Experiment: Tetracycline 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2418 and Pf6N2E2_2419 overlap by 17 nucleotides Pf6N2E2_2419 and Pf6N2E2_2420 are separated by 30 nucleotides Pf6N2E2_2420 and Pf6N2E2_2421 are separated by 263 nucleotides Pf6N2E2_2421 and Pf6N2E2_2422 are separated by 59 nucleotides
Pf6N2E2_2418 - DNA-binding response regulator, LuxR family, at 2,870,559 to 2,871,218
_2418
Pf6N2E2_2419 - Transcription regulator [contains diacylglycerol kinase catalytic domain], at 2,871,202 to 2,872,119
_2419
Pf6N2E2_2420 - hypothetical protein, at 2,872,150 to 2,872,317
_2420
Pf6N2E2_2421 - hypothetical protein, at 2,872,581 to 2,872,775
_2421
Pf6N2E2_2422 - Molybdopterin biosynthesis protein MoeA, at 2,872,835 to 2,874,061
_2422
Position (kb)
2872
2873 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2871.305 kb on - strand, within Pf6N2E2_2419 at 2871.305 kb on - strand, within Pf6N2E2_2419 at 2871.432 kb on + strand, within Pf6N2E2_2419 at 2871.432 kb on + strand, within Pf6N2E2_2419 at 2871.432 kb on + strand, within Pf6N2E2_2419 at 2871.432 kb on + strand, within Pf6N2E2_2419 at 2871.432 kb on + strand, within Pf6N2E2_2419 at 2871.432 kb on + strand, within Pf6N2E2_2419 at 2871.433 kb on - strand, within Pf6N2E2_2419 at 2871.433 kb on - strand, within Pf6N2E2_2419 at 2871.433 kb on - strand, within Pf6N2E2_2419 at 2871.599 kb on + strand, within Pf6N2E2_2419 at 2871.600 kb on - strand, within Pf6N2E2_2419 at 2871.644 kb on + strand, within Pf6N2E2_2419 at 2871.644 kb on + strand, within Pf6N2E2_2419 at 2871.862 kb on + strand, within Pf6N2E2_2419 at 2871.948 kb on + strand, within Pf6N2E2_2419 at 2871.948 kb on + strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2871.949 kb on - strand, within Pf6N2E2_2419 at 2872.195 kb on - strand, within Pf6N2E2_2420 at 2872.195 kb on - strand, within Pf6N2E2_2420 at 2872.281 kb on + strand, within Pf6N2E2_2420 at 2872.282 kb on - strand, within Pf6N2E2_2420 at 2872.359 kb on - strand at 2872.386 kb on + strand at 2872.387 kb on - strand at 2872.387 kb on - strand at 2872.400 kb on + strand at 2872.400 kb on + strand at 2872.401 kb on - strand at 2872.401 kb on - strand at 2872.442 kb on + strand at 2872.442 kb on + strand at 2872.442 kb on + strand at 2872.472 kb on + strand at 2872.473 kb on - strand at 2872.482 kb on + strand at 2872.482 kb on + strand at 2872.482 kb on + strand at 2872.482 kb on + strand at 2872.482 kb on + strand at 2872.483 kb on - strand at 2872.483 kb on - strand at 2872.532 kb on + strand at 2872.533 kb on - strand at 2872.559 kb on + strand at 2872.560 kb on - strand at 2872.620 kb on + strand, within Pf6N2E2_2421 at 2872.620 kb on + strand, within Pf6N2E2_2421 at 2872.620 kb on + strand, within Pf6N2E2_2421 at 2872.620 kb on + strand, within Pf6N2E2_2421 at 2872.621 kb on - strand, within Pf6N2E2_2421 at 2872.621 kb on - strand, within Pf6N2E2_2421 at 2872.621 kb on - strand, within Pf6N2E2_2421 at 2872.621 kb on - strand, within Pf6N2E2_2421 at 2872.621 kb on - strand, within Pf6N2E2_2421 at 2872.657 kb on + strand, within Pf6N2E2_2421 at 2872.657 kb on + strand, within Pf6N2E2_2421 at 2872.657 kb on + strand at 2872.658 kb on - strand, within Pf6N2E2_2421 at 2872.683 kb on + strand, within Pf6N2E2_2421 at 2872.683 kb on + strand, within Pf6N2E2_2421 at 2872.684 kb on - strand, within Pf6N2E2_2421 at 2872.684 kb on - strand, within Pf6N2E2_2421 at 2873.003 kb on + strand, within Pf6N2E2_2422 at 2873.004 kb on + strand, within Pf6N2E2_2422 at 2873.004 kb on + strand, within Pf6N2E2_2422 at 2873.004 kb on + strand, within Pf6N2E2_2422 at 2873.005 kb on - strand, within Pf6N2E2_2422 at 2873.005 kb on - strand, within Pf6N2E2_2422 at 2873.078 kb on - strand, within Pf6N2E2_2422 at 2873.093 kb on + strand, within Pf6N2E2_2422 at 2873.093 kb on + strand, within Pf6N2E2_2422 at 2873.093 kb on + strand, within Pf6N2E2_2422 at 2873.100 kb on + strand, within Pf6N2E2_2422 at 2873.159 kb on + strand, within Pf6N2E2_2422 at 2873.159 kb on + strand, within Pf6N2E2_2422 at 2873.178 kb on + strand, within Pf6N2E2_2422
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0002 mg/ml remove 2,871,305 - Pf6N2E2_2419 0.11 +0.5 2,871,305 - Pf6N2E2_2419 0.11 +0.4 2,871,432 + Pf6N2E2_2419 0.25 +0.7 2,871,432 + Pf6N2E2_2419 0.25 +0.8 2,871,432 + Pf6N2E2_2419 0.25 -0.8 2,871,432 + Pf6N2E2_2419 0.25 -0.1 2,871,432 + Pf6N2E2_2419 0.25 +0.2 2,871,432 + Pf6N2E2_2419 0.25 +0.9 2,871,433 - Pf6N2E2_2419 0.25 +0.6 2,871,433 - Pf6N2E2_2419 0.25 +2.4 2,871,433 - Pf6N2E2_2419 0.25 +0.2 2,871,599 + Pf6N2E2_2419 0.43 +0.7 2,871,600 - Pf6N2E2_2419 0.43 -0.2 2,871,644 + Pf6N2E2_2419 0.48 -0.1 2,871,644 + Pf6N2E2_2419 0.48 +0.4 2,871,862 + Pf6N2E2_2419 0.72 +0.1 2,871,948 + Pf6N2E2_2419 0.81 +0.2 2,871,948 + Pf6N2E2_2419 0.81 -0.2 2,871,949 - Pf6N2E2_2419 0.81 -1.0 2,871,949 - Pf6N2E2_2419 0.81 +1.2 2,871,949 - Pf6N2E2_2419 0.81 +0.1 2,871,949 - Pf6N2E2_2419 0.81 +0.5 2,871,949 - Pf6N2E2_2419 0.81 -0.8 2,872,195 - Pf6N2E2_2420 0.27 -1.5 2,872,195 - Pf6N2E2_2420 0.27 +0.6 2,872,281 + Pf6N2E2_2420 0.78 +1.8 2,872,282 - Pf6N2E2_2420 0.79 -0.9 2,872,359 - +0.8 2,872,386 + +0.4 2,872,387 - +0.2 2,872,387 - +0.1 2,872,400 + +0.5 2,872,400 + +0.7 2,872,401 - +1.4 2,872,401 - +0.4 2,872,442 + -0.0 2,872,442 + -0.3 2,872,442 + -0.1 2,872,472 + +0.5 2,872,473 - -0.9 2,872,482 + -0.0 2,872,482 + -0.7 2,872,482 + -0.8 2,872,482 + +0.9 2,872,482 + +0.1 2,872,483 - +0.6 2,872,483 - +0.1 2,872,532 + +0.7 2,872,533 - +0.0 2,872,559 + +1.1 2,872,560 - -0.1 2,872,620 + Pf6N2E2_2421 0.20 +0.1 2,872,620 + Pf6N2E2_2421 0.20 -0.6 2,872,620 + Pf6N2E2_2421 0.20 +0.2 2,872,620 + Pf6N2E2_2421 0.20 +0.1 2,872,621 - Pf6N2E2_2421 0.21 -0.9 2,872,621 - Pf6N2E2_2421 0.21 +0.6 2,872,621 - Pf6N2E2_2421 0.21 +0.2 2,872,621 - Pf6N2E2_2421 0.21 +0.7 2,872,621 - Pf6N2E2_2421 0.21 +0.4 2,872,657 + Pf6N2E2_2421 0.39 -0.2 2,872,657 + Pf6N2E2_2421 0.39 +0.5 2,872,657 + +0.8 2,872,658 - Pf6N2E2_2421 0.39 -0.6 2,872,683 + Pf6N2E2_2421 0.52 -0.2 2,872,683 + Pf6N2E2_2421 0.52 +0.2 2,872,684 - Pf6N2E2_2421 0.53 -0.1 2,872,684 - Pf6N2E2_2421 0.53 -0.4 2,873,003 + Pf6N2E2_2422 0.14 -2.0 2,873,004 + Pf6N2E2_2422 0.14 +0.8 2,873,004 + Pf6N2E2_2422 0.14 +0.8 2,873,004 + Pf6N2E2_2422 0.14 -1.0 2,873,005 - Pf6N2E2_2422 0.14 -0.1 2,873,005 - Pf6N2E2_2422 0.14 -0.3 2,873,078 - Pf6N2E2_2422 0.20 -0.3 2,873,093 + Pf6N2E2_2422 0.21 -0.1 2,873,093 + Pf6N2E2_2422 0.21 +0.2 2,873,093 + Pf6N2E2_2422 0.21 +0.0 2,873,100 + Pf6N2E2_2422 0.22 +0.1 2,873,159 + Pf6N2E2_2422 0.26 -1.5 2,873,159 + Pf6N2E2_2422 0.26 -0.0 2,873,178 + Pf6N2E2_2422 0.28 -0.2
Or see this region's nucleotide sequence