Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1233

Experiment: Tetracycline 0.0002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1232 and Pf6N2E2_1233 are separated by 14 nucleotidesPf6N2E2_1233 and Pf6N2E2_1234 are separated by 139 nucleotidesPf6N2E2_1234 and Pf6N2E2_1235 are separated by 140 nucleotides Pf6N2E2_1232 - ABC-type protease exporter, membrane fusion protein (MFP) family component PrtE/AprE, at 1,443,442 to 1,444,758 _1232 Pf6N2E2_1233 - ABC-type protease exporter, ATP-binding component PrtD/AprD, at 1,444,773 to 1,446,572 _1233 Pf6N2E2_1234 - hypothetical protein, at 1,446,712 to 1,447,065 _1234 Pf6N2E2_1235 - Secreted alkaline metalloproteinase (EC 3.4.24.-), PrtA/B/C/G homolog, at 1,447,206 to 1,448,663 _1235 Position (kb) 1444 1445 1446 1447Strain fitness (log2 ratio) -2 -1 0 1at 1443.992 kb on + strand, within Pf6N2E2_1232at 1444.136 kb on + strand, within Pf6N2E2_1232at 1444.136 kb on + strand, within Pf6N2E2_1232at 1444.136 kb on + strand, within Pf6N2E2_1232at 1444.137 kb on - strand, within Pf6N2E2_1232at 1444.137 kb on - strand, within Pf6N2E2_1232at 1444.137 kb on - strand, within Pf6N2E2_1232at 1444.381 kb on + strand, within Pf6N2E2_1232at 1444.381 kb on + strand, within Pf6N2E2_1232at 1444.381 kb on + strand, within Pf6N2E2_1232at 1444.382 kb on - strand, within Pf6N2E2_1232at 1444.382 kb on - strand, within Pf6N2E2_1232at 1444.743 kb on + strandat 1444.743 kb on + strandat 1444.743 kb on + strandat 1444.743 kb on + strandat 1444.743 kb on + strandat 1444.744 kb on - strandat 1444.938 kb on + strandat 1445.122 kb on + strand, within Pf6N2E2_1233at 1445.122 kb on + strand, within Pf6N2E2_1233at 1445.123 kb on - strand, within Pf6N2E2_1233at 1445.123 kb on - strand, within Pf6N2E2_1233at 1445.123 kb on - strand, within Pf6N2E2_1233at 1445.200 kb on + strand, within Pf6N2E2_1233at 1445.200 kb on + strand, within Pf6N2E2_1233at 1445.201 kb on - strand, within Pf6N2E2_1233at 1445.781 kb on + strand, within Pf6N2E2_1233at 1445.782 kb on - strand, within Pf6N2E2_1233at 1446.119 kb on - strand, within Pf6N2E2_1233at 1446.134 kb on - strand, within Pf6N2E2_1233at 1446.134 kb on - strand, within Pf6N2E2_1233at 1446.445 kb on + strandat 1446.703 kb on - strandat 1446.703 kb on - strandat 1446.703 kb on - strandat 1446.779 kb on + strand, within Pf6N2E2_1234at 1446.782 kb on + strand, within Pf6N2E2_1234at 1446.782 kb on + strand, within Pf6N2E2_1234at 1447.383 kb on - strand, within Pf6N2E2_1235

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Per-strain Table

Position Strand Gene LocusTag Fraction Tetracycline 0.0002 mg/ml
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1,443,992 + Pf6N2E2_1232 0.42 +0.8
1,444,136 + Pf6N2E2_1232 0.53 -0.8
1,444,136 + Pf6N2E2_1232 0.53 +0.4
1,444,136 + Pf6N2E2_1232 0.53 -0.2
1,444,137 - Pf6N2E2_1232 0.53 +0.7
1,444,137 - Pf6N2E2_1232 0.53 -0.2
1,444,137 - Pf6N2E2_1232 0.53 -0.1
1,444,381 + Pf6N2E2_1232 0.71 -0.5
1,444,381 + Pf6N2E2_1232 0.71 +1.2
1,444,381 + Pf6N2E2_1232 0.71 -0.9
1,444,382 - Pf6N2E2_1232 0.71 -0.4
1,444,382 - Pf6N2E2_1232 0.71 -0.2
1,444,743 + +1.1
1,444,743 + -0.7
1,444,743 + +0.4
1,444,743 + -0.3
1,444,743 + +0.2
1,444,744 - +0.1
1,444,938 + -1.1
1,445,122 + Pf6N2E2_1233 0.19 +0.0
1,445,122 + Pf6N2E2_1233 0.19 -0.4
1,445,123 - Pf6N2E2_1233 0.19 -0.4
1,445,123 - Pf6N2E2_1233 0.19 +0.4
1,445,123 - Pf6N2E2_1233 0.19 -0.7
1,445,200 + Pf6N2E2_1233 0.24 -0.6
1,445,200 + Pf6N2E2_1233 0.24 -0.7
1,445,201 - Pf6N2E2_1233 0.24 -0.9
1,445,781 + Pf6N2E2_1233 0.56 -2.6
1,445,782 - Pf6N2E2_1233 0.56 +0.4
1,446,119 - Pf6N2E2_1233 0.75 -0.7
1,446,134 - Pf6N2E2_1233 0.76 +0.4
1,446,134 - Pf6N2E2_1233 0.76 -1.2
1,446,445 + -0.3
1,446,703 - +1.3
1,446,703 - -0.2
1,446,703 - -0.4
1,446,779 + Pf6N2E2_1234 0.19 -0.6
1,446,782 + Pf6N2E2_1234 0.20 +1.2
1,446,782 + Pf6N2E2_1234 0.20 +0.6
1,447,383 - Pf6N2E2_1235 0.12 +0.2

Or see this region's nucleotide sequence