Experiment: Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_15645 and IAI47_15650 overlap by 4 nucleotides IAI47_15650 and IAI47_15655 are separated by 83 nucleotides IAI47_15655 and IAI47_15660 are separated by 15 nucleotides IAI47_15660 and IAI47_15665 are separated by 47 nucleotides
IAI47_15645: IAI47_15645 - polynucleotide adenylyltransferase PcnB, at 3,299,813 to 3,301,252
_15645
IAI47_15650: IAI47_15650 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 3,301,249 to 3,301,734
_15650
IAI47_15655: IAI47_15655 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 3,301,818 to 3,302,612
_15655
IAI47_15660: IAI47_15660 - pantoate--beta-alanine ligase, at 3,302,628 to 3,303,482
_15660
IAI47_15665: IAI47_15665 - aspartate 1-decarboxylase, at 3,303,530 to 3,303,910
_15665
Position (kb)
3301
3302
3303 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3300.863 kb on + strand, within IAI47_15645 at 3300.895 kb on + strand, within IAI47_15645 at 3300.937 kb on + strand, within IAI47_15645 at 3300.937 kb on + strand, within IAI47_15645 at 3300.937 kb on + strand, within IAI47_15645 at 3300.940 kb on + strand, within IAI47_15645 at 3300.940 kb on + strand, within IAI47_15645 at 3300.949 kb on + strand, within IAI47_15645 at 3300.975 kb on + strand, within IAI47_15645 at 3300.975 kb on + strand, within IAI47_15645 at 3300.975 kb on + strand, within IAI47_15645 at 3300.975 kb on + strand, within IAI47_15645 at 3300.975 kb on + strand, within IAI47_15645 at 3301.043 kb on + strand, within IAI47_15645 at 3301.043 kb on + strand, within IAI47_15645 at 3301.043 kb on + strand, within IAI47_15645 at 3301.078 kb on + strand, within IAI47_15645 at 3301.078 kb on + strand, within IAI47_15645 at 3301.078 kb on + strand, within IAI47_15645 at 3301.159 kb on + strand at 3301.189 kb on + strand at 3301.189 kb on + strand at 3301.773 kb on + strand at 3301.781 kb on - strand at 3301.781 kb on - strand at 3301.890 kb on + strand at 3301.890 kb on + strand at 3301.928 kb on + strand, within IAI47_15655 at 3301.928 kb on + strand, within IAI47_15655 at 3301.928 kb on + strand, within IAI47_15655 at 3301.928 kb on + strand, within IAI47_15655 at 3301.929 kb on - strand, within IAI47_15655 at 3301.929 kb on - strand, within IAI47_15655 at 3301.929 kb on - strand, within IAI47_15655 at 3301.929 kb on - strand, within IAI47_15655 at 3302.016 kb on + strand, within IAI47_15655 at 3302.016 kb on + strand, within IAI47_15655 at 3302.016 kb on + strand, within IAI47_15655 at 3302.017 kb on - strand, within IAI47_15655 at 3302.017 kb on - strand, within IAI47_15655 at 3302.033 kb on - strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.078 kb on + strand, within IAI47_15655 at 3302.079 kb on - strand, within IAI47_15655 at 3302.079 kb on - strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.085 kb on + strand, within IAI47_15655 at 3302.086 kb on - strand, within IAI47_15655 at 3302.086 kb on - strand, within IAI47_15655 at 3302.086 kb on - strand, within IAI47_15655 at 3302.086 kb on - strand, within IAI47_15655 at 3302.144 kb on + strand, within IAI47_15655 at 3302.145 kb on - strand, within IAI47_15655 at 3302.145 kb on - strand, within IAI47_15655 at 3302.145 kb on - strand, within IAI47_15655 at 3302.253 kb on - strand, within IAI47_15655 at 3302.265 kb on + strand, within IAI47_15655 at 3302.267 kb on + strand, within IAI47_15655 at 3302.267 kb on + strand, within IAI47_15655 at 3302.267 kb on + strand, within IAI47_15655 at 3302.268 kb on - strand, within IAI47_15655 at 3302.268 kb on - strand, within IAI47_15655 at 3302.268 kb on - strand, within IAI47_15655 at 3302.272 kb on + strand, within IAI47_15655 at 3302.273 kb on - strand, within IAI47_15655 at 3302.273 kb on - strand, within IAI47_15655 at 3302.353 kb on + strand, within IAI47_15655 at 3302.353 kb on + strand, within IAI47_15655 at 3302.354 kb on - strand, within IAI47_15655 at 3302.391 kb on - strand, within IAI47_15655 at 3302.407 kb on + strand, within IAI47_15655 at 3302.556 kb on + strand at 3302.556 kb on + strand at 3302.556 kb on + strand at 3302.556 kb on + strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.557 kb on - strand at 3302.559 kb on - strand at 3302.579 kb on + strand at 3302.579 kb on + strand at 3302.580 kb on - strand at 3302.580 kb on - strand at 3303.041 kb on + strand, within IAI47_15660 at 3303.041 kb on + strand, within IAI47_15660 at 3303.041 kb on + strand, within IAI47_15660 at 3303.042 kb on - strand, within IAI47_15660 at 3303.042 kb on - strand, within IAI47_15660 at 3303.042 kb on - strand, within IAI47_15660 at 3303.060 kb on - strand, within IAI47_15660 at 3303.107 kb on + strand, within IAI47_15660 at 3303.107 kb on + strand, within IAI47_15660 at 3303.107 kb on + strand, within IAI47_15660 at 3303.108 kb on - strand, within IAI47_15660 at 3303.108 kb on - strand, within IAI47_15660 at 3303.221 kb on + strand, within IAI47_15660 at 3303.221 kb on + strand, within IAI47_15660 at 3303.222 kb on - strand, within IAI47_15660 at 3303.222 kb on - strand, within IAI47_15660 at 3303.222 kb on - strand, within IAI47_15660 at 3303.222 kb on - strand, within IAI47_15660 at 3303.247 kb on + strand, within IAI47_15660 at 3303.247 kb on + strand, within IAI47_15660 at 3303.248 kb on - strand, within IAI47_15660 at 3303.248 kb on - strand, within IAI47_15660 at 3303.415 kb on + strand at 3303.437 kb on + strand at 3303.480 kb on + strand at 3303.480 kb on + strand at 3303.480 kb on + strand at 3303.480 kb on + strand at 3303.480 kb on + strand at 3303.480 kb on + strand at 3303.481 kb on - strand at 3303.481 kb on - strand at 3303.505 kb on + strand at 3303.505 kb on + strand at 3303.505 kb on + strand at 3303.530 kb on - strand at 3303.593 kb on + strand, within IAI47_15665 at 3303.593 kb on + strand, within IAI47_15665 at 3303.593 kb on + strand, within IAI47_15665 at 3303.594 kb on - strand, within IAI47_15665 at 3303.594 kb on - strand, within IAI47_15665 at 3303.594 kb on - strand, within IAI47_15665 at 3303.594 kb on - strand, within IAI47_15665 at 3303.594 kb on - strand, within IAI47_15665 at 3303.610 kb on + strand, within IAI47_15665
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 3,300,863 + IAI47_15645 0.73 -2.6 3,300,895 + IAI47_15645 0.75 -1.5 3,300,937 + IAI47_15645 0.78 -0.2 3,300,937 + IAI47_15645 0.78 +0.4 3,300,937 + IAI47_15645 0.78 +3.1 3,300,940 + IAI47_15645 0.78 +0.4 3,300,940 + IAI47_15645 0.78 +0.6 3,300,949 + IAI47_15645 0.79 -0.3 3,300,975 + IAI47_15645 0.81 +2.1 3,300,975 + IAI47_15645 0.81 -1.2 3,300,975 + IAI47_15645 0.81 +0.4 3,300,975 + IAI47_15645 0.81 +1.6 3,300,975 + IAI47_15645 0.81 -0.7 3,301,043 + IAI47_15645 0.85 +4.5 3,301,043 + IAI47_15645 0.85 +0.8 3,301,043 + IAI47_15645 0.85 +0.8 3,301,078 + IAI47_15645 0.88 +4.9 3,301,078 + IAI47_15645 0.88 +3.2 3,301,078 + IAI47_15645 0.88 +1.4 3,301,159 + -0.9 3,301,189 + +0.6 3,301,189 + +0.8 3,301,773 + +1.0 3,301,781 - -0.0 3,301,781 - -0.1 3,301,890 + +0.5 3,301,890 + -2.5 3,301,928 + IAI47_15655 0.14 +0.2 3,301,928 + IAI47_15655 0.14 -1.2 3,301,928 + IAI47_15655 0.14 +0.8 3,301,928 + IAI47_15655 0.14 -2.1 3,301,929 - IAI47_15655 0.14 -1.3 3,301,929 - IAI47_15655 0.14 -0.2 3,301,929 - IAI47_15655 0.14 -0.4 3,301,929 - IAI47_15655 0.14 -0.0 3,302,016 + IAI47_15655 0.25 -1.0 3,302,016 + IAI47_15655 0.25 +0.5 3,302,016 + IAI47_15655 0.25 +1.1 3,302,017 - IAI47_15655 0.25 -2.7 3,302,017 - IAI47_15655 0.25 -0.9 3,302,033 - IAI47_15655 0.27 +1.8 3,302,078 + IAI47_15655 0.33 -2.2 3,302,078 + IAI47_15655 0.33 -1.3 3,302,078 + IAI47_15655 0.33 -0.7 3,302,078 + IAI47_15655 0.33 -1.4 3,302,078 + IAI47_15655 0.33 +0.5 3,302,078 + IAI47_15655 0.33 +2.0 3,302,078 + IAI47_15655 0.33 -0.0 3,302,078 + IAI47_15655 0.33 -0.2 3,302,079 - IAI47_15655 0.33 -0.7 3,302,079 - IAI47_15655 0.33 +0.8 3,302,085 + IAI47_15655 0.34 +0.2 3,302,085 + IAI47_15655 0.34 -1.1 3,302,085 + IAI47_15655 0.34 +0.1 3,302,085 + IAI47_15655 0.34 +0.4 3,302,085 + IAI47_15655 0.34 +0.0 3,302,085 + IAI47_15655 0.34 +4.3 3,302,085 + IAI47_15655 0.34 -0.9 3,302,086 - IAI47_15655 0.34 -2.1 3,302,086 - IAI47_15655 0.34 -0.7 3,302,086 - IAI47_15655 0.34 +4.9 3,302,086 - IAI47_15655 0.34 +0.5 3,302,144 + IAI47_15655 0.41 +1.3 3,302,145 - IAI47_15655 0.41 +1.0 3,302,145 - IAI47_15655 0.41 -0.8 3,302,145 - IAI47_15655 0.41 -0.3 3,302,253 - IAI47_15655 0.55 +2.0 3,302,265 + IAI47_15655 0.56 +2.4 3,302,267 + IAI47_15655 0.56 +3.5 3,302,267 + IAI47_15655 0.56 -2.0 3,302,267 + IAI47_15655 0.56 -0.0 3,302,268 - IAI47_15655 0.57 -2.3 3,302,268 - IAI47_15655 0.57 -0.0 3,302,268 - IAI47_15655 0.57 -2.0 3,302,272 + IAI47_15655 0.57 -0.1 3,302,273 - IAI47_15655 0.57 +0.3 3,302,273 - IAI47_15655 0.57 -0.9 3,302,353 + IAI47_15655 0.67 +1.6 3,302,353 + IAI47_15655 0.67 -1.5 3,302,354 - IAI47_15655 0.67 -1.5 3,302,391 - IAI47_15655 0.72 +0.1 3,302,407 + IAI47_15655 0.74 -2.3 3,302,556 + -0.9 3,302,556 + +2.0 3,302,556 + +0.0 3,302,556 + -0.5 3,302,557 - -2.0 3,302,557 - -0.4 3,302,557 - +0.3 3,302,557 - +0.8 3,302,557 - +0.3 3,302,557 - -1.4 3,302,557 - +0.3 3,302,557 - -2.4 3,302,557 - +0.5 3,302,559 - -0.8 3,302,579 + +1.4 3,302,579 + +1.5 3,302,580 - -1.4 3,302,580 - -1.7 3,303,041 + IAI47_15660 0.48 +0.4 3,303,041 + IAI47_15660 0.48 -0.2 3,303,041 + IAI47_15660 0.48 -1.0 3,303,042 - IAI47_15660 0.48 +0.6 3,303,042 - IAI47_15660 0.48 +0.5 3,303,042 - IAI47_15660 0.48 -1.0 3,303,060 - IAI47_15660 0.51 +0.7 3,303,107 + IAI47_15660 0.56 -1.7 3,303,107 + IAI47_15660 0.56 +2.6 3,303,107 + IAI47_15660 0.56 +0.2 3,303,108 - IAI47_15660 0.56 -0.6 3,303,108 - IAI47_15660 0.56 +1.7 3,303,221 + IAI47_15660 0.69 -1.3 3,303,221 + IAI47_15660 0.69 -0.5 3,303,222 - IAI47_15660 0.69 -0.4 3,303,222 - IAI47_15660 0.69 -1.1 3,303,222 - IAI47_15660 0.69 -0.2 3,303,222 - IAI47_15660 0.69 -1.5 3,303,247 + IAI47_15660 0.72 +1.1 3,303,247 + IAI47_15660 0.72 +1.6 3,303,248 - IAI47_15660 0.73 -2.7 3,303,248 - IAI47_15660 0.73 -1.2 3,303,415 + +2.1 3,303,437 + +0.3 3,303,480 + +3.6 3,303,480 + +0.2 3,303,480 + +0.6 3,303,480 + -0.5 3,303,480 + +1.9 3,303,480 + +1.5 3,303,481 - -1.0 3,303,481 - +0.4 3,303,505 + -1.1 3,303,505 + +0.3 3,303,505 + -0.3 3,303,530 - -1.8 3,303,593 + IAI47_15665 0.17 -0.3 3,303,593 + IAI47_15665 0.17 +0.1 3,303,593 + IAI47_15665 0.17 -2.7 3,303,594 - IAI47_15665 0.17 -1.0 3,303,594 - IAI47_15665 0.17 -1.0 3,303,594 - IAI47_15665 0.17 +0.3 3,303,594 - IAI47_15665 0.17 -1.1 3,303,594 - IAI47_15665 0.17 -1.3 3,303,610 + IAI47_15665 0.21 -0.4
Or see this region's nucleotide sequence