Experiment: Tetracycline 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_565 and Pf6N2E2_566 are separated by 165 nucleotides Pf6N2E2_566 and Pf6N2E2_567 are separated by 185 nucleotides Pf6N2E2_567 and Pf6N2E2_568 are separated by 178 nucleotides Pf6N2E2_568 and Pf6N2E2_569 are separated by 197 nucleotides
Pf6N2E2_565 - hypothetical protein, at 641,187 to 641,402
_565
Pf6N2E2_566 - hypothetical protein, at 641,568 to 642,038
_566
Pf6N2E2_567 - Universal stress protein family 7, at 642,224 to 643,087
_567
Pf6N2E2_568 - tRNA-(ms[2]io[6]A)-hydroxylase (EC 1.-.-.-), at 643,266 to 643,871
_568
Pf6N2E2_569 - FIG00793915: hypothetical protein, at 644,069 to 644,557
_569
Position (kb) 642
643
644 Strain fitness (log2 ratio) -1
0
1
2 at 641.422 kb on - strand at 641.422 kb on - strand at 641.440 kb on - strand at 641.440 kb on - strand at 641.480 kb on - strand at 641.997 kb on + strand at 641.997 kb on + strand at 641.997 kb on + strand at 641.997 kb on + strand at 641.998 kb on - strand at 642.001 kb on + strand at 642.002 kb on - strand at 642.329 kb on + strand, within Pf6N2E2_567 at 642.330 kb on - strand, within Pf6N2E2_567 at 642.330 kb on - strand, within Pf6N2E2_567 at 642.405 kb on + strand, within Pf6N2E2_567 at 642.405 kb on + strand, within Pf6N2E2_567 at 642.405 kb on + strand, within Pf6N2E2_567 at 642.405 kb on + strand, within Pf6N2E2_567 at 642.405 kb on + strand, within Pf6N2E2_567 at 642.405 kb on + strand, within Pf6N2E2_567 at 642.405 kb on + strand, within Pf6N2E2_567 at 642.405 kb on + strand, within Pf6N2E2_567 at 642.406 kb on - strand, within Pf6N2E2_567 at 642.406 kb on - strand, within Pf6N2E2_567 at 642.406 kb on - strand, within Pf6N2E2_567 at 643.130 kb on + strand at 643.130 kb on + strand at 643.130 kb on + strand at 643.130 kb on + strand at 643.130 kb on + strand at 643.131 kb on - strand at 643.132 kb on + strand at 643.132 kb on + strand at 643.132 kb on + strand at 643.132 kb on + strand at 643.133 kb on - strand at 643.133 kb on - strand at 643.133 kb on - strand at 643.133 kb on - strand at 643.151 kb on + strand at 643.151 kb on + strand at 643.152 kb on - strand at 643.185 kb on - strand at 643.223 kb on - strand at 643.223 kb on - strand at 643.224 kb on + strand at 643.225 kb on - strand at 643.247 kb on - strand at 643.247 kb on - strand at 643.416 kb on + strand, within Pf6N2E2_568 at 643.416 kb on + strand, within Pf6N2E2_568 at 643.417 kb on - strand, within Pf6N2E2_568 at 643.417 kb on - strand, within Pf6N2E2_568 at 643.417 kb on - strand, within Pf6N2E2_568 at 643.546 kb on - strand, within Pf6N2E2_568 at 643.570 kb on + strand, within Pf6N2E2_568 at 643.571 kb on - strand, within Pf6N2E2_568 at 643.693 kb on - strand, within Pf6N2E2_568 at 643.734 kb on + strand, within Pf6N2E2_568 at 643.734 kb on + strand, within Pf6N2E2_568 at 643.870 kb on - strand at 643.882 kb on + strand at 643.883 kb on - strand at 643.895 kb on + strand at 643.921 kb on + strand at 643.921 kb on + strand at 643.921 kb on + strand at 643.921 kb on + strand at 643.922 kb on - strand at 643.947 kb on + strand at 643.947 kb on + strand at 644.053 kb on - strand at 644.070 kb on + strand at 644.070 kb on + strand at 644.070 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0002 mg/ml remove 641,422 - +0.1 641,422 - +0.6 641,440 - -0.0 641,440 - -0.1 641,480 - -0.0 641,997 + -1.2 641,997 + +0.2 641,997 + +0.6 641,997 + -1.0 641,998 - +0.4 642,001 + +0.9 642,002 - -1.2 642,329 + Pf6N2E2_567 0.12 -1.1 642,330 - Pf6N2E2_567 0.12 +0.8 642,330 - Pf6N2E2_567 0.12 -0.4 642,405 + Pf6N2E2_567 0.21 +0.2 642,405 + Pf6N2E2_567 0.21 +1.3 642,405 + Pf6N2E2_567 0.21 -0.8 642,405 + Pf6N2E2_567 0.21 +0.2 642,405 + Pf6N2E2_567 0.21 -0.9 642,405 + Pf6N2E2_567 0.21 -1.2 642,405 + Pf6N2E2_567 0.21 -1.1 642,405 + Pf6N2E2_567 0.21 +0.3 642,406 - Pf6N2E2_567 0.21 -0.5 642,406 - Pf6N2E2_567 0.21 -1.0 642,406 - Pf6N2E2_567 0.21 -0.8 643,130 + -0.4 643,130 + -0.3 643,130 + -0.1 643,130 + +0.5 643,130 + +0.4 643,131 - -0.1 643,132 + +0.3 643,132 + +0.8 643,132 + -1.0 643,132 + -0.4 643,133 - -0.0 643,133 - -0.3 643,133 - -0.6 643,133 - +0.3 643,151 + -0.1 643,151 + +0.3 643,152 - -0.4 643,185 - -0.7 643,223 - +0.6 643,223 - +0.4 643,224 + -0.8 643,225 - +0.2 643,247 - -0.9 643,247 - +0.6 643,416 + Pf6N2E2_568 0.25 -0.0 643,416 + Pf6N2E2_568 0.25 +0.2 643,417 - Pf6N2E2_568 0.25 +0.3 643,417 - Pf6N2E2_568 0.25 -1.0 643,417 - Pf6N2E2_568 0.25 -0.4 643,546 - Pf6N2E2_568 0.46 -0.2 643,570 + Pf6N2E2_568 0.50 -0.4 643,571 - Pf6N2E2_568 0.50 -0.1 643,693 - Pf6N2E2_568 0.70 -0.5 643,734 + Pf6N2E2_568 0.77 -0.8 643,734 + Pf6N2E2_568 0.77 +0.1 643,870 - +0.8 643,882 + -0.6 643,883 - -0.4 643,895 + -0.8 643,921 + -0.9 643,921 + +0.5 643,921 + +0.4 643,921 + +0.9 643,922 - +0.9 643,947 + +1.3 643,947 + +0.6 644,053 - -1.4 644,070 + -0.5 644,070 + +1.8 644,070 + -1.4
Or see this region's nucleotide sequence