Experiment: Tetracycline 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5897 and Pf6N2E2_5898 are separated by 277 nucleotides Pf6N2E2_5898 and Pf6N2E2_5899 are separated by 404 nucleotides Pf6N2E2_5899 and Pf6N2E2_5900 are separated by 73 nucleotides
Pf6N2E2_5897 - COG1180: Radical SAM, Pyruvate-formate lyase-activating enzyme like, at 6,717,754 to 6,719,064
_5897
Pf6N2E2_5898 - Integral membrane protein, at 6,719,342 to 6,719,950
_5898
Pf6N2E2_5899 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 6,720,355 to 6,720,675
_5899
Pf6N2E2_5900 - L-Proline/Glycine betaine transporter ProP, at 6,720,749 to 6,721,999
_5900
Position (kb)
6719
6720 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6718.349 kb on + strand, within Pf6N2E2_5897 at 6718.349 kb on + strand, within Pf6N2E2_5897 at 6718.350 kb on - strand, within Pf6N2E2_5897 at 6718.350 kb on - strand, within Pf6N2E2_5897 at 6718.568 kb on + strand, within Pf6N2E2_5897 at 6718.568 kb on + strand, within Pf6N2E2_5897 at 6718.569 kb on - strand, within Pf6N2E2_5897 at 6718.643 kb on + strand, within Pf6N2E2_5897 at 6718.644 kb on - strand, within Pf6N2E2_5897 at 6718.657 kb on - strand, within Pf6N2E2_5897 at 6718.657 kb on - strand, within Pf6N2E2_5897 at 6718.681 kb on - strand, within Pf6N2E2_5897 at 6718.741 kb on + strand, within Pf6N2E2_5897 at 6718.742 kb on - strand, within Pf6N2E2_5897 at 6718.742 kb on - strand, within Pf6N2E2_5897 at 6718.742 kb on - strand, within Pf6N2E2_5897 at 6718.817 kb on + strand, within Pf6N2E2_5897 at 6718.817 kb on + strand, within Pf6N2E2_5897 at 6718.818 kb on - strand, within Pf6N2E2_5897 at 6718.818 kb on - strand, within Pf6N2E2_5897 at 6718.884 kb on - strand, within Pf6N2E2_5897 at 6718.884 kb on - strand, within Pf6N2E2_5897 at 6719.126 kb on - strand at 6719.373 kb on + strand at 6719.631 kb on - strand, within Pf6N2E2_5898 at 6719.631 kb on - strand, within Pf6N2E2_5898 at 6719.652 kb on - strand, within Pf6N2E2_5898 at 6719.658 kb on + strand, within Pf6N2E2_5898 at 6719.659 kb on - strand, within Pf6N2E2_5898 at 6719.754 kb on - strand, within Pf6N2E2_5898 at 6719.839 kb on + strand, within Pf6N2E2_5898 at 6719.947 kb on - strand at 6720.254 kb on + strand at 6720.333 kb on + strand at 6720.404 kb on + strand, within Pf6N2E2_5899 at 6720.404 kb on + strand, within Pf6N2E2_5899 at 6720.432 kb on - strand, within Pf6N2E2_5899 at 6720.479 kb on + strand, within Pf6N2E2_5899 at 6720.480 kb on - strand, within Pf6N2E2_5899 at 6720.482 kb on + strand, within Pf6N2E2_5899 at 6720.482 kb on + strand, within Pf6N2E2_5899 at 6720.500 kb on + strand, within Pf6N2E2_5899 at 6720.500 kb on + strand, within Pf6N2E2_5899 at 6720.500 kb on + strand, within Pf6N2E2_5899 at 6720.500 kb on + strand, within Pf6N2E2_5899 at 6720.500 kb on + strand, within Pf6N2E2_5899 at 6720.500 kb on + strand, within Pf6N2E2_5899 at 6720.501 kb on - strand, within Pf6N2E2_5899 at 6720.501 kb on - strand, within Pf6N2E2_5899 at 6720.544 kb on + strand, within Pf6N2E2_5899 at 6720.544 kb on + strand, within Pf6N2E2_5899 at 6720.624 kb on + strand, within Pf6N2E2_5899 at 6720.625 kb on - strand, within Pf6N2E2_5899 at 6720.751 kb on - strand at 6720.804 kb on - strand at 6720.804 kb on - strand at 6720.804 kb on - strand at 6720.806 kb on + strand at 6720.806 kb on + strand at 6720.807 kb on - strand at 6720.807 kb on - strand at 6720.807 kb on - strand at 6720.807 kb on - strand at 6720.807 kb on - strand at 6720.874 kb on + strand, within Pf6N2E2_5900 at 6720.930 kb on - strand, within Pf6N2E2_5900
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0002 mg/ml remove 6,718,349 + Pf6N2E2_5897 0.45 +0.3 6,718,349 + Pf6N2E2_5897 0.45 +0.5 6,718,350 - Pf6N2E2_5897 0.45 -0.6 6,718,350 - Pf6N2E2_5897 0.45 +0.5 6,718,568 + Pf6N2E2_5897 0.62 -1.2 6,718,568 + Pf6N2E2_5897 0.62 +1.0 6,718,569 - Pf6N2E2_5897 0.62 +0.7 6,718,643 + Pf6N2E2_5897 0.68 -0.2 6,718,644 - Pf6N2E2_5897 0.68 -0.7 6,718,657 - Pf6N2E2_5897 0.69 +0.5 6,718,657 - Pf6N2E2_5897 0.69 -0.1 6,718,681 - Pf6N2E2_5897 0.71 -0.7 6,718,741 + Pf6N2E2_5897 0.75 -0.5 6,718,742 - Pf6N2E2_5897 0.75 +0.2 6,718,742 - Pf6N2E2_5897 0.75 +0.5 6,718,742 - Pf6N2E2_5897 0.75 -0.9 6,718,817 + Pf6N2E2_5897 0.81 -0.2 6,718,817 + Pf6N2E2_5897 0.81 -1.5 6,718,818 - Pf6N2E2_5897 0.81 +0.4 6,718,818 - Pf6N2E2_5897 0.81 +0.5 6,718,884 - Pf6N2E2_5897 0.86 +0.6 6,718,884 - Pf6N2E2_5897 0.86 -0.6 6,719,126 - -0.7 6,719,373 + -0.6 6,719,631 - Pf6N2E2_5898 0.47 -1.3 6,719,631 - Pf6N2E2_5898 0.47 -1.7 6,719,652 - Pf6N2E2_5898 0.51 -0.3 6,719,658 + Pf6N2E2_5898 0.52 +0.8 6,719,659 - Pf6N2E2_5898 0.52 -0.2 6,719,754 - Pf6N2E2_5898 0.68 -0.5 6,719,839 + Pf6N2E2_5898 0.82 -0.5 6,719,947 - +0.0 6,720,254 + +0.1 6,720,333 + -0.8 6,720,404 + Pf6N2E2_5899 0.15 +0.8 6,720,404 + Pf6N2E2_5899 0.15 +0.3 6,720,432 - Pf6N2E2_5899 0.24 -0.9 6,720,479 + Pf6N2E2_5899 0.39 -0.7 6,720,480 - Pf6N2E2_5899 0.39 +0.1 6,720,482 + Pf6N2E2_5899 0.40 +0.7 6,720,482 + Pf6N2E2_5899 0.40 -0.2 6,720,500 + Pf6N2E2_5899 0.45 +0.1 6,720,500 + Pf6N2E2_5899 0.45 +0.8 6,720,500 + Pf6N2E2_5899 0.45 +0.2 6,720,500 + Pf6N2E2_5899 0.45 +1.0 6,720,500 + Pf6N2E2_5899 0.45 +0.7 6,720,500 + Pf6N2E2_5899 0.45 +0.3 6,720,501 - Pf6N2E2_5899 0.45 +1.0 6,720,501 - Pf6N2E2_5899 0.45 -1.1 6,720,544 + Pf6N2E2_5899 0.59 +0.6 6,720,544 + Pf6N2E2_5899 0.59 -1.5 6,720,624 + Pf6N2E2_5899 0.84 -0.4 6,720,625 - Pf6N2E2_5899 0.84 +1.0 6,720,751 - -0.2 6,720,804 - +1.1 6,720,804 - -3.0 6,720,804 - +0.7 6,720,806 + +0.4 6,720,806 + -0.1 6,720,807 - +0.9 6,720,807 - +0.1 6,720,807 - +0.2 6,720,807 - +0.8 6,720,807 - -0.4 6,720,874 + Pf6N2E2_5900 0.10 -0.4 6,720,930 - Pf6N2E2_5900 0.14 +2.2
Or see this region's nucleotide sequence