Experiment: Tetracycline 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_215 and Pf6N2E2_216 are separated by 225 nucleotides Pf6N2E2_216 and Pf6N2E2_217 overlap by 4 nucleotides Pf6N2E2_217 and Pf6N2E2_218 are separated by 107 nucleotides Pf6N2E2_218 and Pf6N2E2_219 are separated by 21 nucleotides
Pf6N2E2_215 - Outer membrane protein assembly factor YaeT precursor, at 271,909 to 274,275
_215
Pf6N2E2_216 - Copper-sensing two-component system response regulator CpxR, at 274,501 to 275,184
_216
Pf6N2E2_217 - Signal transduction histidine kinase, at 275,181 to 276,443
_217
Pf6N2E2_218 - hypothetical protein, at 276,551 to 277,000
_218
Pf6N2E2_219 - Response regulator BaeR, at 277,022 to 277,705
_219
Position (kb) 275
276
277 Strain fitness (log2 ratio) -2
-1
0
1
2 at 274.310 kb on + strand at 274.310 kb on + strand at 274.310 kb on + strand at 274.310 kb on + strand at 274.310 kb on + strand at 274.311 kb on - strand at 274.311 kb on - strand at 274.311 kb on - strand at 274.311 kb on - strand at 274.380 kb on + strand at 274.422 kb on + strand at 274.423 kb on - strand at 274.426 kb on + strand at 274.485 kb on + strand at 274.485 kb on + strand at 274.511 kb on + strand at 274.511 kb on + strand at 274.512 kb on - strand at 274.512 kb on - strand at 274.638 kb on + strand, within Pf6N2E2_216 at 274.638 kb on + strand, within Pf6N2E2_216 at 274.638 kb on + strand, within Pf6N2E2_216 at 274.639 kb on - strand, within Pf6N2E2_216 at 274.640 kb on - strand, within Pf6N2E2_216 at 274.792 kb on + strand, within Pf6N2E2_216 at 274.792 kb on + strand, within Pf6N2E2_216 at 274.792 kb on + strand, within Pf6N2E2_216 at 274.793 kb on - strand, within Pf6N2E2_216 at 275.066 kb on - strand, within Pf6N2E2_216 at 275.164 kb on + strand at 275.322 kb on - strand, within Pf6N2E2_217 at 275.335 kb on + strand, within Pf6N2E2_217 at 275.335 kb on + strand, within Pf6N2E2_217 at 275.336 kb on - strand, within Pf6N2E2_217 at 275.336 kb on - strand, within Pf6N2E2_217 at 275.415 kb on + strand, within Pf6N2E2_217 at 275.467 kb on - strand, within Pf6N2E2_217 at 275.607 kb on + strand, within Pf6N2E2_217 at 275.607 kb on + strand, within Pf6N2E2_217 at 275.620 kb on - strand, within Pf6N2E2_217 at 275.779 kb on + strand, within Pf6N2E2_217 at 276.456 kb on - strand at 276.458 kb on + strand at 276.458 kb on + strand at 276.458 kb on + strand at 276.458 kb on + strand at 276.458 kb on + strand at 276.479 kb on + strand at 276.565 kb on + strand at 276.565 kb on + strand at 276.565 kb on + strand at 276.566 kb on - strand at 276.614 kb on + strand, within Pf6N2E2_218 at 276.614 kb on + strand, within Pf6N2E2_218 at 276.614 kb on + strand, within Pf6N2E2_218 at 276.798 kb on + strand, within Pf6N2E2_218 at 276.798 kb on + strand, within Pf6N2E2_218 at 276.799 kb on - strand, within Pf6N2E2_218 at 276.935 kb on + strand, within Pf6N2E2_218 at 276.935 kb on + strand, within Pf6N2E2_218 at 276.979 kb on + strand at 276.983 kb on + strand at 276.983 kb on + strand at 276.983 kb on + strand at 276.984 kb on - strand at 276.998 kb on + strand at 276.999 kb on - strand at 277.060 kb on - strand at 277.060 kb on - strand at 277.060 kb on - strand at 277.060 kb on - strand at 277.060 kb on - strand at 277.167 kb on + strand, within Pf6N2E2_219 at 277.167 kb on + strand, within Pf6N2E2_219 at 277.167 kb on + strand, within Pf6N2E2_219 at 277.168 kb on - strand, within Pf6N2E2_219 at 277.225 kb on + strand, within Pf6N2E2_219 at 277.225 kb on + strand, within Pf6N2E2_219 at 277.226 kb on - strand, within Pf6N2E2_219 at 277.404 kb on + strand, within Pf6N2E2_219 at 277.404 kb on + strand, within Pf6N2E2_219 at 277.405 kb on - strand, within Pf6N2E2_219 at 277.405 kb on - strand, within Pf6N2E2_219
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0002 mg/ml remove 274,310 + -1.3 274,310 + -0.0 274,310 + +0.2 274,310 + -0.8 274,310 + +0.0 274,311 - +0.4 274,311 - +0.7 274,311 - +0.8 274,311 - +0.5 274,380 + -1.9 274,422 + +0.1 274,423 - +1.0 274,426 + +0.9 274,485 + +0.0 274,485 + +0.4 274,511 + +0.3 274,511 + -0.5 274,512 - +0.1 274,512 - -0.6 274,638 + Pf6N2E2_216 0.20 +0.7 274,638 + Pf6N2E2_216 0.20 +0.4 274,638 + Pf6N2E2_216 0.20 +0.1 274,639 - Pf6N2E2_216 0.20 +0.0 274,640 - Pf6N2E2_216 0.20 -1.5 274,792 + Pf6N2E2_216 0.43 +0.7 274,792 + Pf6N2E2_216 0.43 +0.9 274,792 + Pf6N2E2_216 0.43 +0.2 274,793 - Pf6N2E2_216 0.43 -0.6 275,066 - Pf6N2E2_216 0.83 +0.1 275,164 + -0.4 275,322 - Pf6N2E2_217 0.11 -0.8 275,335 + Pf6N2E2_217 0.12 -1.1 275,335 + Pf6N2E2_217 0.12 -1.1 275,336 - Pf6N2E2_217 0.12 +1.0 275,336 - Pf6N2E2_217 0.12 -1.9 275,415 + Pf6N2E2_217 0.19 -1.1 275,467 - Pf6N2E2_217 0.23 -2.5 275,607 + Pf6N2E2_217 0.34 -0.1 275,607 + Pf6N2E2_217 0.34 +0.3 275,620 - Pf6N2E2_217 0.35 +0.2 275,779 + Pf6N2E2_217 0.47 +0.2 276,456 - +1.0 276,458 + -0.8 276,458 + +0.1 276,458 + -0.5 276,458 + -0.9 276,458 + +0.1 276,479 + -1.3 276,565 + +0.9 276,565 + +0.2 276,565 + +0.4 276,566 - -0.3 276,614 + Pf6N2E2_218 0.14 -1.0 276,614 + Pf6N2E2_218 0.14 +0.6 276,614 + Pf6N2E2_218 0.14 -0.7 276,798 + Pf6N2E2_218 0.55 +0.3 276,798 + Pf6N2E2_218 0.55 +0.3 276,799 - Pf6N2E2_218 0.55 +1.9 276,935 + Pf6N2E2_218 0.85 +0.6 276,935 + Pf6N2E2_218 0.85 -0.7 276,979 + -0.1 276,983 + +0.1 276,983 + +0.4 276,983 + +0.1 276,984 - +1.5 276,998 + +0.2 276,999 - +1.5 277,060 - -0.1 277,060 - +0.2 277,060 - -0.9 277,060 - +0.4 277,060 - +0.3 277,167 + Pf6N2E2_219 0.21 +0.9 277,167 + Pf6N2E2_219 0.21 +0.2 277,167 + Pf6N2E2_219 0.21 -0.5 277,168 - Pf6N2E2_219 0.21 +0.2 277,225 + Pf6N2E2_219 0.30 +0.3 277,225 + Pf6N2E2_219 0.30 +1.4 277,226 - Pf6N2E2_219 0.30 +0.7 277,404 + Pf6N2E2_219 0.56 -0.8 277,404 + Pf6N2E2_219 0.56 -1.3 277,405 - Pf6N2E2_219 0.56 +0.9 277,405 - Pf6N2E2_219 0.56 +0.3
Or see this region's nucleotide sequence