Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26620 and BWI76_RS26625 are separated by 58 nucleotides BWI76_RS26625 and BWI76_RS26630 are separated by 37 nucleotides BWI76_RS26630 and BWI76_RS26635 are separated by 111 nucleotides BWI76_RS26635 and BWI76_RS26640 are separated by 221 nucleotides BWI76_RS26640 and BWI76_RS26645 are separated by 9 nucleotides
BWI76_RS26620: BWI76_RS26620 - Fe-S biogenesis protein NfuA, at 5,511,701 to 5,512,276
_RS26620
BWI76_RS26625: BWI76_RS26625 - gluconate transporter periplasmic gluconate-binding protein, at 5,512,335 to 5,513,009
_RS26625
BWI76_RS26630: BWI76_RS26630 - pimeloyl-[acyl-carrier protein] methyl ester esterase, at 5,513,047 to 5,513,829
_RS26630
BWI76_RS26635: BWI76_RS26635 - hypothetical protein, at 5,513,941 to 5,514,210
_RS26635
BWI76_RS26640: BWI76_RS26640 - ferrous iron transporter FeoC, at 5,514,432 to 5,514,668
_RS26640
BWI76_RS26645: BWI76_RS26645 - ferrous iron transporter B, at 5,514,678 to 5,516,996
_RS26645
Position (kb) 5513
5514 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 5512.114 kb on - strand, within BWI76_RS26620 at 5512.362 kb on - strand at 5512.511 kb on + strand, within BWI76_RS26625 at 5512.734 kb on + strand, within BWI76_RS26625 at 5512.743 kb on - strand, within BWI76_RS26625 at 5512.770 kb on - strand, within BWI76_RS26625 at 5512.860 kb on + strand, within BWI76_RS26625 at 5513.030 kb on + strand at 5513.108 kb on + strand at 5513.138 kb on + strand, within BWI76_RS26630 at 5513.141 kb on - strand, within BWI76_RS26630 at 5513.324 kb on - strand, within BWI76_RS26630 at 5513.375 kb on + strand, within BWI76_RS26630 at 5513.524 kb on + strand, within BWI76_RS26630 at 5513.669 kb on + strand, within BWI76_RS26630 at 5513.797 kb on + strand at 5513.930 kb on + strand at 5513.984 kb on + strand, within BWI76_RS26635 at 5513.988 kb on + strand, within BWI76_RS26635 at 5513.996 kb on - strand, within BWI76_RS26635 at 5514.244 kb on + strand at 5514.298 kb on + strand at 5514.608 kb on - strand, within BWI76_RS26640 at 5514.764 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 5,512,114 - BWI76_RS26620 0.72 +8.7 5,512,362 - +1.6 5,512,511 + BWI76_RS26625 0.26 +0.5 5,512,734 + BWI76_RS26625 0.59 +0.3 5,512,743 - BWI76_RS26625 0.60 +1.9 5,512,770 - BWI76_RS26625 0.64 +2.3 5,512,860 + BWI76_RS26625 0.78 -0.5 5,513,030 + +1.5 5,513,108 + -0.2 5,513,138 + BWI76_RS26630 0.12 -0.8 5,513,141 - BWI76_RS26630 0.12 -0.2 5,513,324 - BWI76_RS26630 0.35 -0.3 5,513,375 + BWI76_RS26630 0.42 -1.2 5,513,524 + BWI76_RS26630 0.61 -1.6 5,513,669 + BWI76_RS26630 0.79 -1.1 5,513,797 + -0.0 5,513,930 + +3.6 5,513,984 + BWI76_RS26635 0.16 -1.9 5,513,988 + BWI76_RS26635 0.17 -0.4 5,513,996 - BWI76_RS26635 0.20 -0.7 5,514,244 + +1.5 5,514,298 + +1.1 5,514,608 - BWI76_RS26640 0.74 +0.1 5,514,764 - -1.3
Or see this region's nucleotide sequence