Experiment: Tetracycline 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1003 and Pf6N2E2_1004 are separated by 88 nucleotides Pf6N2E2_1004 and Pf6N2E2_1005 are separated by 85 nucleotides Pf6N2E2_1005 and Pf6N2E2_1006 are separated by 486 nucleotides
Pf6N2E2_1003 - Fucose permease, at 1,172,313 to 1,173,695
_1003
Pf6N2E2_1004 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 1,173,784 to 1,174,542
_1004
Pf6N2E2_1005 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 1,174,628 to 1,175,581
_1005
Pf6N2E2_1006 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 1,176,068 to 1,176,601
_1006
Position (kb)
1174
1175
1176 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1173.684 kb on - strand at 1173.722 kb on + strand at 1173.722 kb on + strand at 1173.778 kb on + strand at 1173.779 kb on - strand at 1173.816 kb on + strand at 1173.827 kb on + strand at 1173.827 kb on + strand at 1173.827 kb on + strand at 1173.828 kb on - strand at 1173.831 kb on + strand at 1173.831 kb on + strand at 1173.832 kb on - strand at 1173.832 kb on - strand at 1173.832 kb on - strand at 1173.832 kb on - strand at 1173.832 kb on - strand at 1173.946 kb on + strand, within Pf6N2E2_1004 at 1173.947 kb on - strand, within Pf6N2E2_1004 at 1173.947 kb on - strand, within Pf6N2E2_1004 at 1173.947 kb on - strand, within Pf6N2E2_1004 at 1173.958 kb on - strand, within Pf6N2E2_1004 at 1174.023 kb on - strand, within Pf6N2E2_1004 at 1174.049 kb on + strand, within Pf6N2E2_1004 at 1174.049 kb on + strand, within Pf6N2E2_1004 at 1174.074 kb on - strand, within Pf6N2E2_1004 at 1174.081 kb on + strand, within Pf6N2E2_1004 at 1174.132 kb on - strand, within Pf6N2E2_1004 at 1174.153 kb on + strand, within Pf6N2E2_1004 at 1174.313 kb on - strand, within Pf6N2E2_1004 at 1174.422 kb on + strand, within Pf6N2E2_1004 at 1174.423 kb on - strand, within Pf6N2E2_1004 at 1174.423 kb on - strand, within Pf6N2E2_1004 at 1174.423 kb on - strand, within Pf6N2E2_1004 at 1174.466 kb on + strand, within Pf6N2E2_1004 at 1174.466 kb on + strand, within Pf6N2E2_1004 at 1174.693 kb on - strand at 1174.759 kb on + strand, within Pf6N2E2_1005 at 1174.759 kb on + strand, within Pf6N2E2_1005 at 1174.763 kb on + strand, within Pf6N2E2_1005 at 1174.763 kb on + strand, within Pf6N2E2_1005 at 1174.763 kb on + strand, within Pf6N2E2_1005 at 1174.763 kb on + strand, within Pf6N2E2_1005 at 1174.764 kb on - strand, within Pf6N2E2_1005 at 1174.764 kb on - strand, within Pf6N2E2_1005 at 1174.764 kb on - strand, within Pf6N2E2_1005 at 1174.806 kb on + strand, within Pf6N2E2_1005 at 1174.806 kb on + strand, within Pf6N2E2_1005 at 1174.806 kb on + strand, within Pf6N2E2_1005 at 1174.807 kb on - strand, within Pf6N2E2_1005 at 1174.947 kb on + strand, within Pf6N2E2_1005 at 1174.947 kb on + strand, within Pf6N2E2_1005 at 1174.947 kb on + strand, within Pf6N2E2_1005 at 1174.948 kb on - strand, within Pf6N2E2_1005 at 1175.013 kb on - strand, within Pf6N2E2_1005 at 1175.048 kb on - strand, within Pf6N2E2_1005 at 1175.048 kb on - strand, within Pf6N2E2_1005 at 1175.285 kb on - strand, within Pf6N2E2_1005 at 1175.564 kb on + strand at 1175.565 kb on - strand at 1175.565 kb on - strand at 1175.593 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.621 kb on + strand at 1175.622 kb on - strand at 1175.625 kb on + strand at 1175.626 kb on - strand at 1175.676 kb on + strand at 1175.813 kb on - strand at 1175.916 kb on + strand at 1175.964 kb on + strand at 1175.964 kb on + strand at 1175.964 kb on + strand at 1175.965 kb on - strand at 1175.965 kb on - strand at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.538 kb on + strand, within Pf6N2E2_1006 at 1176.539 kb on - strand, within Pf6N2E2_1006
Per-strain Table
Position Strand Gene LocusTag Fraction Tetracycline 0.0002 mg/ml remove 1,173,684 - +0.6 1,173,722 + +0.3 1,173,722 + +1.3 1,173,778 + +1.1 1,173,779 - +0.8 1,173,816 + +1.0 1,173,827 + +2.1 1,173,827 + +0.3 1,173,827 + -0.5 1,173,828 - +0.8 1,173,831 + -0.5 1,173,831 + -1.2 1,173,832 - +1.5 1,173,832 - +0.0 1,173,832 - +0.3 1,173,832 - -0.9 1,173,832 - +1.5 1,173,946 + Pf6N2E2_1004 0.21 -0.7 1,173,947 - Pf6N2E2_1004 0.21 +0.7 1,173,947 - Pf6N2E2_1004 0.21 -0.5 1,173,947 - Pf6N2E2_1004 0.21 -0.4 1,173,958 - Pf6N2E2_1004 0.23 +0.4 1,174,023 - Pf6N2E2_1004 0.31 -0.3 1,174,049 + Pf6N2E2_1004 0.35 +1.6 1,174,049 + Pf6N2E2_1004 0.35 +2.0 1,174,074 - Pf6N2E2_1004 0.38 -0.7 1,174,081 + Pf6N2E2_1004 0.39 -0.3 1,174,132 - Pf6N2E2_1004 0.46 -0.7 1,174,153 + Pf6N2E2_1004 0.49 -2.3 1,174,313 - Pf6N2E2_1004 0.70 +0.2 1,174,422 + Pf6N2E2_1004 0.84 +0.7 1,174,423 - Pf6N2E2_1004 0.84 +0.0 1,174,423 - Pf6N2E2_1004 0.84 +1.0 1,174,423 - Pf6N2E2_1004 0.84 +0.1 1,174,466 + Pf6N2E2_1004 0.90 -0.1 1,174,466 + Pf6N2E2_1004 0.90 -0.3 1,174,693 - +0.5 1,174,759 + Pf6N2E2_1005 0.14 +1.1 1,174,759 + Pf6N2E2_1005 0.14 -0.5 1,174,763 + Pf6N2E2_1005 0.14 -0.5 1,174,763 + Pf6N2E2_1005 0.14 -0.2 1,174,763 + Pf6N2E2_1005 0.14 -0.1 1,174,763 + Pf6N2E2_1005 0.14 +0.2 1,174,764 - Pf6N2E2_1005 0.14 -0.6 1,174,764 - Pf6N2E2_1005 0.14 -0.4 1,174,764 - Pf6N2E2_1005 0.14 +1.1 1,174,806 + Pf6N2E2_1005 0.19 -0.8 1,174,806 + Pf6N2E2_1005 0.19 -1.1 1,174,806 + Pf6N2E2_1005 0.19 +0.8 1,174,807 - Pf6N2E2_1005 0.19 -1.7 1,174,947 + Pf6N2E2_1005 0.33 -0.3 1,174,947 + Pf6N2E2_1005 0.33 -0.9 1,174,947 + Pf6N2E2_1005 0.33 +1.1 1,174,948 - Pf6N2E2_1005 0.34 -0.6 1,175,013 - Pf6N2E2_1005 0.40 -1.5 1,175,048 - Pf6N2E2_1005 0.44 -1.5 1,175,048 - Pf6N2E2_1005 0.44 -0.5 1,175,285 - Pf6N2E2_1005 0.69 +0.5 1,175,564 + -1.5 1,175,565 - -0.3 1,175,565 - +0.4 1,175,593 + +0.6 1,175,621 + -1.3 1,175,621 + -0.3 1,175,621 + -0.5 1,175,621 + +0.5 1,175,621 + -0.7 1,175,621 + +0.8 1,175,621 + -0.1 1,175,621 + +0.0 1,175,622 - -1.3 1,175,625 + +0.6 1,175,626 - +0.1 1,175,676 + +1.5 1,175,813 - -0.0 1,175,916 + -0.7 1,175,964 + -0.1 1,175,964 + -0.7 1,175,964 + -0.4 1,175,965 - -1.7 1,175,965 - +0.6 1,176,538 + Pf6N2E2_1006 0.88 +0.7 1,176,538 + Pf6N2E2_1006 0.88 +0.3 1,176,538 + Pf6N2E2_1006 0.88 -1.5 1,176,538 + Pf6N2E2_1006 0.88 +1.0 1,176,538 + Pf6N2E2_1006 0.88 -1.0 1,176,539 - Pf6N2E2_1006 0.88 +0.9
Or see this region's nucleotide sequence