Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26765 and BWI76_RS26770 are separated by 436 nucleotides BWI76_RS26770 and BWI76_RS26775 overlap by 19 nucleotides
BWI76_RS26765: BWI76_RS26765 - phosphodiesterase, at 5,543,932 to 5,545,938
_RS26765
BWI76_RS26770: BWI76_RS26770 - cellulose biosynthesis protein BcsC, at 5,546,375 to 5,549,860
_RS26770
BWI76_RS26775: BWI76_RS26775 - endo-1,4-D-glucanase, at 5,549,842 to 5,550,951
_RS26775
Position (kb) 5546
5547
5548
5549
5550 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5545.562 kb on - strand, within BWI76_RS26765 at 5545.675 kb on + strand, within BWI76_RS26765 at 5545.735 kb on - strand, within BWI76_RS26765 at 5545.735 kb on - strand, within BWI76_RS26765 at 5545.756 kb on - strand at 5545.795 kb on + strand at 5545.859 kb on - strand at 5545.859 kb on - strand at 5546.102 kb on + strand at 5546.483 kb on - strand at 5546.592 kb on + strand at 5546.613 kb on + strand at 5546.661 kb on + strand at 5546.669 kb on - strand at 5546.746 kb on - strand, within BWI76_RS26770 at 5546.757 kb on + strand, within BWI76_RS26770 at 5546.802 kb on - strand, within BWI76_RS26770 at 5546.802 kb on - strand, within BWI76_RS26770 at 5546.802 kb on - strand, within BWI76_RS26770 at 5546.970 kb on + strand, within BWI76_RS26770 at 5546.982 kb on + strand, within BWI76_RS26770 at 5547.054 kb on + strand, within BWI76_RS26770 at 5547.126 kb on - strand, within BWI76_RS26770 at 5547.412 kb on - strand, within BWI76_RS26770 at 5547.427 kb on + strand, within BWI76_RS26770 at 5547.821 kb on - strand, within BWI76_RS26770 at 5548.029 kb on + strand, within BWI76_RS26770 at 5548.161 kb on - strand, within BWI76_RS26770 at 5548.205 kb on - strand, within BWI76_RS26770 at 5548.205 kb on - strand, within BWI76_RS26770 at 5548.257 kb on + strand, within BWI76_RS26770 at 5548.340 kb on + strand, within BWI76_RS26770 at 5548.450 kb on - strand, within BWI76_RS26770 at 5548.594 kb on + strand, within BWI76_RS26770 at 5548.614 kb on + strand, within BWI76_RS26770 at 5548.787 kb on - strand, within BWI76_RS26770 at 5548.815 kb on + strand, within BWI76_RS26770 at 5548.917 kb on + strand, within BWI76_RS26770 at 5548.917 kb on + strand, within BWI76_RS26770 at 5548.925 kb on - strand, within BWI76_RS26770 at 5549.185 kb on - strand, within BWI76_RS26770 at 5549.567 kb on - strand at 5549.668 kb on + strand at 5549.771 kb on + strand at 5549.832 kb on - strand at 5549.938 kb on + strand at 5549.961 kb on - strand, within BWI76_RS26775 at 5550.115 kb on + strand, within BWI76_RS26775 at 5550.227 kb on - strand, within BWI76_RS26775 at 5550.407 kb on - strand, within BWI76_RS26775 at 5550.585 kb on - strand, within BWI76_RS26775 at 5550.594 kb on + strand, within BWI76_RS26775 at 5550.627 kb on - strand, within BWI76_RS26775 at 5550.637 kb on - strand, within BWI76_RS26775 at 5550.639 kb on - strand, within BWI76_RS26775 at 5550.726 kb on + strand, within BWI76_RS26775 at 5550.763 kb on + strand, within BWI76_RS26775 at 5550.768 kb on + strand, within BWI76_RS26775
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 5,545,562 - BWI76_RS26765 0.81 +0.2 5,545,675 + BWI76_RS26765 0.87 -0.0 5,545,735 - BWI76_RS26765 0.90 -1.8 5,545,735 - BWI76_RS26765 0.90 +1.6 5,545,756 - -0.2 5,545,795 + +2.6 5,545,859 - +2.0 5,545,859 - +1.0 5,546,102 + -0.1 5,546,483 - +1.9 5,546,592 + +0.4 5,546,613 + +1.3 5,546,661 + +1.9 5,546,669 - +2.4 5,546,746 - BWI76_RS26770 0.11 +0.4 5,546,757 + BWI76_RS26770 0.11 +0.7 5,546,802 - BWI76_RS26770 0.12 +0.8 5,546,802 - BWI76_RS26770 0.12 +1.7 5,546,802 - BWI76_RS26770 0.12 +1.3 5,546,970 + BWI76_RS26770 0.17 -0.4 5,546,982 + BWI76_RS26770 0.17 +0.7 5,547,054 + BWI76_RS26770 0.19 +0.4 5,547,126 - BWI76_RS26770 0.22 +1.2 5,547,412 - BWI76_RS26770 0.30 +1.4 5,547,427 + BWI76_RS26770 0.30 +3.2 5,547,821 - BWI76_RS26770 0.41 +0.7 5,548,029 + BWI76_RS26770 0.47 +1.0 5,548,161 - BWI76_RS26770 0.51 +0.4 5,548,205 - BWI76_RS26770 0.52 +2.0 5,548,205 - BWI76_RS26770 0.52 +0.6 5,548,257 + BWI76_RS26770 0.54 +0.0 5,548,340 + BWI76_RS26770 0.56 +1.3 5,548,450 - BWI76_RS26770 0.60 +0.8 5,548,594 + BWI76_RS26770 0.64 +1.8 5,548,614 + BWI76_RS26770 0.64 +1.3 5,548,787 - BWI76_RS26770 0.69 +0.9 5,548,815 + BWI76_RS26770 0.70 +1.6 5,548,917 + BWI76_RS26770 0.73 +0.7 5,548,917 + BWI76_RS26770 0.73 -0.4 5,548,925 - BWI76_RS26770 0.73 +0.8 5,549,185 - BWI76_RS26770 0.81 -2.3 5,549,567 - +0.8 5,549,668 + -0.2 5,549,771 + +0.6 5,549,832 - +1.3 5,549,938 + -0.8 5,549,961 - BWI76_RS26775 0.11 -0.5 5,550,115 + BWI76_RS26775 0.25 -1.6 5,550,227 - BWI76_RS26775 0.35 +0.0 5,550,407 - BWI76_RS26775 0.51 -0.8 5,550,585 - BWI76_RS26775 0.67 -1.6 5,550,594 + BWI76_RS26775 0.68 +2.6 5,550,627 - BWI76_RS26775 0.71 +0.6 5,550,637 - BWI76_RS26775 0.72 +0.3 5,550,639 - BWI76_RS26775 0.72 -1.1 5,550,726 + BWI76_RS26775 0.80 -0.8 5,550,763 + BWI76_RS26775 0.83 -1.3 5,550,768 + BWI76_RS26775 0.83 +0.3
Or see this region's nucleotide sequence