Experiment: Tetracycline 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1084 and Pf6N2E2_1085 are separated by 9 nucleotides Pf6N2E2_1085 and Pf6N2E2_1086 are separated by 295 nucleotides Pf6N2E2_1086 and Pf6N2E2_1087 are separated by 221 nucleotides
Pf6N2E2_1084 - hypothetical protein, at 1,266,931 to 1,267,119
_1084
Pf6N2E2_1085 - Fe2+/Zn2+ uptake regulation proteins, at 1,267,129 to 1,267,509
_1085
Pf6N2E2_1086 - Acyl-homoserine lactone acylase PvdQ (EC 3.5.1.-), quorum-quenching, at 1,267,805 to 1,270,153
_1086
Pf6N2E2_1087 - Iron siderophore sensor protein, at 1,270,375 to 1,271,376
_1087
Position (kb)
1267
1268
1269
1270
1271 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1266.819 kb on + strand at 1266.820 kb on - strand at 1266.835 kb on + strand at 1266.874 kb on - strand at 1266.912 kb on + strand at 1266.913 kb on - strand at 1266.929 kb on + strand at 1267.048 kb on + strand, within Pf6N2E2_1084 at 1267.048 kb on + strand, within Pf6N2E2_1084 at 1267.435 kb on + strand, within Pf6N2E2_1085 at 1267.435 kb on + strand, within Pf6N2E2_1085 at 1267.436 kb on - strand, within Pf6N2E2_1085 at 1267.560 kb on + strand at 1267.561 kb on - strand at 1267.563 kb on + strand at 1267.564 kb on - strand at 1267.564 kb on - strand at 1267.658 kb on - strand at 1267.994 kb on + strand at 1268.214 kb on - strand, within Pf6N2E2_1086 at 1268.699 kb on + strand, within Pf6N2E2_1086 at 1268.699 kb on + strand, within Pf6N2E2_1086 at 1268.719 kb on + strand, within Pf6N2E2_1086 at 1268.834 kb on + strand at 1268.835 kb on - strand, within Pf6N2E2_1086 at 1269.062 kb on + strand, within Pf6N2E2_1086 at 1269.063 kb on - strand, within Pf6N2E2_1086 at 1269.063 kb on - strand, within Pf6N2E2_1086 at 1269.212 kb on + strand, within Pf6N2E2_1086 at 1269.213 kb on - strand, within Pf6N2E2_1086 at 1269.213 kb on - strand, within Pf6N2E2_1086 at 1269.251 kb on + strand, within Pf6N2E2_1086 at 1269.252 kb on - strand, within Pf6N2E2_1086 at 1269.252 kb on - strand, within Pf6N2E2_1086 at 1269.860 kb on + strand, within Pf6N2E2_1086 at 1269.861 kb on - strand, within Pf6N2E2_1086 at 1269.923 kb on + strand at 1270.097 kb on + strand at 1270.098 kb on - strand at 1270.098 kb on - strand at 1270.151 kb on + strand at 1270.186 kb on + strand at 1270.186 kb on + strand at 1270.187 kb on - strand at 1270.187 kb on - strand at 1270.229 kb on + strand at 1270.229 kb on + strand at 1270.229 kb on + strand at 1270.229 kb on + strand at 1270.229 kb on + strand at 1270.229 kb on + strand at 1270.229 kb on + strand at 1270.230 kb on - strand at 1270.352 kb on + strand at 1270.353 kb on - strand at 1270.353 kb on - strand at 1270.353 kb on - strand at 1270.443 kb on - strand at 1270.499 kb on + strand, within Pf6N2E2_1087 at 1270.500 kb on - strand, within Pf6N2E2_1087 at 1270.500 kb on - strand, within Pf6N2E2_1087 at 1270.691 kb on + strand, within Pf6N2E2_1087 at 1270.692 kb on - strand, within Pf6N2E2_1087 at 1270.692 kb on - strand, within Pf6N2E2_1087 at 1270.775 kb on - strand, within Pf6N2E2_1087 at 1270.799 kb on + strand, within Pf6N2E2_1087 at 1270.799 kb on + strand, within Pf6N2E2_1087 at 1270.800 kb on - strand, within Pf6N2E2_1087 at 1270.800 kb on - strand, within Pf6N2E2_1087 at 1270.807 kb on + strand, within Pf6N2E2_1087 at 1270.807 kb on + strand, within Pf6N2E2_1087 at 1270.807 kb on + strand, within Pf6N2E2_1087 at 1270.807 kb on + strand, within Pf6N2E2_1087 at 1270.807 kb on + strand, within Pf6N2E2_1087 at 1270.808 kb on - strand, within Pf6N2E2_1087 at 1270.808 kb on - strand, within Pf6N2E2_1087 at 1270.938 kb on - strand, within Pf6N2E2_1087 at 1271.001 kb on + strand, within Pf6N2E2_1087 at 1271.001 kb on + strand, within Pf6N2E2_1087 at 1271.002 kb on - strand, within Pf6N2E2_1087 at 1271.002 kb on - strand, within Pf6N2E2_1087 at 1271.002 kb on - strand, within Pf6N2E2_1087 at 1271.008 kb on + strand, within Pf6N2E2_1087 at 1271.036 kb on + strand, within Pf6N2E2_1087 at 1271.037 kb on - strand, within Pf6N2E2_1087 at 1271.073 kb on - strand, within Pf6N2E2_1087 at 1271.073 kb on - strand, within Pf6N2E2_1087 at 1271.095 kb on + strand, within Pf6N2E2_1087 at 1271.099 kb on - strand, within Pf6N2E2_1087 at 1271.099 kb on - strand, within Pf6N2E2_1087 at 1271.099 kb on - strand, within Pf6N2E2_1087
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0004 mg/ml remove 1,266,819 + +0.1 1,266,820 - +0.6 1,266,835 + -1.5 1,266,874 - -0.7 1,266,912 + -0.6 1,266,913 - -0.6 1,266,929 + -1.2 1,267,048 + Pf6N2E2_1084 0.62 -0.6 1,267,048 + Pf6N2E2_1084 0.62 -0.1 1,267,435 + Pf6N2E2_1085 0.80 -0.6 1,267,435 + Pf6N2E2_1085 0.80 +0.8 1,267,436 - Pf6N2E2_1085 0.81 -2.5 1,267,560 + +2.3 1,267,561 - -0.2 1,267,563 + -0.3 1,267,564 - -1.6 1,267,564 - +0.5 1,267,658 - +0.5 1,267,994 + -0.3 1,268,214 - Pf6N2E2_1086 0.17 +1.2 1,268,699 + Pf6N2E2_1086 0.38 +0.3 1,268,699 + Pf6N2E2_1086 0.38 +1.4 1,268,719 + Pf6N2E2_1086 0.39 -0.2 1,268,834 + +0.7 1,268,835 - Pf6N2E2_1086 0.44 +2.3 1,269,062 + Pf6N2E2_1086 0.54 +0.3 1,269,063 - Pf6N2E2_1086 0.54 -0.4 1,269,063 - Pf6N2E2_1086 0.54 +0.3 1,269,212 + Pf6N2E2_1086 0.60 +0.8 1,269,213 - Pf6N2E2_1086 0.60 +0.7 1,269,213 - Pf6N2E2_1086 0.60 -1.7 1,269,251 + Pf6N2E2_1086 0.62 -0.0 1,269,252 - Pf6N2E2_1086 0.62 -0.4 1,269,252 - Pf6N2E2_1086 0.62 -0.9 1,269,860 + Pf6N2E2_1086 0.87 -1.1 1,269,861 - Pf6N2E2_1086 0.88 +2.0 1,269,923 + +1.2 1,270,097 + +0.6 1,270,098 - +0.7 1,270,098 - -0.5 1,270,151 + -1.6 1,270,186 + -0.3 1,270,186 + -0.1 1,270,187 - +1.3 1,270,187 - +1.1 1,270,229 + -0.3 1,270,229 + -0.5 1,270,229 + +0.1 1,270,229 + -0.6 1,270,229 + +1.7 1,270,229 + -0.5 1,270,229 + +0.4 1,270,230 - -0.5 1,270,352 + -2.5 1,270,353 - -0.7 1,270,353 - -0.4 1,270,353 - -0.0 1,270,443 - -1.8 1,270,499 + Pf6N2E2_1087 0.12 -1.6 1,270,500 - Pf6N2E2_1087 0.12 -0.5 1,270,500 - Pf6N2E2_1087 0.12 +0.4 1,270,691 + Pf6N2E2_1087 0.32 +0.1 1,270,692 - Pf6N2E2_1087 0.32 +1.0 1,270,692 - Pf6N2E2_1087 0.32 +0.1 1,270,775 - Pf6N2E2_1087 0.40 +0.0 1,270,799 + Pf6N2E2_1087 0.42 +1.1 1,270,799 + Pf6N2E2_1087 0.42 +0.5 1,270,800 - Pf6N2E2_1087 0.42 +0.4 1,270,800 - Pf6N2E2_1087 0.42 +0.1 1,270,807 + Pf6N2E2_1087 0.43 +0.5 1,270,807 + Pf6N2E2_1087 0.43 +0.3 1,270,807 + Pf6N2E2_1087 0.43 +0.3 1,270,807 + Pf6N2E2_1087 0.43 -0.0 1,270,807 + Pf6N2E2_1087 0.43 -0.5 1,270,808 - Pf6N2E2_1087 0.43 -1.5 1,270,808 - Pf6N2E2_1087 0.43 -1.2 1,270,938 - Pf6N2E2_1087 0.56 -1.2 1,271,001 + Pf6N2E2_1087 0.62 -0.4 1,271,001 + Pf6N2E2_1087 0.62 +0.4 1,271,002 - Pf6N2E2_1087 0.63 -0.9 1,271,002 - Pf6N2E2_1087 0.63 +1.1 1,271,002 - Pf6N2E2_1087 0.63 -0.5 1,271,008 + Pf6N2E2_1087 0.63 -1.2 1,271,036 + Pf6N2E2_1087 0.66 -1.6 1,271,037 - Pf6N2E2_1087 0.66 -1.3 1,271,073 - Pf6N2E2_1087 0.70 +0.1 1,271,073 - Pf6N2E2_1087 0.70 -0.9 1,271,095 + Pf6N2E2_1087 0.72 +0.1 1,271,099 - Pf6N2E2_1087 0.72 +0.7 1,271,099 - Pf6N2E2_1087 0.72 -1.4 1,271,099 - Pf6N2E2_1087 0.72 -0.4
Or see this region's nucleotide sequence