Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26600 and BWI76_RS26605 are separated by 101 nucleotides BWI76_RS26605 and BWI76_RS26610 are separated by 7 nucleotides BWI76_RS26610 and BWI76_RS26615 are separated by 209 nucleotides
BWI76_RS26600: BWI76_RS26600 - hypothetical protein, at 5,506,613 to 5,506,912
_RS26600
BWI76_RS26605: BWI76_RS26605 - 16S rRNA (guanine(1516)-N(2))-methyltransferase, at 5,507,014 to 5,507,793
_RS26605
BWI76_RS26610: BWI76_RS26610 - oligopeptidase A, at 5,507,801 to 5,509,843
_RS26610
BWI76_RS26615: BWI76_RS26615 - gluconate:H+ symporter <small>(from data)</small>, at 5,510,053 to 5,511,369
_RS26615
Position (kb) 5507
5508
5509
5510 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5506.820 kb on - strand, within BWI76_RS26600 at 5506.877 kb on + strand, within BWI76_RS26600 at 5506.877 kb on + strand, within BWI76_RS26600 at 5506.885 kb on - strand at 5506.919 kb on - strand at 5507.148 kb on - strand, within BWI76_RS26605 at 5507.185 kb on - strand, within BWI76_RS26605 at 5507.285 kb on + strand, within BWI76_RS26605 at 5507.290 kb on + strand, within BWI76_RS26605 at 5507.307 kb on + strand, within BWI76_RS26605 at 5507.315 kb on - strand, within BWI76_RS26605 at 5507.315 kb on - strand, within BWI76_RS26605 at 5507.315 kb on - strand, within BWI76_RS26605 at 5507.339 kb on - strand, within BWI76_RS26605 at 5507.390 kb on + strand, within BWI76_RS26605 at 5507.406 kb on + strand, within BWI76_RS26605 at 5507.464 kb on + strand, within BWI76_RS26605 at 5507.739 kb on + strand at 5507.814 kb on - strand at 5508.144 kb on + strand, within BWI76_RS26610 at 5508.147 kb on - strand, within BWI76_RS26610 at 5508.160 kb on - strand, within BWI76_RS26610 at 5508.372 kb on - strand, within BWI76_RS26610 at 5508.404 kb on + strand, within BWI76_RS26610 at 5508.424 kb on - strand, within BWI76_RS26610 at 5508.480 kb on - strand, within BWI76_RS26610 at 5508.509 kb on + strand at 5508.590 kb on - strand, within BWI76_RS26610 at 5508.729 kb on + strand, within BWI76_RS26610 at 5508.788 kb on - strand, within BWI76_RS26610 at 5508.910 kb on + strand, within BWI76_RS26610 at 5508.941 kb on - strand, within BWI76_RS26610 at 5509.142 kb on - strand, within BWI76_RS26610 at 5509.153 kb on + strand, within BWI76_RS26610 at 5509.311 kb on + strand, within BWI76_RS26610 at 5509.351 kb on - strand, within BWI76_RS26610 at 5509.412 kb on - strand, within BWI76_RS26610 at 5509.473 kb on + strand, within BWI76_RS26610 at 5509.473 kb on + strand, within BWI76_RS26610 at 5509.473 kb on + strand, within BWI76_RS26610 at 5509.478 kb on - strand, within BWI76_RS26610 at 5509.480 kb on + strand, within BWI76_RS26610 at 5509.483 kb on - strand, within BWI76_RS26610 at 5509.492 kb on - strand, within BWI76_RS26610 at 5509.515 kb on - strand, within BWI76_RS26610 at 5509.538 kb on - strand, within BWI76_RS26610 at 5509.708 kb on - strand at 5509.773 kb on + strand at 5509.854 kb on + strand at 5510.041 kb on - strand at 5510.082 kb on + strand at 5510.127 kb on + strand at 5510.368 kb on + strand, within BWI76_RS26615 at 5510.438 kb on - strand, within BWI76_RS26615 at 5510.629 kb on + strand, within BWI76_RS26615 at 5510.667 kb on + strand, within BWI76_RS26615 at 5510.687 kb on + strand, within BWI76_RS26615
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 5,506,820 - BWI76_RS26600 0.69 +0.9 5,506,877 + BWI76_RS26600 0.88 +1.1 5,506,877 + BWI76_RS26600 0.88 -0.6 5,506,885 - -0.2 5,506,919 - -0.8 5,507,148 - BWI76_RS26605 0.17 -1.2 5,507,185 - BWI76_RS26605 0.22 -0.4 5,507,285 + BWI76_RS26605 0.35 +0.9 5,507,290 + BWI76_RS26605 0.35 +0.3 5,507,307 + BWI76_RS26605 0.38 -0.0 5,507,315 - BWI76_RS26605 0.39 -3.0 5,507,315 - BWI76_RS26605 0.39 +3.0 5,507,315 - BWI76_RS26605 0.39 -1.1 5,507,339 - BWI76_RS26605 0.42 -0.3 5,507,390 + BWI76_RS26605 0.48 +0.3 5,507,406 + BWI76_RS26605 0.50 -3.7 5,507,464 + BWI76_RS26605 0.58 -2.0 5,507,739 + -0.2 5,507,814 - -1.8 5,508,144 + BWI76_RS26610 0.17 +0.8 5,508,147 - BWI76_RS26610 0.17 -0.9 5,508,160 - BWI76_RS26610 0.18 -2.0 5,508,372 - BWI76_RS26610 0.28 -1.2 5,508,404 + BWI76_RS26610 0.30 -1.4 5,508,424 - BWI76_RS26610 0.30 -1.5 5,508,480 - BWI76_RS26610 0.33 -1.9 5,508,509 + +2.0 5,508,590 - BWI76_RS26610 0.39 -2.2 5,508,729 + BWI76_RS26610 0.45 -3.1 5,508,788 - BWI76_RS26610 0.48 -0.5 5,508,910 + BWI76_RS26610 0.54 -2.1 5,508,941 - BWI76_RS26610 0.56 +0.3 5,509,142 - BWI76_RS26610 0.66 -1.8 5,509,153 + BWI76_RS26610 0.66 -1.5 5,509,311 + BWI76_RS26610 0.74 -2.3 5,509,351 - BWI76_RS26610 0.76 -0.3 5,509,412 - BWI76_RS26610 0.79 -1.3 5,509,473 + BWI76_RS26610 0.82 -1.3 5,509,473 + BWI76_RS26610 0.82 +1.1 5,509,473 + BWI76_RS26610 0.82 -1.4 5,509,478 - BWI76_RS26610 0.82 -1.8 5,509,480 + BWI76_RS26610 0.82 -3.2 5,509,483 - BWI76_RS26610 0.82 -1.7 5,509,492 - BWI76_RS26610 0.83 -1.4 5,509,515 - BWI76_RS26610 0.84 -0.9 5,509,538 - BWI76_RS26610 0.85 -2.8 5,509,708 - -2.5 5,509,773 + -0.0 5,509,854 + -1.8 5,510,041 - +1.2 5,510,082 + -0.5 5,510,127 + -1.3 5,510,368 + BWI76_RS26615 0.24 +2.1 5,510,438 - BWI76_RS26615 0.29 +0.0 5,510,629 + BWI76_RS26615 0.44 +0.1 5,510,667 + BWI76_RS26615 0.47 -0.6 5,510,687 + BWI76_RS26615 0.48 +0.6
Or see this region's nucleotide sequence