Experiment: L-Proline (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0215 and Psyr_0216 are separated by 82 nucleotides Psyr_0216 and Psyr_0217 are separated by 10 nucleotides Psyr_0217 and Psyr_0218 are separated by 331 nucleotides
Psyr_0215: Psyr_0215 - Exodeoxyribonuclease III xth, at 228,597 to 229,376
_0215
Psyr_0216: Psyr_0216 - orotate phosphoribosyltransferase, at 229,459 to 230,100
_0216
Psyr_0217: Psyr_0217 - conserved hypothetical protein, at 230,111 to 230,743
_0217
Psyr_0218: Psyr_0218 - N-acetylglutamate kinase, at 231,075 to 231,980
_0218
Position (kb) 230
231 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 229.166 kb on - strand, within Psyr_0215 at 229.166 kb on - strand, within Psyr_0215 at 229.210 kb on + strand, within Psyr_0215 at 229.210 kb on + strand, within Psyr_0215 at 229.211 kb on - strand, within Psyr_0215 at 229.219 kb on + strand, within Psyr_0215 at 229.219 kb on + strand, within Psyr_0215 at 229.219 kb on + strand, within Psyr_0215 at 229.220 kb on - strand, within Psyr_0215 at 229.220 kb on - strand, within Psyr_0215 at 229.278 kb on + strand, within Psyr_0215 at 229.278 kb on + strand, within Psyr_0215 at 229.353 kb on + strand at 229.353 kb on + strand at 229.354 kb on - strand at 229.354 kb on - strand at 229.354 kb on - strand at 229.354 kb on - strand at 229.376 kb on + strand at 229.403 kb on + strand at 229.403 kb on + strand at 229.403 kb on + strand at 229.403 kb on + strand at 229.404 kb on - strand at 229.404 kb on - strand at 229.420 kb on - strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.423 kb on + strand at 229.427 kb on + strand at 229.427 kb on + strand at 229.428 kb on - strand at 229.442 kb on + strand at 229.672 kb on + strand, within Psyr_0216 at 230.238 kb on - strand, within Psyr_0217 at 230.297 kb on + strand, within Psyr_0217 at 230.297 kb on + strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.313 kb on - strand, within Psyr_0217 at 230.365 kb on + strand, within Psyr_0217 at 230.438 kb on + strand, within Psyr_0217 at 230.439 kb on - strand, within Psyr_0217 at 230.839 kb on + strand at 231.043 kb on - strand at 231.065 kb on + strand at 231.066 kb on - strand at 231.109 kb on - strand at 231.109 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (C) remove 229,166 - Psyr_0215 0.73 +1.0 229,166 - Psyr_0215 0.73 +1.6 229,210 + Psyr_0215 0.79 +2.2 229,210 + Psyr_0215 0.79 +0.6 229,211 - Psyr_0215 0.79 -0.1 229,219 + Psyr_0215 0.80 -0.3 229,219 + Psyr_0215 0.80 -0.2 229,219 + Psyr_0215 0.80 -0.6 229,220 - Psyr_0215 0.80 -1.2 229,220 - Psyr_0215 0.80 +1.4 229,278 + Psyr_0215 0.87 -1.3 229,278 + Psyr_0215 0.87 -0.6 229,353 + +0.4 229,353 + -0.6 229,354 - -1.0 229,354 - -1.6 229,354 - +0.4 229,354 - -3.0 229,376 + +0.4 229,403 + -2.3 229,403 + +0.4 229,403 + +0.1 229,403 + -1.4 229,404 - +2.4 229,404 - -1.6 229,420 - -2.2 229,423 + -1.6 229,423 + -0.7 229,423 + +4.1 229,423 + +0.6 229,423 + +0.8 229,427 + -0.4 229,427 + +0.9 229,428 - +0.4 229,442 + -1.1 229,672 + Psyr_0216 0.33 +0.4 230,238 - Psyr_0217 0.20 -1.4 230,297 + Psyr_0217 0.29 -1.3 230,297 + Psyr_0217 0.29 -1.1 230,298 - Psyr_0217 0.30 -2.4 230,298 - Psyr_0217 0.30 -0.0 230,298 - Psyr_0217 0.30 +0.2 230,298 - Psyr_0217 0.30 -0.9 230,313 - Psyr_0217 0.32 +0.6 230,365 + Psyr_0217 0.40 -0.6 230,438 + Psyr_0217 0.52 -1.3 230,439 - Psyr_0217 0.52 +0.4 230,839 + +2.4 231,043 - -0.6 231,065 + +0.8 231,066 - -1.0 231,109 - +1.4 231,109 - +0.4
Or see this region's nucleotide sequence