Experiment: Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20190 and IAI47_20195 are separated by 98 nucleotides IAI47_20195 and IAI47_20200 are separated by 99 nucleotides IAI47_20200 and IAI47_20205 overlap by 16 nucleotides
IAI47_20190: IAI47_20190 - urea carboxylase, at 269,444 to 273,049
_20190
IAI47_20195: IAI47_20195 - LysR family transcriptional regulator, at 273,148 to 274,053
_20195
IAI47_20200: IAI47_20200 - 2OG-Fe(II) oxygenase, at 274,153 to 274,926
_20200
IAI47_20205: IAI47_20205 - DUF2817 domain-containing protein, at 274,911 to 275,996
_20205
Position (kb) 273
274
275 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 272.159 kb on + strand, within IAI47_20190 at 272.160 kb on - strand, within IAI47_20190 at 272.160 kb on - strand, within IAI47_20190 at 272.161 kb on + strand, within IAI47_20190 at 272.162 kb on - strand, within IAI47_20190 at 272.162 kb on - strand, within IAI47_20190 at 272.162 kb on - strand, within IAI47_20190 at 272.167 kb on + strand, within IAI47_20190 at 272.167 kb on + strand, within IAI47_20190 at 272.168 kb on - strand, within IAI47_20190 at 272.168 kb on - strand, within IAI47_20190 at 272.201 kb on - strand, within IAI47_20190 at 272.201 kb on - strand, within IAI47_20190 at 272.276 kb on + strand, within IAI47_20190 at 272.276 kb on + strand, within IAI47_20190 at 272.277 kb on - strand, within IAI47_20190 at 272.303 kb on + strand, within IAI47_20190 at 272.304 kb on - strand, within IAI47_20190 at 272.304 kb on - strand, within IAI47_20190 at 272.304 kb on - strand, within IAI47_20190 at 272.304 kb on - strand, within IAI47_20190 at 272.363 kb on + strand, within IAI47_20190 at 272.363 kb on + strand, within IAI47_20190 at 272.363 kb on + strand, within IAI47_20190 at 272.364 kb on - strand, within IAI47_20190 at 272.364 kb on - strand, within IAI47_20190 at 272.364 kb on - strand, within IAI47_20190 at 272.364 kb on - strand, within IAI47_20190 at 272.365 kb on + strand, within IAI47_20190 at 272.365 kb on + strand, within IAI47_20190 at 272.417 kb on + strand, within IAI47_20190 at 272.429 kb on + strand, within IAI47_20190 at 272.529 kb on + strand, within IAI47_20190 at 272.529 kb on + strand, within IAI47_20190 at 272.529 kb on + strand, within IAI47_20190 at 272.530 kb on - strand, within IAI47_20190 at 272.627 kb on - strand, within IAI47_20190 at 272.657 kb on + strand, within IAI47_20190 at 272.658 kb on - strand, within IAI47_20190 at 272.658 kb on - strand, within IAI47_20190 at 272.690 kb on - strand at 272.690 kb on - strand at 272.859 kb on + strand at 272.868 kb on + strand at 272.868 kb on + strand at 272.869 kb on - strand at 272.869 kb on - strand at 272.965 kb on + strand at 272.965 kb on + strand at 272.965 kb on + strand at 272.966 kb on - strand at 272.966 kb on - strand at 272.985 kb on + strand at 272.985 kb on + strand at 272.985 kb on + strand at 272.986 kb on - strand at 273.047 kb on + strand at 273.047 kb on + strand at 273.048 kb on - strand at 273.057 kb on + strand at 273.057 kb on + strand at 273.057 kb on + strand at 273.057 kb on + strand at 273.057 kb on + strand at 273.057 kb on + strand at 273.059 kb on + strand at 273.059 kb on + strand at 273.059 kb on + strand at 273.059 kb on + strand at 273.059 kb on + strand at 273.059 kb on + strand at 273.059 kb on + strand at 273.060 kb on - strand at 273.060 kb on - strand at 273.060 kb on - strand at 273.060 kb on - strand at 273.064 kb on + strand at 273.065 kb on - strand at 273.065 kb on - strand at 273.072 kb on + strand at 273.072 kb on + strand at 273.072 kb on + strand at 273.072 kb on + strand at 273.073 kb on - strand at 273.073 kb on - strand at 273.073 kb on - strand at 273.110 kb on + strand at 273.110 kb on + strand at 273.111 kb on - strand at 273.111 kb on - strand at 273.133 kb on + strand at 273.133 kb on + strand at 273.133 kb on + strand at 273.134 kb on - strand at 273.236 kb on + strand at 273.237 kb on - strand at 273.237 kb on - strand at 273.272 kb on - strand, within IAI47_20195 at 273.376 kb on - strand, within IAI47_20195 at 273.381 kb on + strand, within IAI47_20195 at 273.381 kb on + strand, within IAI47_20195 at 273.382 kb on - strand, within IAI47_20195 at 273.664 kb on + strand, within IAI47_20195 at 273.664 kb on + strand, within IAI47_20195 at 273.664 kb on + strand, within IAI47_20195 at 273.665 kb on - strand, within IAI47_20195 at 273.665 kb on - strand, within IAI47_20195 at 273.665 kb on - strand, within IAI47_20195 at 273.679 kb on - strand, within IAI47_20195 at 273.749 kb on + strand, within IAI47_20195 at 273.749 kb on + strand, within IAI47_20195 at 273.750 kb on - strand, within IAI47_20195 at 273.750 kb on - strand, within IAI47_20195 at 273.750 kb on - strand, within IAI47_20195 at 273.750 kb on - strand, within IAI47_20195 at 273.750 kb on - strand, within IAI47_20195 at 273.810 kb on + strand, within IAI47_20195 at 273.830 kb on + strand, within IAI47_20195 at 273.830 kb on + strand, within IAI47_20195 at 273.830 kb on + strand, within IAI47_20195 at 273.831 kb on - strand, within IAI47_20195 at 273.831 kb on - strand, within IAI47_20195 at 273.831 kb on - strand, within IAI47_20195 at 273.831 kb on - strand, within IAI47_20195 at 273.839 kb on + strand, within IAI47_20195 at 273.839 kb on + strand, within IAI47_20195 at 273.840 kb on - strand, within IAI47_20195 at 274.012 kb on + strand at 274.012 kb on + strand at 274.012 kb on + strand at 274.012 kb on + strand at 274.013 kb on - strand at 274.013 kb on - strand at 274.013 kb on - strand at 274.013 kb on - strand at 274.065 kb on + strand at 274.076 kb on + strand at 274.076 kb on + strand at 274.113 kb on + strand at 274.113 kb on + strand at 274.113 kb on + strand at 274.113 kb on + strand at 274.113 kb on + strand at 274.114 kb on - strand at 274.114 kb on - strand at 274.118 kb on + strand at 274.118 kb on + strand at 274.118 kb on + strand at 274.124 kb on + strand at 274.124 kb on + strand at 274.124 kb on + strand at 274.124 kb on + strand at 274.124 kb on + strand at 274.124 kb on + strand at 274.124 kb on + strand at 274.125 kb on - strand at 274.125 kb on - strand at 274.125 kb on - strand at 274.125 kb on - strand at 274.125 kb on - strand at 274.125 kb on - strand at 274.125 kb on - strand at 274.125 kb on - strand at 274.125 kb on - strand at 274.152 kb on + strand at 274.152 kb on + strand at 274.152 kb on + strand at 274.152 kb on + strand at 274.152 kb on + strand at 274.152 kb on + strand at 274.152 kb on + strand at 274.152 kb on + strand at 274.152 kb on + strand at 274.153 kb on - strand at 274.229 kb on + strand at 274.230 kb on - strand at 274.230 kb on - strand at 274.230 kb on - strand at 274.230 kb on - strand at 274.250 kb on + strand, within IAI47_20200 at 274.324 kb on + strand, within IAI47_20200 at 274.324 kb on + strand, within IAI47_20200 at 274.324 kb on + strand, within IAI47_20200 at 274.324 kb on + strand, within IAI47_20200 at 274.329 kb on + strand, within IAI47_20200 at 274.330 kb on - strand, within IAI47_20200 at 274.351 kb on + strand, within IAI47_20200 at 274.352 kb on - strand, within IAI47_20200 at 274.352 kb on - strand, within IAI47_20200 at 274.352 kb on - strand, within IAI47_20200 at 274.425 kb on + strand, within IAI47_20200 at 274.426 kb on - strand, within IAI47_20200 at 274.426 kb on - strand, within IAI47_20200 at 274.426 kb on - strand, within IAI47_20200 at 274.426 kb on - strand, within IAI47_20200 at 274.426 kb on - strand, within IAI47_20200 at 274.426 kb on - strand, within IAI47_20200 at 274.428 kb on + strand, within IAI47_20200 at 274.428 kb on + strand, within IAI47_20200 at 274.429 kb on - strand, within IAI47_20200 at 274.429 kb on - strand, within IAI47_20200 at 274.429 kb on - strand, within IAI47_20200 at 274.429 kb on - strand, within IAI47_20200 at 274.429 kb on - strand, within IAI47_20200 at 274.471 kb on + strand, within IAI47_20200 at 274.472 kb on - strand, within IAI47_20200 at 274.472 kb on - strand, within IAI47_20200 at 274.472 kb on - strand, within IAI47_20200 at 274.473 kb on + strand, within IAI47_20200 at 274.473 kb on + strand, within IAI47_20200 at 274.473 kb on + strand, within IAI47_20200 at 274.473 kb on + strand, within IAI47_20200 at 274.473 kb on + strand, within IAI47_20200 at 274.473 kb on + strand, within IAI47_20200 at 274.474 kb on - strand, within IAI47_20200 at 274.474 kb on - strand, within IAI47_20200 at 274.474 kb on - strand, within IAI47_20200 at 274.474 kb on - strand, within IAI47_20200 at 274.528 kb on + strand, within IAI47_20200 at 274.528 kb on + strand, within IAI47_20200 at 274.534 kb on + strand, within IAI47_20200 at 274.534 kb on + strand, within IAI47_20200 at 274.534 kb on + strand, within IAI47_20200 at 274.535 kb on - strand, within IAI47_20200 at 274.567 kb on + strand, within IAI47_20200 at 274.567 kb on + strand, within IAI47_20200 at 274.567 kb on + strand, within IAI47_20200 at 274.567 kb on + strand, within IAI47_20200 at 274.621 kb on - strand, within IAI47_20200 at 274.666 kb on + strand, within IAI47_20200 at 274.666 kb on + strand, within IAI47_20200 at 274.749 kb on + strand, within IAI47_20200 at 274.797 kb on + strand, within IAI47_20200 at 274.854 kb on - strand at 274.861 kb on + strand at 274.862 kb on - strand at 274.862 kb on - strand at 274.862 kb on - strand at 274.866 kb on + strand at 274.902 kb on + strand at 274.922 kb on + strand at 274.922 kb on + strand at 274.923 kb on - strand at 274.923 kb on - strand at 274.923 kb on - strand at 274.943 kb on + strand at 274.943 kb on + strand at 274.944 kb on - strand at 274.979 kb on + strand at 274.993 kb on - strand at 275.031 kb on + strand, within IAI47_20205
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 272,159 + IAI47_20190 0.75 +1.0 272,160 - IAI47_20190 0.75 -0.5 272,160 - IAI47_20190 0.75 +0.0 272,161 + IAI47_20190 0.75 +0.1 272,162 - IAI47_20190 0.75 -0.5 272,162 - IAI47_20190 0.75 -1.0 272,162 - IAI47_20190 0.75 -0.5 272,167 + IAI47_20190 0.76 -0.0 272,167 + IAI47_20190 0.76 -0.3 272,168 - IAI47_20190 0.76 +0.0 272,168 - IAI47_20190 0.76 +0.6 272,201 - IAI47_20190 0.76 +0.2 272,201 - IAI47_20190 0.76 +1.1 272,276 + IAI47_20190 0.79 +1.2 272,276 + IAI47_20190 0.79 +1.0 272,277 - IAI47_20190 0.79 -1.8 272,303 + IAI47_20190 0.79 -0.3 272,304 - IAI47_20190 0.79 -0.6 272,304 - IAI47_20190 0.79 +0.7 272,304 - IAI47_20190 0.79 -1.2 272,304 - IAI47_20190 0.79 +1.0 272,363 + IAI47_20190 0.81 +1.6 272,363 + IAI47_20190 0.81 -1.1 272,363 + IAI47_20190 0.81 -0.1 272,364 - IAI47_20190 0.81 +1.8 272,364 - IAI47_20190 0.81 +0.1 272,364 - IAI47_20190 0.81 +0.5 272,364 - IAI47_20190 0.81 -0.5 272,365 + IAI47_20190 0.81 -0.8 272,365 + IAI47_20190 0.81 -1.3 272,417 + IAI47_20190 0.82 +0.4 272,429 + IAI47_20190 0.83 +1.1 272,529 + IAI47_20190 0.86 +0.2 272,529 + IAI47_20190 0.86 -0.8 272,529 + IAI47_20190 0.86 -1.4 272,530 - IAI47_20190 0.86 -1.1 272,627 - IAI47_20190 0.88 -0.2 272,657 + IAI47_20190 0.89 +0.5 272,658 - IAI47_20190 0.89 -0.7 272,658 - IAI47_20190 0.89 +0.6 272,690 - -0.6 272,690 - -0.7 272,859 + +1.6 272,868 + +1.1 272,868 + -0.4 272,869 - -0.8 272,869 - +3.8 272,965 + +0.7 272,965 + +0.4 272,965 + +0.5 272,966 - +3.1 272,966 - +0.1 272,985 + +0.4 272,985 + +3.1 272,985 + -0.6 272,986 - +1.8 273,047 + +0.3 273,047 + -0.0 273,048 - -0.9 273,057 + +2.1 273,057 + -0.1 273,057 + +0.5 273,057 + +1.7 273,057 + +1.6 273,057 + +0.6 273,059 + +0.2 273,059 + -1.2 273,059 + +1.6 273,059 + +0.8 273,059 + +1.3 273,059 + -0.1 273,059 + +2.0 273,060 - -0.4 273,060 - -1.0 273,060 - -1.0 273,060 - +1.4 273,064 + +1.1 273,065 - +0.6 273,065 - +1.1 273,072 + +1.0 273,072 + -1.0 273,072 + +1.7 273,072 + +1.8 273,073 - +1.8 273,073 - +0.3 273,073 - -0.0 273,110 + +0.1 273,110 + +1.5 273,111 - -0.0 273,111 - +0.8 273,133 + +1.8 273,133 + +1.7 273,133 + -0.7 273,134 - -1.3 273,236 + -1.4 273,237 - +1.0 273,237 - +2.0 273,272 - IAI47_20195 0.14 +1.0 273,376 - IAI47_20195 0.25 +1.8 273,381 + IAI47_20195 0.26 -0.0 273,381 + IAI47_20195 0.26 +0.5 273,382 - IAI47_20195 0.26 +0.1 273,664 + IAI47_20195 0.57 -0.1 273,664 + IAI47_20195 0.57 +2.2 273,664 + IAI47_20195 0.57 -0.7 273,665 - IAI47_20195 0.57 +1.0 273,665 - IAI47_20195 0.57 +1.2 273,665 - IAI47_20195 0.57 +1.3 273,679 - IAI47_20195 0.59 +0.3 273,749 + IAI47_20195 0.66 +1.2 273,749 + IAI47_20195 0.66 +2.2 273,750 - IAI47_20195 0.66 +1.1 273,750 - IAI47_20195 0.66 +0.8 273,750 - IAI47_20195 0.66 +1.8 273,750 - IAI47_20195 0.66 -2.0 273,750 - IAI47_20195 0.66 +0.5 273,810 + IAI47_20195 0.73 +0.2 273,830 + IAI47_20195 0.75 -1.0 273,830 + IAI47_20195 0.75 +0.4 273,830 + IAI47_20195 0.75 +0.8 273,831 - IAI47_20195 0.75 -1.1 273,831 - IAI47_20195 0.75 -1.0 273,831 - IAI47_20195 0.75 +1.1 273,831 - IAI47_20195 0.75 +1.9 273,839 + IAI47_20195 0.76 +1.3 273,839 + IAI47_20195 0.76 -0.5 273,840 - IAI47_20195 0.76 +3.4 274,012 + -0.5 274,012 + +0.3 274,012 + -1.2 274,012 + +0.2 274,013 - -0.1 274,013 - +2.5 274,013 - -0.5 274,013 - +2.8 274,065 + +1.6 274,076 + -0.9 274,076 + +1.1 274,113 + +0.0 274,113 + -0.3 274,113 + +0.1 274,113 + +0.6 274,113 + +2.8 274,114 - +0.8 274,114 - +0.2 274,118 + +0.6 274,118 + -0.0 274,118 + +0.5 274,124 + +0.2 274,124 + -0.1 274,124 + +1.7 274,124 + -0.5 274,124 + -0.9 274,124 + -0.1 274,124 + +1.2 274,125 - +0.3 274,125 - -1.1 274,125 - +1.6 274,125 - +1.2 274,125 - -0.5 274,125 - +1.1 274,125 - -0.3 274,125 - +0.9 274,125 - -0.0 274,152 + +1.3 274,152 + +1.3 274,152 + +2.1 274,152 + +0.5 274,152 + +1.2 274,152 + +0.2 274,152 + +1.6 274,152 + -1.0 274,152 + +3.2 274,153 - -0.7 274,229 + -0.3 274,230 - -1.0 274,230 - +0.2 274,230 - +0.1 274,230 - +2.5 274,250 + IAI47_20200 0.13 +0.2 274,324 + IAI47_20200 0.22 -0.3 274,324 + IAI47_20200 0.22 +0.5 274,324 + IAI47_20200 0.22 +1.8 274,324 + IAI47_20200 0.22 -0.0 274,329 + IAI47_20200 0.23 -0.4 274,330 - IAI47_20200 0.23 -0.9 274,351 + IAI47_20200 0.26 +0.9 274,352 - IAI47_20200 0.26 +1.3 274,352 - IAI47_20200 0.26 -0.5 274,352 - IAI47_20200 0.26 +0.6 274,425 + IAI47_20200 0.35 +2.0 274,426 - IAI47_20200 0.35 -1.3 274,426 - IAI47_20200 0.35 -1.1 274,426 - IAI47_20200 0.35 -1.0 274,426 - IAI47_20200 0.35 +0.5 274,426 - IAI47_20200 0.35 +1.0 274,426 - IAI47_20200 0.35 +1.5 274,428 + IAI47_20200 0.36 -1.2 274,428 + IAI47_20200 0.36 -0.7 274,429 - IAI47_20200 0.36 +0.2 274,429 - IAI47_20200 0.36 +0.2 274,429 - IAI47_20200 0.36 -0.6 274,429 - IAI47_20200 0.36 +0.1 274,429 - IAI47_20200 0.36 -0.9 274,471 + IAI47_20200 0.41 +0.6 274,472 - IAI47_20200 0.41 +1.9 274,472 - IAI47_20200 0.41 -1.5 274,472 - IAI47_20200 0.41 +0.7 274,473 + IAI47_20200 0.41 +0.3 274,473 + IAI47_20200 0.41 +1.0 274,473 + IAI47_20200 0.41 -1.3 274,473 + IAI47_20200 0.41 -1.3 274,473 + IAI47_20200 0.41 +0.3 274,473 + IAI47_20200 0.41 -0.2 274,474 - IAI47_20200 0.41 +3.2 274,474 - IAI47_20200 0.41 -0.5 274,474 - IAI47_20200 0.41 +1.0 274,474 - IAI47_20200 0.41 -1.4 274,528 + IAI47_20200 0.48 +2.8 274,528 + IAI47_20200 0.48 -0.0 274,534 + IAI47_20200 0.49 +1.3 274,534 + IAI47_20200 0.49 +1.2 274,534 + IAI47_20200 0.49 +0.5 274,535 - IAI47_20200 0.49 +0.5 274,567 + IAI47_20200 0.53 +0.2 274,567 + IAI47_20200 0.53 +0.9 274,567 + IAI47_20200 0.53 +0.6 274,567 + IAI47_20200 0.53 +2.6 274,621 - IAI47_20200 0.60 +0.3 274,666 + IAI47_20200 0.66 +0.1 274,666 + IAI47_20200 0.66 -0.7 274,749 + IAI47_20200 0.77 -0.1 274,797 + IAI47_20200 0.83 +0.4 274,854 - +1.2 274,861 + +0.7 274,862 - +0.2 274,862 - +1.8 274,862 - -0.5 274,866 + -0.7 274,902 + +2.1 274,922 + +0.6 274,922 + +0.8 274,923 - +0.3 274,923 - +1.3 274,923 - -0.3 274,943 + +0.1 274,943 + -0.0 274,944 - +1.0 274,979 + +5.8 274,993 - +1.4 275,031 + IAI47_20205 0.11 +0.5
Or see this region's nucleotide sequence