb on + strand, within Pf6N2E2_143 at 195.192 kb on + strand, within Pf6N2E2_143 at 195.192 kb on + strand, within Pf6N2E2_143 at 195.193 kb on - strand, within Pf6N2E2_143 at 195.193 kb on - strand, within Pf6N2E2_143 at 195.193 kb on - strand, within Pf6N2E2_143 at 195.254 kb on - strand, within Pf6N2E2_143 at 195.254 kb on - strand, within Pf6N2E2_143 at 195.254 kb on - strand, within Pf6N2E2_143 at 195.254 kb on - strand, within Pf6N2E2_143 at 195.265 kb on + strand, within Pf6N2E2_143 at 195.266 kb on - strand, within Pf6N2E2_143 at 195.268 kb on - strand, within Pf6N2E2_143 at 195.281 kb on - strand, within Pf6N2E2_143 at 195.371 kb on - strand at 195.414 kb on + strand at 195.520 kb on + strand at 195.520 kb on + strand at 195.521 kb on - strand at 195.521 kb on - strand at 195.559 kb on + strand at 195.559 kb on + strand at 195.559 kb on + strand at 195.559 kb on + strand at 195.559 kb on + strand at 195.559 kb on + strand at 195.559 kb on + strand at 195.559 kb on + strand at 195.560 kb on - strand at 195.560 kb on - strand at 195.560 kb on - strand at 195.560 kb on - strand at 195.560 kb on - strand at 195.560 kb on - strand at 195.560 kb on - strand at 195.560 kb on - strand at 195.560 kb on - strand at 195.560 kb on - strand at 195.560 kb on - strand at 195.560 kb on - strand at 195.560 kb on - strand at 195.567 kb on + strand at 195.567 kb on + strand at 195.567 kb on + strand at 195.567 kb on + strand at 195.567 kb on + strand at 195.567 kb on + strand at 195.567 kb on + strand at 195.567 kb on + strand at 195.567 kb on + strand at 195.567 kb on + strand at 195.567 kb on + strand at 195.568 kb on - strand at 195.568 kb on - strand at 195.568 kb on - strand at 195.568 kb on - strand at 195.568 kb on - strand at 195.569 kb on + strand at 195.570 kb on - strand at 195.570 kb on - strand at 195.664 kb on - strand, within Pf6N2E2_144 at 195.666 kb on + strand, within Pf6N2E2_144 at 195.666 kb on + strand at 195.982 kb on - strand, within Pf6N2E2_144 at 195.999 kb on + strand, within Pf6N2E2_144 at 195.999 kb on + strand at 195.999 kb on + strand, within Pf6N2E2_144 at 196.000 kb on - strand, within Pf6N2E2_144 at 196.089 kb on + strand, within Pf6N2E2_144 at 196.090 kb on - strand, within Pf6N2E2_144 at 196.090 kb on - strand, within Pf6N2E2_144 at 196.141 kb on - strand, within Pf6N2E2_144 at 196.326 kb on + strand, within Pf6N2E2_144 at 196.326 kb on + strand, within Pf6N2E2_144 at 196.326 kb on + strand at 196.327 kb on - strand, within Pf6N2E2_144 at 196.327 kb on - strand, within Pf6N2E2_144 at 196.377 kb on + strand, within Pf6N2E2_144 at 196.378 kb on - strand, within Pf6N2E2_144 at 196.414 kb on + strand, within Pf6N2E2_144 at 196.414 kb on + strand, within Pf6N2E2_144 at 196.414 kb on + strand, within Pf6N2E2_144 at 196.414 kb on + strand, within Pf6N2E2_144 at 196.414 kb on + strand, within Pf6N2E2_144 at 196.414 kb on + strand, within Pf6N2E2_144 at 196.414 kb on + strand, within Pf6N2E2_144 at 196.414 kb on + strand, within Pf6N2E2_144 at 196.414 kb on + strand, within Pf6N2E2_144 at 196.414 kb on + strand, within Pf6N2E2_144 at 196.414 kb on + strand, within Pf6N2E2_144 at 196.414 kb on + strand, within Pf6N2E2_144 at 196.415 kb on - strand, within Pf6N2E2_144 at 196.415 kb on - strand, within Pf6N2E2_144 at 196.415 kb on - strand, within Pf6N2E2_144 at 196.415 kb on - strand, within Pf6N2E2_144 at 196.415 kb on - strand, within Pf6N2E2_144 at 196.415 kb on - strand, within Pf6N2E2_144 at 196.415 kb on - strand, within Pf6N2E2_144 at 196.415 kb on - strand, within Pf6N2E2_144 at 196.415 kb on - strand, within Pf6N2E2_144 at 196.415 kb on - strand, within Pf6N2E2_144 at 196.437 kb on + strand, within Pf6N2E2_144 at 196.437 kb on + strand, within Pf6N2E2_144 at 196.437 kb on + strand, within Pf6N2E2_144 at 196.437 kb on + strand, within Pf6N2E2_144 at 196.437 kb on + strand, within Pf6N2E2_144 at 196.438 kb on - strand, within Pf6N2E2_144 at 196.438 kb on - strand, within Pf6N2E2_144 at 196.438 kb on - strand, within Pf6N2E2_144 at 196.438 kb on - strand, within Pf6N2E2_144 at 196.438 kb on - strand, within Pf6N2E2_144 at 196.557 kb on + strand, within Pf6N2E2_144 at 196.611 kb on + strand, within Pf6N2E2_144 at 196.611 kb on + strand, within Pf6N2E2_144 at 196.611 kb on + strand, within Pf6N2E2_144 at 196.612 kb on - strand, within Pf6N2E2_144 at 196.612 kb on - strand, within Pf6N2E2_144 at 196.612 kb on - strand, within Pf6N2E2_144 at 196.617 kb on + strand, within Pf6N2E2_144 at 196.617 kb on + strand, within Pf6N2E2_144 at 196.617 kb on + strand, within Pf6N2E2_144 at 196.617 kb on + strand, within Pf6N2E2_144 at 196.617 kb on + strand, within Pf6N2E2_144 at 196.618 kb on - strand, within Pf6N2E2_144 at 196.618 kb on - strand, within Pf6N2E2_144 at 196.717 kb on + strand, within Pf6N2E2_144 at 196.717 kb on + strand, within Pf6N2E2_144 at 196.717 kb on + strand, within Pf6N2E2_144 at 196.718 kb on - strand, within Pf6N2E2_144 at 196.719 kb on + strand, within Pf6N2E2_144 at 196.719 kb on + strand, within Pf6N2E2_144 at 196.720 kb on - strand, within Pf6N2E2_144 at 196.720 kb on - strand, within Pf6N2E2_144 at 196.865 kb on - strand, within Pf6N2E2_144 at 196.865 kb on - strand, within Pf6N2E2_144 at 196.929 kb on + strand, within Pf6N2E2_144 at 196.929 kb on + strand, within Pf6N2E2_144 at 196.929 kb on + strand, within Pf6N2E2_144 at 196.929 kb on + strand, within Pf6N2E2_144 at 196.929 kb on + strand, within Pf6N2E2_144 at 196.929 kb on + strand, within Pf6N2E2_144 at 196.929 kb on + strand, within Pf6N2E2_144 at 196.930 kb on - strand, within Pf6N2E2_144 at 196.930 kb on - strand, within Pf6N2E2_144 at 196.930 kb on - strand, within Pf6N2E2_144 at 196.930 kb on - strand, within Pf6N2E2_144 at 196.930 kb on - strand, within Pf6N2E2_144 at 196.930 kb on - strand, within Pf6N2E2_144 at 196.930 kb on - strand, within Pf6N2E2_144 at 196.930 kb on - strand, within Pf6N2E2_144 at 196.954 kb on - strand, within Pf6N2E2_144 at 196.959 kb on + strand, within Pf6N2E2_144 at 196.959 kb on + strand, within Pf6N2E2_144 at 196.960 kb on - strand, within Pf6N2E2_144 at 196.960 kb on - strand, within Pf6N2E2_144 at 196.960 kb on - strand, within Pf6N2E2_144 at 197.015 kb on + strand at 197.015 kb on + strand at 197.016 kb on - strand at 197.016 kb on - strand at 197.181 kb on + strand at 197.182 kb on - strand at 197.182 kb on - strand at 197.376 kb on + strand at 197.822 kb on + strand, within Pf6N2E2_145 at 197.823 kb on - strand, within Pf6N2E2_145 at 197.823 kb on - strand, within Pf6N2E2_145
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 194,574 + +0.2 194,574 + -0.9 194,575 - +1.1 194,575 - +0.3 194,575 - +0.2 194,641 + -0.0 194,641 + -0.8 194,641 + +1.1 194,642 - +0.8 194,670 + +0.2 194,671 - +0.3 194,677 + +0.1 194,757 + +0.5 194,757 + -2.1 194,757 + -0.3 194,758 - +0.0 194,773 + +1.2 194,801 - Pf6N2E2_143 0.12 -0.9 194,801 - Pf6N2E2_143 0.12 -0.4 194,801 - Pf6N2E2_143 0.12 +1.0 194,801 - Pf6N2E2_143 0.12 +0.1 194,833 + Pf6N2E2_143 0.17 -1.8 194,833 + Pf6N2E2_143 0.17 -1.0 194,834 - Pf6N2E2_143 0.17 +0.5 194,834 - Pf6N2E2_143 0.17 +0.4 194,834 - Pf6N2E2_143 0.17 +0.3 194,834 - Pf6N2E2_143 0.17 -0.4 195,074 - Pf6N2E2_143 0.54 +0.8 195,074 - Pf6N2E2_143 0.54 +0.5 195,119 + Pf6N2E2_143 0.61 -0.4 195,119 + Pf6N2E2_143 0.61 +1.7 195,119 + Pf6N2E2_143 0.61 -0.0 195,119 + Pf6N2E2_143 0.61 +0.2 195,119 + Pf6N2E2_143 0.61 -0.5 195,120 - Pf6N2E2_143 0.61 +1.6 195,120 - Pf6N2E2_143 0.61 +0.5 195,120 - Pf6N2E2_143 0.61 +0.3 195,192 + Pf6N2E2_143 0.72 -0.7 195,192 + Pf6N2E2_143 0.72 +0.3 195,192 + Pf6N2E2_143 0.72 -0.1 195,192 + Pf6N2E2_143 0.72 +1.0 195,192 + Pf6N2E2_143 0.72 -0.0 195,192 + Pf6N2E2_143 0.72 -0.7 195,193 - Pf6N2E2_143 0.72 +1.1 195,193 - Pf6N2E2_143 0.72 +0.5 195,193 - Pf6N2E2_143 0.72 +0.5 195,254 - Pf6N2E2_143 0.82 +0.4 195,254 - Pf6N2E2_143 0.82 -0.5 195,254 - Pf6N2E2_143 0.82 +1.4 195,254 - Pf6N2E2_143 0.82 +0.1 195,265 + Pf6N2E2_143 0.83 +0.1 195,266 - Pf6N2E2_143 0.84 -1.9 195,268 - Pf6N2E2_143 0.84 +0.6 195,281 - Pf6N2E2_143 0.86 +1.3 195,371 - +0.8 195,414 + -1.6 195,520 + -0.9 195,520 + -1.1 195,521 - +0.0 195,521 - -1.5 195,559 + -0.7 195,559 + -0.9 195,559 + -0.7 195,559 + -1.6 195,559 + -0.3 195,559 + +0.3 195,559 + -0.8 195,559 + -1.8 195,560 - -1.3 195,560 - -2.6 195,560 - -0.6 195,560 - -0.7 195,560 - -0.0 195,560 - -1.7 195,560 - -1.1 195,560 - -1.5 195,560 - -1.3 195,560 - -1.8 195,560 - -1.2 195,560 - -1.3 195,560 - -0.5 195,567 + -3.3 195,567 + -0.6 195,567 + -1.4 195,567 + -0.7 195,567 + -0.3 195,567 + -1.7 195,567 + -1.0 195,567 + -1.3 195,567 + -1.9 195,567 + -0.9 195,567 + -1.4 195,568 - -0.8 195,568 - -0.4 195,568 - -0.6 195,568 - -1.8 195,568 - -1.6 195,569 + -0.9 195,570 - +0.1 195,570 - -1.6 195,664 - Pf6N2E2_144 0.12 -0.9 195,666 + Pf6N2E2_144 0.12 -0.7 195,666 + +1.0 195,982 - Pf6N2E2_144 0.31 +1.0 195,999 + Pf6N2E2_144 0.32 -1.0 195,999 + +1.0 195,999 + Pf6N2E2_144 0.32 -3.8 196,000 - Pf6N2E2_144 0.32 -1.3 196,089 + Pf6N2E2_144 0.37 -1.1 196,090 - Pf6N2E2_144 0.37 -1.1 196,090 - Pf6N2E2_144 0.37 -1.2 196,141 - Pf6N2E2_144 0.40 -0.6 196,326 + Pf6N2E2_144 0.51 -1.8 196,326 + Pf6N2E2_144 0.51 -2.0 196,326 + +1.0 196,327 - Pf6N2E2_144 0.51 -0.9 196,327 - Pf6N2E2_144 0.51 -0.4 196,377 + Pf6N2E2_144 0.54 -2.3 196,378 - Pf6N2E2_144 0.54 -1.0 196,414 + Pf6N2E2_144 0.56 -1.2 196,414 + Pf6N2E2_144 0.56 -1.7 196,414 + Pf6N2E2_144 0.56 -1.0 196,414 + Pf6N2E2_144 0.56 -0.2 196,414 + Pf6N2E2_144 0.56 -1.1 196,414 + Pf6N2E2_144 0.56 +0.5 196,414 + Pf6N2E2_144 0.56 -0.2 196,414 + Pf6N2E2_144 0.56 -1.2 196,414 + Pf6N2E2_144 0.56 -1.8 196,414 + Pf6N2E2_144 0.56 -1.3 196,414 + Pf6N2E2_144 0.56 -1.5 196,414 + Pf6N2E2_144 0.56 -2.3 196,415 - Pf6N2E2_144 0.56 -1.2 196,415 - Pf6N2E2_144 0.56 -1.9 196,415 - Pf6N2E2_144 0.56 -0.8 196,415 - Pf6N2E2_144 0.56 -1.3 196,415 - Pf6N2E2_144 0.56 -2.3 196,415 - Pf6N2E2_144 0.56 -1.4 196,415 - Pf6N2E2_144 0.56 -0.7 196,415 - Pf6N2E2_144 0.56 -0.1 196,415 - Pf6N2E2_144 0.56 -3.3 196,415 - Pf6N2E2_144 0.56 -1.0 196,437 + Pf6N2E2_144 0.57 -0.3 196,437 + Pf6N2E2_144 0.57 -1.4 196,437 + Pf6N2E2_144 0.57 +1.0 196,437 + Pf6N2E2_144 0.57 -0.3 196,437 + Pf6N2E2_144 0.57 -1.3 196,438 - Pf6N2E2_144 0.57 -0.8 196,438 - Pf6N2E2_144 0.57 -1.8 196,438 - Pf6N2E2_144 0.57 -0.7 196,438 - Pf6N2E2_144 0.57 -0.2 196,438 - Pf6N2E2_144 0.57 -1.2 196,557 + Pf6N2E2_144 0.64 -2.6 196,611 + Pf6N2E2_144 0.68 -2.1 196,611 + Pf6N2E2_144 0.68 -1.5 196,611 + Pf6N2E2_144 0.68 -2.2 196,612 - Pf6N2E2_144 0.68 -0.7 196,612 - Pf6N2E2_144 0.68 -1.1 196,612 - Pf6N2E2_144 0.68 +0.1 196,617 + Pf6N2E2_144 0.68 +1.0 196,617 + Pf6N2E2_144 0.68 -2.0 196,617 + Pf6N2E2_144 0.68 +0.6 196,617 + Pf6N2E2_144 0.68 -1.8 196,617 + Pf6N2E2_144 0.68 -2.8 196,618 - Pf6N2E2_144 0.68 -1.8 196,618 - Pf6N2E2_144 0.68 -0.5 196,717 + Pf6N2E2_144 0.74 -2.3 196,717 + Pf6N2E2_144 0.74 -0.9 196,717 + Pf6N2E2_144 0.74 -1.1 196,718 - Pf6N2E2_144 0.74 -0.0 196,719 + Pf6N2E2_144 0.74 -1.4 196,719 + Pf6N2E2_144 0.74 -2.2 196,720 - Pf6N2E2_144 0.74 -1.2 196,720 - Pf6N2E2_144 0.74 -1.5 196,865 - Pf6N2E2_144 0.82 -0.9 196,865 - Pf6N2E2_144 0.82 -0.8 196,929 + Pf6N2E2_144 0.86 -0.7 196,929 + Pf6N2E2_144 0.86 -0.2 196,929 + Pf6N2E2_144 0.86 -1.9 196,929 + Pf6N2E2_144 0.86 -0.7 196,929 + Pf6N2E2_144 0.86 -1.2 196,929 + Pf6N2E2_144 0.86 -2.0 196,929 + Pf6N2E2_144 0.86 -0.5 196,930 - Pf6N2E2_144 0.86 +0.4 196,930 - Pf6N2E2_144 0.86 -0.6 196,930 - Pf6N2E2_144 0.86 -1.8 196,930 - Pf6N2E2_144 0.86 -2.4 196,930 - Pf6N2E2_144 0.86 -0.3 196,930 - Pf6N2E2_144 0.86 -0.6 196,930 - Pf6N2E2_144 0.86 -1.3 196,930 - Pf6N2E2_144 0.86 -3.7 196,954 - Pf6N2E2_144 0.88 -1.1 196,959 + Pf6N2E2_144 0.88 -0.3 196,959 + Pf6N2E2_144 0.88 -0.2 196,960 - Pf6N2E2_144 0.88 -0.0 196,960 - Pf6N2E2_144 0.88 -1.4 196,960 - Pf6N2E2_144 0.88 -1.6 197,015 + -1.4 197,015 + -0.3 197,016 - -0.3 197,016 - -0.0 197,181 + +0.5 197,182 - -1.8 197,182 - -1.4 197,376 + -1.7 197,822 + Pf6N2E2_145 0.17 -0.6 197,823 - Pf6N2E2_145 0.18 -0.9 197,823 - Pf6N2E2_145 0.18 -3.0
Or see this region's nucleotide sequence