Experiment: Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_03560 and IAI47_03565 are separated by 154 nucleotides IAI47_03565 and IAI47_03570 overlap by 1 nucleotides IAI47_03570 and IAI47_03575 are separated by 65 nucleotides
IAI47_03560: IAI47_03560 - A/G-specific adenine glycosylase, at 744,159 to 745,244
_03560
IAI47_03565: IAI47_03565 - tRNA (guanosine(46)-N7)-methyltransferase TrmB, at 745,399 to 746,118
_03565
IAI47_03570: IAI47_03570 - YggL family protein, at 746,118 to 746,444
_03570
IAI47_03575: IAI47_03575 - DUF2884 domain-containing protein, at 746,510 to 747,226
_03575
Position (kb) 745
746
747 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 744.569 kb on + strand, within IAI47_03560 at 744.569 kb on + strand, within IAI47_03560 at 744.570 kb on - strand, within IAI47_03560 at 744.570 kb on - strand, within IAI47_03560 at 744.586 kb on + strand, within IAI47_03560 at 744.610 kb on + strand, within IAI47_03560 at 744.611 kb on - strand, within IAI47_03560 at 744.611 kb on - strand, within IAI47_03560 at 744.629 kb on + strand, within IAI47_03560 at 744.630 kb on - strand, within IAI47_03560 at 744.630 kb on - strand, within IAI47_03560 at 744.630 kb on - strand, within IAI47_03560 at 744.630 kb on - strand, within IAI47_03560 at 744.701 kb on + strand, within IAI47_03560 at 744.702 kb on - strand, within IAI47_03560 at 744.797 kb on - strand, within IAI47_03560 at 744.923 kb on + strand, within IAI47_03560 at 744.924 kb on - strand, within IAI47_03560 at 744.972 kb on - strand, within IAI47_03560 at 744.972 kb on - strand, within IAI47_03560 at 744.972 kb on - strand, within IAI47_03560 at 744.972 kb on - strand, within IAI47_03560 at 744.981 kb on - strand, within IAI47_03560 at 744.981 kb on - strand, within IAI47_03560 at 744.994 kb on + strand, within IAI47_03560 at 744.994 kb on + strand, within IAI47_03560 at 744.995 kb on - strand, within IAI47_03560 at 744.995 kb on - strand, within IAI47_03560 at 744.995 kb on - strand, within IAI47_03560 at 744.995 kb on - strand, within IAI47_03560 at 744.997 kb on - strand, within IAI47_03560 at 744.998 kb on - strand, within IAI47_03560 at 745.076 kb on + strand, within IAI47_03560 at 745.077 kb on - strand, within IAI47_03560 at 745.077 kb on - strand, within IAI47_03560 at 745.090 kb on + strand, within IAI47_03560 at 745.090 kb on + strand, within IAI47_03560 at 745.098 kb on + strand, within IAI47_03560 at 745.098 kb on + strand, within IAI47_03560 at 745.122 kb on + strand, within IAI47_03560 at 745.122 kb on + strand, within IAI47_03560 at 745.122 kb on + strand, within IAI47_03560 at 745.122 kb on + strand, within IAI47_03560 at 745.123 kb on - strand, within IAI47_03560 at 745.123 kb on - strand, within IAI47_03560 at 745.148 kb on + strand at 745.148 kb on + strand at 745.148 kb on + strand at 745.148 kb on + strand at 745.148 kb on + strand at 745.148 kb on + strand at 745.148 kb on + strand at 745.148 kb on + strand at 745.148 kb on + strand at 745.149 kb on - strand at 745.149 kb on - strand at 745.149 kb on - strand at 745.149 kb on - strand at 745.149 kb on - strand at 745.149 kb on - strand at 745.149 kb on - strand at 745.149 kb on - strand at 745.149 kb on - strand at 745.149 kb on - strand at 745.149 kb on - strand at 745.150 kb on + strand at 745.150 kb on + strand at 745.150 kb on + strand at 745.150 kb on + strand at 745.150 kb on + strand at 745.150 kb on + strand at 745.151 kb on - strand at 745.151 kb on - strand at 745.151 kb on - strand at 745.151 kb on - strand at 745.151 kb on - strand at 745.151 kb on - strand at 745.151 kb on - strand at 745.151 kb on - strand at 745.202 kb on - strand at 745.244 kb on + strand at 745.244 kb on + strand at 745.244 kb on + strand at 745.244 kb on + strand at 745.244 kb on + strand at 745.244 kb on + strand at 745.244 kb on + strand at 745.244 kb on + strand at 745.244 kb on + strand at 745.244 kb on + strand at 745.245 kb on - strand at 745.245 kb on - strand at 745.245 kb on - strand at 745.245 kb on - strand at 745.245 kb on - strand at 745.266 kb on + strand at 745.266 kb on + strand at 745.266 kb on + strand at 745.266 kb on + strand at 745.266 kb on + strand at 745.267 kb on - strand at 745.271 kb on + strand at 745.271 kb on + strand at 745.271 kb on + strand at 745.271 kb on + strand at 745.272 kb on - strand at 745.272 kb on - strand at 745.311 kb on + strand at 745.311 kb on + strand at 745.312 kb on - strand at 745.312 kb on - strand at 745.312 kb on - strand at 745.344 kb on + strand at 745.344 kb on + strand at 745.344 kb on + strand at 745.345 kb on - strand at 745.345 kb on - strand at 745.357 kb on + strand at 745.357 kb on + strand at 745.357 kb on + strand at 745.358 kb on - strand at 745.382 kb on - strand at 745.382 kb on - strand at 745.396 kb on + strand at 745.396 kb on + strand at 745.396 kb on + strand at 745.397 kb on - strand at 745.397 kb on - strand at 745.397 kb on - strand at 745.404 kb on + strand at 745.404 kb on + strand at 745.405 kb on - strand at 745.405 kb on - strand at 745.405 kb on - strand at 745.461 kb on + strand at 745.461 kb on + strand at 745.543 kb on + strand, within IAI47_03565 at 745.543 kb on + strand, within IAI47_03565 at 745.544 kb on - strand, within IAI47_03565 at 745.638 kb on + strand, within IAI47_03565 at 745.638 kb on + strand, within IAI47_03565 at 745.697 kb on + strand, within IAI47_03565 at 745.697 kb on + strand, within IAI47_03565 at 745.697 kb on + strand, within IAI47_03565 at 745.697 kb on + strand, within IAI47_03565 at 745.697 kb on + strand, within IAI47_03565 at 745.697 kb on + strand, within IAI47_03565 at 745.698 kb on - strand, within IAI47_03565 at 745.698 kb on - strand, within IAI47_03565 at 745.698 kb on - strand, within IAI47_03565 at 745.698 kb on - strand, within IAI47_03565 at 745.807 kb on - strand, within IAI47_03565 at 745.839 kb on + strand, within IAI47_03565 at 745.839 kb on + strand, within IAI47_03565 at 745.839 kb on + strand, within IAI47_03565 at 745.839 kb on + strand, within IAI47_03565 at 745.839 kb on + strand, within IAI47_03565 at 745.840 kb on - strand, within IAI47_03565 at 745.840 kb on - strand, within IAI47_03565 at 745.855 kb on - strand, within IAI47_03565 at 745.855 kb on - strand, within IAI47_03565 at 745.899 kb on + strand, within IAI47_03565 at 745.900 kb on - strand, within IAI47_03565 at 745.900 kb on - strand, within IAI47_03565 at 745.900 kb on - strand, within IAI47_03565 at 745.900 kb on - strand, within IAI47_03565 at 745.900 kb on - strand, within IAI47_03565 at 745.900 kb on - strand, within IAI47_03565 at 745.900 kb on - strand, within IAI47_03565 at 745.949 kb on - strand, within IAI47_03565 at 745.949 kb on - strand, within IAI47_03565 at 745.949 kb on - strand, within IAI47_03565 at 745.949 kb on - strand, within IAI47_03565 at 745.959 kb on + strand, within IAI47_03565 at 745.959 kb on + strand, within IAI47_03565 at 745.959 kb on + strand, within IAI47_03565 at 745.959 kb on + strand, within IAI47_03565 at 745.959 kb on + strand, within IAI47_03565 at 745.959 kb on + strand, within IAI47_03565 at 745.959 kb on + strand, within IAI47_03565 at 745.960 kb on - strand, within IAI47_03565 at 745.960 kb on - strand, within IAI47_03565 at 745.960 kb on - strand, within IAI47_03565 at 746.017 kb on + strand, within IAI47_03565 at 746.017 kb on + strand, within IAI47_03565 at 746.017 kb on + strand, within IAI47_03565 at 746.017 kb on + strand, within IAI47_03565 at 746.017 kb on + strand, within IAI47_03565 at 746.017 kb on + strand, within IAI47_03565 at 746.018 kb on - strand, within IAI47_03565 at 746.018 kb on - strand, within IAI47_03565 at 746.018 kb on - strand, within IAI47_03565 at 746.075 kb on + strand at 746.076 kb on - strand at 746.088 kb on - strand at 746.096 kb on + strand at 746.096 kb on + strand at 746.096 kb on + strand at 746.097 kb on - strand at 746.116 kb on + strand at 746.116 kb on + strand at 746.302 kb on - strand, within IAI47_03570 at 746.302 kb on - strand, within IAI47_03570 at 746.342 kb on + strand, within IAI47_03570 at 746.343 kb on - strand, within IAI47_03570 at 746.348 kb on + strand, within IAI47_03570 at 746.348 kb on + strand, within IAI47_03570 at 746.348 kb on + strand, within IAI47_03570 at 746.349 kb on - strand, within IAI47_03570 at 746.442 kb on + strand at 746.442 kb on + strand at 746.442 kb on + strand at 746.443 kb on - strand at 746.443 kb on - strand at 746.443 kb on - strand at 746.498 kb on + strand at 746.499 kb on - strand at 746.505 kb on + strand at 746.505 kb on + strand at 746.505 kb on + strand at 746.505 kb on + strand at 746.505 kb on + strand at 746.505 kb on + strand at 746.505 kb on + strand at 746.506 kb on - strand at 746.506 kb on - strand at 746.506 kb on - strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.509 kb on + strand at 746.510 kb on - strand at 746.510 kb on - strand at 746.510 kb on - strand at 746.510 kb on - strand at 746.510 kb on - strand at 746.510 kb on - strand at 746.510 kb on - strand at 746.510 kb on - strand at 746.510 kb on - strand at 746.510 kb on - strand at 746.510 kb on - strand at 746.518 kb on + strand at 746.519 kb on - strand at 746.545 kb on - strand at 746.545 kb on - strand at 746.570 kb on + strand at 746.570 kb on + strand at 746.571 kb on - strand at 746.571 kb on - strand at 746.571 kb on - strand at 746.571 kb on - strand at 746.571 kb on - strand at 746.571 kb on - strand at 746.584 kb on + strand, within IAI47_03575 at 746.584 kb on + strand, within IAI47_03575 at 746.647 kb on + strand, within IAI47_03575 at 746.648 kb on - strand, within IAI47_03575 at 746.704 kb on + strand, within IAI47_03575 at 746.705 kb on - strand, within IAI47_03575 at 746.733 kb on - strand, within IAI47_03575 at 746.908 kb on + strand, within IAI47_03575 at 747.016 kb on + strand, within IAI47_03575 at 747.037 kb on + strand, within IAI47_03575 at 747.038 kb on - strand, within IAI47_03575 at 747.038 kb on - strand, within IAI47_03575 at 747.076 kb on + strand, within IAI47_03575 at 747.077 kb on - strand, within IAI47_03575 at 747.077 kb on - strand, within IAI47_03575
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 5.0 mM Glucose; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 744,569 + IAI47_03560 0.38 +0.1 744,569 + IAI47_03560 0.38 -0.9 744,570 - IAI47_03560 0.38 +1.5 744,570 - IAI47_03560 0.38 +0.6 744,586 + IAI47_03560 0.39 +0.8 744,610 + IAI47_03560 0.42 -2.6 744,611 - IAI47_03560 0.42 -2.1 744,611 - IAI47_03560 0.42 +0.6 744,629 + IAI47_03560 0.43 -0.4 744,630 - IAI47_03560 0.43 -1.1 744,630 - IAI47_03560 0.43 -1.1 744,630 - IAI47_03560 0.43 +0.2 744,630 - IAI47_03560 0.43 -0.5 744,701 + IAI47_03560 0.50 +0.0 744,702 - IAI47_03560 0.50 +0.7 744,797 - IAI47_03560 0.59 -0.6 744,923 + IAI47_03560 0.70 -0.7 744,924 - IAI47_03560 0.70 -1.9 744,972 - IAI47_03560 0.75 -0.3 744,972 - IAI47_03560 0.75 -1.3 744,972 - IAI47_03560 0.75 -1.0 744,972 - IAI47_03560 0.75 -0.8 744,981 - IAI47_03560 0.76 -0.5 744,981 - IAI47_03560 0.76 -0.8 744,994 + IAI47_03560 0.77 +0.0 744,994 + IAI47_03560 0.77 +0.0 744,995 - IAI47_03560 0.77 -0.5 744,995 - IAI47_03560 0.77 -0.0 744,995 - IAI47_03560 0.77 -0.2 744,995 - IAI47_03560 0.77 -0.4 744,997 - IAI47_03560 0.77 +0.5 744,998 - IAI47_03560 0.77 -0.4 745,076 + IAI47_03560 0.84 -0.9 745,077 - IAI47_03560 0.85 -2.5 745,077 - IAI47_03560 0.85 +1.4 745,090 + IAI47_03560 0.86 -0.2 745,090 + IAI47_03560 0.86 +4.1 745,098 + IAI47_03560 0.86 -1.1 745,098 + IAI47_03560 0.86 -1.3 745,122 + IAI47_03560 0.89 +0.3 745,122 + IAI47_03560 0.89 -0.6 745,122 + IAI47_03560 0.89 -0.5 745,122 + IAI47_03560 0.89 -0.7 745,123 - IAI47_03560 0.89 +0.4 745,123 - IAI47_03560 0.89 +1.2 745,148 + +0.1 745,148 + +2.1 745,148 + -1.3 745,148 + +0.3 745,148 + -1.6 745,148 + +2.4 745,148 + +0.1 745,148 + +1.2 745,148 + +0.3 745,149 - +0.5 745,149 - +3.1 745,149 - +1.8 745,149 - -1.0 745,149 - -0.5 745,149 - +3.2 745,149 - +0.5 745,149 - -1.2 745,149 - +1.6 745,149 - +0.0 745,149 - -0.7 745,150 + +0.5 745,150 + -1.6 745,150 + -0.3 745,150 + +0.7 745,150 + +0.9 745,150 + -0.1 745,151 - -0.1 745,151 - +1.4 745,151 - +0.9 745,151 - +1.6 745,151 - +3.4 745,151 - -0.1 745,151 - +0.1 745,151 - +0.2 745,202 - -1.9 745,244 + -0.4 745,244 + -0.5 745,244 + +0.3 745,244 + -1.7 745,244 + -1.0 745,244 + -2.2 745,244 + -3.0 745,244 + -0.4 745,244 + +0.1 745,244 + +4.2 745,245 - -0.2 745,245 - +0.7 745,245 - +1.2 745,245 - -0.3 745,245 - -0.2 745,266 + -0.9 745,266 + +1.4 745,266 + -0.7 745,266 + -1.4 745,266 + -1.0 745,267 - -0.3 745,271 + -0.9 745,271 + -2.9 745,271 + +0.9 745,271 + +0.3 745,272 - +1.6 745,272 - -0.1 745,311 + -2.3 745,311 + +1.4 745,312 - +0.9 745,312 - -0.5 745,312 - -1.7 745,344 + -0.2 745,344 + -0.7 745,344 + +4.8 745,345 - +0.1 745,345 - -0.2 745,357 + -0.7 745,357 + +1.5 745,357 + +0.0 745,358 - -0.6 745,382 - +0.3 745,382 - +0.7 745,396 + +1.9 745,396 + -0.6 745,396 + -0.5 745,397 - -2.2 745,397 - -0.6 745,397 - -0.8 745,404 + -0.4 745,404 + +5.7 745,405 - -1.6 745,405 - -0.1 745,405 - -0.6 745,461 + +0.7 745,461 + +2.9 745,543 + IAI47_03565 0.20 -0.4 745,543 + IAI47_03565 0.20 +3.4 745,544 - IAI47_03565 0.20 -0.5 745,638 + IAI47_03565 0.33 +0.8 745,638 + IAI47_03565 0.33 +4.7 745,697 + IAI47_03565 0.41 +0.6 745,697 + IAI47_03565 0.41 -0.0 745,697 + IAI47_03565 0.41 +0.7 745,697 + IAI47_03565 0.41 +0.4 745,697 + IAI47_03565 0.41 +0.6 745,697 + IAI47_03565 0.41 +0.7 745,698 - IAI47_03565 0.42 -0.9 745,698 - IAI47_03565 0.42 -1.4 745,698 - IAI47_03565 0.42 -2.0 745,698 - IAI47_03565 0.42 +0.6 745,807 - IAI47_03565 0.57 -0.4 745,839 + IAI47_03565 0.61 +0.8 745,839 + IAI47_03565 0.61 +3.2 745,839 + IAI47_03565 0.61 +4.8 745,839 + IAI47_03565 0.61 -1.4 745,839 + IAI47_03565 0.61 +0.7 745,840 - IAI47_03565 0.61 -3.2 745,840 - IAI47_03565 0.61 -0.3 745,855 - IAI47_03565 0.63 +0.8 745,855 - IAI47_03565 0.63 -0.4 745,899 + IAI47_03565 0.69 -0.5 745,900 - IAI47_03565 0.70 -0.8 745,900 - IAI47_03565 0.70 +0.8 745,900 - IAI47_03565 0.70 +0.5 745,900 - IAI47_03565 0.70 -1.0 745,900 - IAI47_03565 0.70 +1.2 745,900 - IAI47_03565 0.70 -0.3 745,900 - IAI47_03565 0.70 -0.0 745,949 - IAI47_03565 0.76 -1.4 745,949 - IAI47_03565 0.76 -0.0 745,949 - IAI47_03565 0.76 -1.5 745,949 - IAI47_03565 0.76 -1.7 745,959 + IAI47_03565 0.78 +0.7 745,959 + IAI47_03565 0.78 +0.8 745,959 + IAI47_03565 0.78 -1.8 745,959 + IAI47_03565 0.78 +0.8 745,959 + IAI47_03565 0.78 +1.7 745,959 + IAI47_03565 0.78 +0.1 745,959 + IAI47_03565 0.78 -0.7 745,960 - IAI47_03565 0.78 -1.7 745,960 - IAI47_03565 0.78 -1.0 745,960 - IAI47_03565 0.78 +0.7 746,017 + IAI47_03565 0.86 -2.0 746,017 + IAI47_03565 0.86 +0.4 746,017 + IAI47_03565 0.86 -0.6 746,017 + IAI47_03565 0.86 +2.6 746,017 + IAI47_03565 0.86 +0.8 746,017 + IAI47_03565 0.86 -0.1 746,018 - IAI47_03565 0.86 +0.4 746,018 - IAI47_03565 0.86 -0.1 746,018 - IAI47_03565 0.86 +0.0 746,075 + +2.0 746,076 - -1.2 746,088 - -2.2 746,096 + +0.4 746,096 + -0.4 746,096 + +2.5 746,097 - -1.4 746,116 + -0.6 746,116 + -0.2 746,302 - IAI47_03570 0.56 +0.1 746,302 - IAI47_03570 0.56 +0.1 746,342 + IAI47_03570 0.69 +0.4 746,343 - IAI47_03570 0.69 -1.9 746,348 + IAI47_03570 0.70 -1.3 746,348 + IAI47_03570 0.70 +1.9 746,348 + IAI47_03570 0.70 +1.7 746,349 - IAI47_03570 0.71 +0.8 746,442 + +0.8 746,442 + -0.8 746,442 + +1.6 746,443 - -0.2 746,443 - -2.1 746,443 - -1.2 746,498 + -0.3 746,499 - +0.6 746,505 + -1.3 746,505 + +1.9 746,505 + +1.1 746,505 + +0.2 746,505 + -0.5 746,505 + +1.0 746,505 + -0.8 746,506 - -1.4 746,506 - +1.2 746,506 - +0.3 746,509 + -1.3 746,509 + +0.0 746,509 + -0.2 746,509 + +0.1 746,509 + +0.6 746,509 + +0.6 746,509 + +2.2 746,509 + -0.7 746,509 + +0.5 746,509 + -0.5 746,509 + -0.6 746,509 + +1.8 746,509 + -0.4 746,509 + -0.7 746,509 + -0.1 746,509 + +0.4 746,509 + +1.6 746,509 + +0.4 746,510 - +0.1 746,510 - -2.6 746,510 - -1.1 746,510 - -2.8 746,510 - -3.3 746,510 - -0.7 746,510 - -0.8 746,510 - -1.1 746,510 - -2.2 746,510 - -0.7 746,510 - -1.0 746,518 + +1.1 746,519 - -1.3 746,545 - -0.7 746,545 - -1.9 746,570 + +1.3 746,570 + +0.7 746,571 - -2.4 746,571 - -3.0 746,571 - -1.1 746,571 - -1.2 746,571 - -2.1 746,571 - -2.2 746,584 + IAI47_03575 0.10 +0.8 746,584 + IAI47_03575 0.10 -0.4 746,647 + IAI47_03575 0.19 -1.2 746,648 - IAI47_03575 0.19 -0.9 746,704 + IAI47_03575 0.27 +0.2 746,705 - IAI47_03575 0.27 -1.7 746,733 - IAI47_03575 0.31 -1.2 746,908 + IAI47_03575 0.56 -0.2 747,016 + IAI47_03575 0.71 -0.7 747,037 + IAI47_03575 0.74 -1.2 747,038 - IAI47_03575 0.74 -1.9 747,038 - IAI47_03575 0.74 -0.7 747,076 + IAI47_03575 0.79 +0.7 747,077 - IAI47_03575 0.79 -1.9 747,077 - IAI47_03575 0.79 +0.4
Or see this region's nucleotide sequence