Experiment: Tetracycline 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3544 and Pf6N2E2_3545 overlap by 4 nucleotides Pf6N2E2_3545 and Pf6N2E2_3546 overlap by 4 nucleotides Pf6N2E2_3546 and Pf6N2E2_3547 are separated by 381 nucleotides
Pf6N2E2_3544 - ABC transporter (iron.B12.siderophore.hemin) , ATP-binding component, at 4,135,082 to 4,135,870
_3544
Pf6N2E2_3545 - ABC transporter (iron.B12.siderophore.hemin) , periplasmic substrate-binding component, at 4,135,867 to 4,136,814
_3545
Pf6N2E2_3546 - ABC transporter (iron.B12.siderophore.hemin) , permease component, at 4,136,811 to 4,137,821
_3546
Pf6N2E2_3547 - Urea ABC transporter, substrate binding protein UrtA, at 4,138,203 to 4,139,468
_3547
Position (kb)
4136
4137
4138 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4136.069 kb on - strand, within Pf6N2E2_3545 at 4136.150 kb on - strand, within Pf6N2E2_3545 at 4136.150 kb on - strand, within Pf6N2E2_3545 at 4136.272 kb on + strand, within Pf6N2E2_3545 at 4136.331 kb on + strand, within Pf6N2E2_3545 at 4136.332 kb on - strand, within Pf6N2E2_3545 at 4136.335 kb on + strand, within Pf6N2E2_3545 at 4136.335 kb on + strand, within Pf6N2E2_3545 at 4136.335 kb on + strand, within Pf6N2E2_3545 at 4136.336 kb on - strand, within Pf6N2E2_3545 at 4136.336 kb on - strand, within Pf6N2E2_3545 at 4136.336 kb on - strand, within Pf6N2E2_3545 at 4136.336 kb on - strand, within Pf6N2E2_3545 at 4136.336 kb on - strand, within Pf6N2E2_3545 at 4136.336 kb on - strand, within Pf6N2E2_3545 at 4136.550 kb on + strand, within Pf6N2E2_3545 at 4136.600 kb on + strand, within Pf6N2E2_3545 at 4136.600 kb on + strand, within Pf6N2E2_3545 at 4136.601 kb on - strand, within Pf6N2E2_3545 at 4136.740 kb on + strand at 4136.741 kb on - strand at 4136.965 kb on - strand, within Pf6N2E2_3546 at 4137.334 kb on + strand, within Pf6N2E2_3546 at 4137.335 kb on - strand, within Pf6N2E2_3546 at 4137.335 kb on - strand, within Pf6N2E2_3546 at 4137.759 kb on - strand at 4137.759 kb on - strand at 4137.977 kb on + strand at 4137.978 kb on - strand at 4137.978 kb on - strand at 4138.182 kb on + strand at 4138.182 kb on + strand at 4138.182 kb on + strand at 4138.214 kb on + strand at 4138.214 kb on + strand at 4138.215 kb on - strand at 4138.215 kb on - strand at 4138.232 kb on + strand at 4138.232 kb on + strand at 4138.233 kb on - strand at 4138.593 kb on + strand, within Pf6N2E2_3547 at 4138.594 kb on - strand, within Pf6N2E2_3547 at 4138.627 kb on - strand, within Pf6N2E2_3547 at 4138.627 kb on - strand, within Pf6N2E2_3547 at 4138.671 kb on + strand, within Pf6N2E2_3547 at 4138.671 kb on + strand, within Pf6N2E2_3547 at 4138.671 kb on + strand, within Pf6N2E2_3547 at 4138.707 kb on + strand, within Pf6N2E2_3547 at 4138.728 kb on + strand, within Pf6N2E2_3547 at 4138.728 kb on + strand, within Pf6N2E2_3547 at 4138.729 kb on - strand, within Pf6N2E2_3547 at 4138.729 kb on - strand, within Pf6N2E2_3547 at 4138.729 kb on - strand, within Pf6N2E2_3547 at 4138.735 kb on - strand, within Pf6N2E2_3547 at 4138.812 kb on + strand, within Pf6N2E2_3547 at 4138.813 kb on - strand, within Pf6N2E2_3547
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0002 mg/ml remove 4,136,069 - Pf6N2E2_3545 0.21 +1.2 4,136,150 - Pf6N2E2_3545 0.30 +0.8 4,136,150 - Pf6N2E2_3545 0.30 +1.5 4,136,272 + Pf6N2E2_3545 0.43 +0.2 4,136,331 + Pf6N2E2_3545 0.49 +1.1 4,136,332 - Pf6N2E2_3545 0.49 +0.5 4,136,335 + Pf6N2E2_3545 0.49 +0.2 4,136,335 + Pf6N2E2_3545 0.49 -0.4 4,136,335 + Pf6N2E2_3545 0.49 -1.8 4,136,336 - Pf6N2E2_3545 0.49 +0.3 4,136,336 - Pf6N2E2_3545 0.49 -0.4 4,136,336 - Pf6N2E2_3545 0.49 +0.2 4,136,336 - Pf6N2E2_3545 0.49 +0.2 4,136,336 - Pf6N2E2_3545 0.49 +0.3 4,136,336 - Pf6N2E2_3545 0.49 +0.7 4,136,550 + Pf6N2E2_3545 0.72 +1.6 4,136,600 + Pf6N2E2_3545 0.77 +0.2 4,136,600 + Pf6N2E2_3545 0.77 +0.1 4,136,601 - Pf6N2E2_3545 0.77 +0.4 4,136,740 + -0.1 4,136,741 - +0.0 4,136,965 - Pf6N2E2_3546 0.15 -0.3 4,137,334 + Pf6N2E2_3546 0.52 -0.7 4,137,335 - Pf6N2E2_3546 0.52 -1.1 4,137,335 - Pf6N2E2_3546 0.52 -0.3 4,137,759 - -0.3 4,137,759 - +0.7 4,137,977 + +0.2 4,137,978 - +0.4 4,137,978 - +0.1 4,138,182 + +0.6 4,138,182 + +0.5 4,138,182 + -0.5 4,138,214 + +0.2 4,138,214 + +0.8 4,138,215 - -3.0 4,138,215 - -1.4 4,138,232 + -0.2 4,138,232 + +0.3 4,138,233 - +0.1 4,138,593 + Pf6N2E2_3547 0.31 -1.0 4,138,594 - Pf6N2E2_3547 0.31 -1.3 4,138,627 - Pf6N2E2_3547 0.33 +0.2 4,138,627 - Pf6N2E2_3547 0.33 -0.4 4,138,671 + Pf6N2E2_3547 0.37 -0.1 4,138,671 + Pf6N2E2_3547 0.37 -1.0 4,138,671 + Pf6N2E2_3547 0.37 -0.3 4,138,707 + Pf6N2E2_3547 0.40 -0.8 4,138,728 + Pf6N2E2_3547 0.41 +1.2 4,138,728 + Pf6N2E2_3547 0.41 -0.7 4,138,729 - Pf6N2E2_3547 0.42 +0.3 4,138,729 - Pf6N2E2_3547 0.42 +0.0 4,138,729 - Pf6N2E2_3547 0.42 +0.2 4,138,735 - Pf6N2E2_3547 0.42 -0.5 4,138,812 + Pf6N2E2_3547 0.48 +0.6 4,138,813 - Pf6N2E2_3547 0.48 +0.5
Or see this region's nucleotide sequence