Experiment: Tetracycline 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2206 and Pf6N2E2_2207 are separated by 126 nucleotides Pf6N2E2_2207 and Pf6N2E2_2208 are separated by 6 nucleotides Pf6N2E2_2208 and Pf6N2E2_2209 are separated by 133 nucleotides
Pf6N2E2_2206 - VgrG protein, at 2,612,696 to 2,614,102
_2206
Pf6N2E2_2207 - cytochrome b(561), at 2,614,229 to 2,614,777
_2207
Pf6N2E2_2208 - 1-aminocyclopropane-1-carboxylate deaminase (EC 3.5.99.7), at 2,614,784 to 2,615,692
_2208
Pf6N2E2_2209 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 2,615,826 to 2,617,865
_2209
Position (kb)
2614
2615
2616 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2613.789 kb on + strand, within Pf6N2E2_2206 at 2613.789 kb on + strand, within Pf6N2E2_2206 at 2613.790 kb on - strand, within Pf6N2E2_2206 at 2613.790 kb on - strand, within Pf6N2E2_2206 at 2613.790 kb on - strand, within Pf6N2E2_2206 at 2613.849 kb on + strand, within Pf6N2E2_2206 at 2614.111 kb on + strand at 2614.111 kb on + strand at 2614.112 kb on - strand at 2614.167 kb on + strand at 2614.192 kb on - strand at 2614.192 kb on - strand at 2614.192 kb on - strand at 2614.256 kb on + strand at 2614.256 kb on + strand at 2614.261 kb on + strand at 2614.422 kb on + strand, within Pf6N2E2_2207 at 2614.423 kb on - strand, within Pf6N2E2_2207 at 2614.521 kb on - strand, within Pf6N2E2_2207 at 2614.521 kb on - strand, within Pf6N2E2_2207 at 2614.521 kb on - strand, within Pf6N2E2_2207 at 2614.521 kb on - strand, within Pf6N2E2_2207 at 2614.521 kb on - strand, within Pf6N2E2_2207 at 2614.592 kb on + strand, within Pf6N2E2_2207 at 2614.593 kb on - strand, within Pf6N2E2_2207 at 2614.727 kb on + strand at 2614.727 kb on + strand at 2614.728 kb on - strand at 2614.728 kb on - strand at 2614.728 kb on - strand at 2614.902 kb on + strand, within Pf6N2E2_2208 at 2614.903 kb on - strand, within Pf6N2E2_2208 at 2615.001 kb on + strand, within Pf6N2E2_2208 at 2615.001 kb on + strand, within Pf6N2E2_2208 at 2615.001 kb on + strand, within Pf6N2E2_2208 at 2615.340 kb on + strand, within Pf6N2E2_2208 at 2615.481 kb on + strand, within Pf6N2E2_2208 at 2615.481 kb on + strand, within Pf6N2E2_2208 at 2615.726 kb on + strand at 2615.727 kb on - strand at 2615.811 kb on + strand at 2615.811 kb on + strand at 2615.811 kb on + strand at 2615.812 kb on - strand at 2615.812 kb on - strand at 2615.827 kb on + strand at 2615.827 kb on + strand at 2615.828 kb on - strand at 2615.828 kb on - strand at 2616.108 kb on - strand, within Pf6N2E2_2209 at 2616.400 kb on + strand, within Pf6N2E2_2209 at 2616.401 kb on - strand, within Pf6N2E2_2209 at 2616.401 kb on - strand, within Pf6N2E2_2209 at 2616.566 kb on - strand, within Pf6N2E2_2209
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0002 mg/ml remove 2,613,789 + Pf6N2E2_2206 0.78 -0.6 2,613,789 + Pf6N2E2_2206 0.78 -0.1 2,613,790 - Pf6N2E2_2206 0.78 +0.6 2,613,790 - Pf6N2E2_2206 0.78 +0.5 2,613,790 - Pf6N2E2_2206 0.78 -0.5 2,613,849 + Pf6N2E2_2206 0.82 +0.7 2,614,111 + +0.9 2,614,111 + +0.5 2,614,112 - -0.4 2,614,167 + +0.6 2,614,192 - +0.5 2,614,192 - -0.1 2,614,192 - +0.0 2,614,256 + -0.3 2,614,256 + -0.6 2,614,261 + +1.4 2,614,422 + Pf6N2E2_2207 0.35 +0.7 2,614,423 - Pf6N2E2_2207 0.35 +0.7 2,614,521 - Pf6N2E2_2207 0.53 +0.0 2,614,521 - Pf6N2E2_2207 0.53 -3.2 2,614,521 - Pf6N2E2_2207 0.53 +1.6 2,614,521 - Pf6N2E2_2207 0.53 -0.6 2,614,521 - Pf6N2E2_2207 0.53 +0.6 2,614,592 + Pf6N2E2_2207 0.66 +0.0 2,614,593 - Pf6N2E2_2207 0.66 -0.4 2,614,727 + -0.4 2,614,727 + -0.3 2,614,728 - -0.9 2,614,728 - -0.3 2,614,728 - -0.6 2,614,902 + Pf6N2E2_2208 0.13 +0.9 2,614,903 - Pf6N2E2_2208 0.13 -1.1 2,615,001 + Pf6N2E2_2208 0.24 -0.5 2,615,001 + Pf6N2E2_2208 0.24 -0.5 2,615,001 + Pf6N2E2_2208 0.24 -3.4 2,615,340 + Pf6N2E2_2208 0.61 -0.4 2,615,481 + Pf6N2E2_2208 0.77 -1.3 2,615,481 + Pf6N2E2_2208 0.77 +0.2 2,615,726 + +0.1 2,615,727 - +0.6 2,615,811 + -1.2 2,615,811 + +0.1 2,615,811 + -0.4 2,615,812 - -0.1 2,615,812 - +0.0 2,615,827 + +1.3 2,615,827 + -0.1 2,615,828 - -0.1 2,615,828 - +0.9 2,616,108 - Pf6N2E2_2209 0.14 -1.2 2,616,400 + Pf6N2E2_2209 0.28 +0.3 2,616,401 - Pf6N2E2_2209 0.28 -0.1 2,616,401 - Pf6N2E2_2209 0.28 -0.6 2,616,566 - Pf6N2E2_2209 0.36 -0.2
Or see this region's nucleotide sequence