Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25205

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25200 and BWI76_RS25205 are separated by 54 nucleotidesBWI76_RS25205 and BWI76_RS25210 are separated by 135 nucleotidesBWI76_RS25210 and BWI76_RS25215 overlap by 1 nucleotides BWI76_RS25200: BWI76_RS25200 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 5,242,406 to 5,243,665 _RS25200 BWI76_RS25205: BWI76_RS25205 - hypothetical protein, at 5,243,720 to 5,243,974 _RS25205 BWI76_RS25210: BWI76_RS25210 - sulfate transporter, at 5,244,110 to 5,244,400 _RS25210 BWI76_RS25215: BWI76_RS25215 - phospholipid-binding protein MlaC, at 5,244,400 to 5,245,035 _RS25215 Position (kb) 5243 5244Strain fitness (log2 ratio) -1 0 1 2at 5243.761 kb on - strand, within BWI76_RS25205at 5244.001 kb on - strandat 5244.019 kb on - strandat 5244.039 kb on - strandat 5244.176 kb on + strand, within BWI76_RS25210at 5244.381 kb on + strandat 5244.440 kb on + strandat 5244.624 kb on + strand, within BWI76_RS25215at 5244.666 kb on - strand, within BWI76_RS25215at 5244.677 kb on - strand, within BWI76_RS25215at 5244.737 kb on - strand, within BWI76_RS25215at 5244.890 kb on + strand, within BWI76_RS25215at 5244.956 kb on - strand, within BWI76_RS25215

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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5,243,761 - BWI76_RS25205 0.16 +1.8
5,244,001 - +0.8
5,244,019 - +0.5
5,244,039 - +0.8
5,244,176 + BWI76_RS25210 0.23 +0.4
5,244,381 + +0.4
5,244,440 + -0.3
5,244,624 + BWI76_RS25215 0.35 +1.1
5,244,666 - BWI76_RS25215 0.42 +0.4
5,244,677 - BWI76_RS25215 0.44 +0.9
5,244,737 - BWI76_RS25215 0.53 +0.5
5,244,890 + BWI76_RS25215 0.77 -0.6
5,244,956 - BWI76_RS25215 0.87 +0.1

Or see this region's nucleotide sequence