Experiment: Tetracycline 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1232 and Pf6N2E2_1233 are separated by 14 nucleotides Pf6N2E2_1233 and Pf6N2E2_1234 are separated by 139 nucleotides Pf6N2E2_1234 and Pf6N2E2_1235 are separated by 140 nucleotides
Pf6N2E2_1232 - ABC-type protease exporter, membrane fusion protein (MFP) family component PrtE/AprE, at 1,443,442 to 1,444,758
_1232
Pf6N2E2_1233 - ABC-type protease exporter, ATP-binding component PrtD/AprD, at 1,444,773 to 1,446,572
_1233
Pf6N2E2_1234 - hypothetical protein, at 1,446,712 to 1,447,065
_1234
Pf6N2E2_1235 - Secreted alkaline metalloproteinase (EC 3.4.24.-), PrtA/B/C/G homolog, at 1,447,206 to 1,448,663
_1235
Position (kb)
1444
1445
1446
1447 Strain fitness (log2 ratio)
-2
-1
0
1 at 1443.992 kb on + strand, within Pf6N2E2_1232 at 1444.136 kb on + strand, within Pf6N2E2_1232 at 1444.136 kb on + strand, within Pf6N2E2_1232 at 1444.136 kb on + strand, within Pf6N2E2_1232 at 1444.137 kb on - strand, within Pf6N2E2_1232 at 1444.137 kb on - strand, within Pf6N2E2_1232 at 1444.137 kb on - strand, within Pf6N2E2_1232 at 1444.381 kb on + strand, within Pf6N2E2_1232 at 1444.381 kb on + strand, within Pf6N2E2_1232 at 1444.381 kb on + strand, within Pf6N2E2_1232 at 1444.382 kb on - strand, within Pf6N2E2_1232 at 1444.382 kb on - strand, within Pf6N2E2_1232 at 1444.743 kb on + strand at 1444.743 kb on + strand at 1444.743 kb on + strand at 1444.743 kb on + strand at 1444.743 kb on + strand at 1444.744 kb on - strand at 1444.938 kb on + strand at 1445.122 kb on + strand, within Pf6N2E2_1233 at 1445.122 kb on + strand, within Pf6N2E2_1233 at 1445.123 kb on - strand, within Pf6N2E2_1233 at 1445.123 kb on - strand, within Pf6N2E2_1233 at 1445.123 kb on - strand, within Pf6N2E2_1233 at 1445.200 kb on + strand, within Pf6N2E2_1233 at 1445.200 kb on + strand, within Pf6N2E2_1233 at 1445.201 kb on - strand, within Pf6N2E2_1233 at 1445.781 kb on + strand, within Pf6N2E2_1233 at 1445.782 kb on - strand, within Pf6N2E2_1233 at 1446.119 kb on - strand, within Pf6N2E2_1233 at 1446.134 kb on - strand, within Pf6N2E2_1233 at 1446.134 kb on - strand, within Pf6N2E2_1233 at 1446.445 kb on + strand at 1446.703 kb on - strand at 1446.703 kb on - strand at 1446.703 kb on - strand at 1446.779 kb on + strand, within Pf6N2E2_1234 at 1446.782 kb on + strand, within Pf6N2E2_1234 at 1446.782 kb on + strand, within Pf6N2E2_1234 at 1447.383 kb on - strand, within Pf6N2E2_1235
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0002 mg/ml remove 1,443,992 + Pf6N2E2_1232 0.42 +0.8 1,444,136 + Pf6N2E2_1232 0.53 -0.8 1,444,136 + Pf6N2E2_1232 0.53 +0.4 1,444,136 + Pf6N2E2_1232 0.53 -0.2 1,444,137 - Pf6N2E2_1232 0.53 +0.7 1,444,137 - Pf6N2E2_1232 0.53 -0.2 1,444,137 - Pf6N2E2_1232 0.53 -0.1 1,444,381 + Pf6N2E2_1232 0.71 -0.5 1,444,381 + Pf6N2E2_1232 0.71 +1.2 1,444,381 + Pf6N2E2_1232 0.71 -0.9 1,444,382 - Pf6N2E2_1232 0.71 -0.4 1,444,382 - Pf6N2E2_1232 0.71 -0.2 1,444,743 + +1.1 1,444,743 + -0.7 1,444,743 + +0.4 1,444,743 + -0.3 1,444,743 + +0.2 1,444,744 - +0.1 1,444,938 + -1.1 1,445,122 + Pf6N2E2_1233 0.19 +0.0 1,445,122 + Pf6N2E2_1233 0.19 -0.4 1,445,123 - Pf6N2E2_1233 0.19 -0.4 1,445,123 - Pf6N2E2_1233 0.19 +0.4 1,445,123 - Pf6N2E2_1233 0.19 -0.7 1,445,200 + Pf6N2E2_1233 0.24 -0.6 1,445,200 + Pf6N2E2_1233 0.24 -0.7 1,445,201 - Pf6N2E2_1233 0.24 -0.9 1,445,781 + Pf6N2E2_1233 0.56 -2.6 1,445,782 - Pf6N2E2_1233 0.56 +0.4 1,446,119 - Pf6N2E2_1233 0.75 -0.7 1,446,134 - Pf6N2E2_1233 0.76 +0.4 1,446,134 - Pf6N2E2_1233 0.76 -1.2 1,446,445 + -0.3 1,446,703 - +1.3 1,446,703 - -0.2 1,446,703 - -0.4 1,446,779 + Pf6N2E2_1234 0.19 -0.6 1,446,782 + Pf6N2E2_1234 0.20 +1.2 1,446,782 + Pf6N2E2_1234 0.20 +0.6 1,447,383 - Pf6N2E2_1235 0.12 +0.2
Or see this region's nucleotide sequence