Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24990 and BWI76_RS24995 are separated by 12 nucleotides BWI76_RS24995 and BWI76_RS25000 are separated by 11 nucleotides BWI76_RS25000 and BWI76_RS25005 overlap by 8 nucleotides
BWI76_RS24990: BWI76_RS24990 - Na+-transporting malonate decarboxylase, carboxybiotin decarboxylase subunit, at 5,203,951 to 5,205,159
_RS24990
BWI76_RS24995: BWI76_RS24995 - malonate decarboxylase subunit alpha, at 5,205,172 to 5,206,839
_RS24995
BWI76_RS25000: BWI76_RS25000 - malonate decarboxylase acyl carrier protein, at 5,206,851 to 5,207,183
_RS25000
BWI76_RS25005: BWI76_RS25005 - biotin-independent malonate decarboxylase subunit beta, at 5,207,176 to 5,208,105
_RS25005
Position (kb) 5205
5206
5207 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5204.189 kb on + strand, within BWI76_RS24990 at 5204.198 kb on + strand, within BWI76_RS24990 at 5204.201 kb on - strand, within BWI76_RS24990 at 5204.208 kb on + strand, within BWI76_RS24990 at 5204.208 kb on + strand, within BWI76_RS24990 at 5204.208 kb on + strand, within BWI76_RS24990 at 5204.208 kb on + strand, within BWI76_RS24990 at 5204.208 kb on + strand, within BWI76_RS24990 at 5204.208 kb on + strand, within BWI76_RS24990 at 5204.208 kb on + strand, within BWI76_RS24990 at 5204.208 kb on + strand, within BWI76_RS24990 at 5204.213 kb on + strand, within BWI76_RS24990 at 5204.216 kb on - strand, within BWI76_RS24990 at 5204.216 kb on - strand, within BWI76_RS24990 at 5204.216 kb on - strand, within BWI76_RS24990 at 5204.216 kb on - strand, within BWI76_RS24990 at 5204.216 kb on - strand, within BWI76_RS24990 at 5204.216 kb on - strand, within BWI76_RS24990 at 5204.223 kb on + strand, within BWI76_RS24990 at 5204.231 kb on - strand, within BWI76_RS24990 at 5204.250 kb on - strand, within BWI76_RS24990 at 5204.274 kb on + strand, within BWI76_RS24990 at 5204.276 kb on + strand, within BWI76_RS24990 at 5204.326 kb on - strand, within BWI76_RS24990 at 5204.407 kb on + strand, within BWI76_RS24990 at 5204.796 kb on - strand, within BWI76_RS24990 at 5204.801 kb on - strand, within BWI76_RS24990 at 5204.846 kb on - strand, within BWI76_RS24990 at 5205.004 kb on + strand, within BWI76_RS24990 at 5205.051 kb on + strand at 5205.294 kb on - strand at 5205.419 kb on + strand, within BWI76_RS24995 at 5205.437 kb on - strand, within BWI76_RS24995 at 5205.563 kb on + strand, within BWI76_RS24995 at 5205.571 kb on - strand, within BWI76_RS24995 at 5205.648 kb on + strand, within BWI76_RS24995 at 5205.691 kb on + strand, within BWI76_RS24995 at 5206.068 kb on + strand, within BWI76_RS24995 at 5206.137 kb on + strand, within BWI76_RS24995 at 5206.152 kb on + strand, within BWI76_RS24995 at 5206.152 kb on + strand, within BWI76_RS24995 at 5206.155 kb on - strand, within BWI76_RS24995 at 5206.192 kb on + strand, within BWI76_RS24995 at 5206.376 kb on - strand, within BWI76_RS24995 at 5206.641 kb on - strand, within BWI76_RS24995 at 5206.684 kb on + strand at 5206.806 kb on + strand at 5206.812 kb on - strand at 5206.854 kb on + strand at 5207.092 kb on - strand, within BWI76_RS25000 at 5207.131 kb on + strand, within BWI76_RS25000 at 5207.151 kb on - strand at 5207.296 kb on + strand, within BWI76_RS25005 at 5207.301 kb on + strand, within BWI76_RS25005 at 5207.339 kb on + strand, within BWI76_RS25005 at 5207.347 kb on - strand, within BWI76_RS25005 at 5207.549 kb on - strand, within BWI76_RS25005 at 5207.650 kb on - strand, within BWI76_RS25005
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 5,204,189 + BWI76_RS24990 0.20 +0.4 5,204,198 + BWI76_RS24990 0.20 -1.4 5,204,201 - BWI76_RS24990 0.21 +0.6 5,204,208 + BWI76_RS24990 0.21 +2.2 5,204,208 + BWI76_RS24990 0.21 -0.3 5,204,208 + BWI76_RS24990 0.21 +0.7 5,204,208 + BWI76_RS24990 0.21 +1.2 5,204,208 + BWI76_RS24990 0.21 +1.0 5,204,208 + BWI76_RS24990 0.21 -1.5 5,204,208 + BWI76_RS24990 0.21 +0.3 5,204,208 + BWI76_RS24990 0.21 -1.1 5,204,213 + BWI76_RS24990 0.22 +0.1 5,204,216 - BWI76_RS24990 0.22 -0.6 5,204,216 - BWI76_RS24990 0.22 +0.2 5,204,216 - BWI76_RS24990 0.22 +0.8 5,204,216 - BWI76_RS24990 0.22 +0.6 5,204,216 - BWI76_RS24990 0.22 -0.7 5,204,216 - BWI76_RS24990 0.22 +1.5 5,204,223 + BWI76_RS24990 0.22 -2.5 5,204,231 - BWI76_RS24990 0.23 -0.8 5,204,250 - BWI76_RS24990 0.25 -0.8 5,204,274 + BWI76_RS24990 0.27 -1.3 5,204,276 + BWI76_RS24990 0.27 +0.1 5,204,326 - BWI76_RS24990 0.31 +0.7 5,204,407 + BWI76_RS24990 0.38 -0.6 5,204,796 - BWI76_RS24990 0.70 +1.0 5,204,801 - BWI76_RS24990 0.70 -2.1 5,204,846 - BWI76_RS24990 0.74 +0.1 5,205,004 + BWI76_RS24990 0.87 +0.9 5,205,051 + -1.9 5,205,294 - -0.6 5,205,419 + BWI76_RS24995 0.15 -1.7 5,205,437 - BWI76_RS24995 0.16 -0.1 5,205,563 + BWI76_RS24995 0.23 -0.4 5,205,571 - BWI76_RS24995 0.24 -0.3 5,205,648 + BWI76_RS24995 0.29 +1.2 5,205,691 + BWI76_RS24995 0.31 -0.4 5,206,068 + BWI76_RS24995 0.54 +0.1 5,206,137 + BWI76_RS24995 0.58 +2.4 5,206,152 + BWI76_RS24995 0.59 +1.9 5,206,152 + BWI76_RS24995 0.59 +0.1 5,206,155 - BWI76_RS24995 0.59 -0.5 5,206,192 + BWI76_RS24995 0.61 -0.3 5,206,376 - BWI76_RS24995 0.72 -0.1 5,206,641 - BWI76_RS24995 0.88 -0.2 5,206,684 + +1.3 5,206,806 + -0.8 5,206,812 - +1.4 5,206,854 + -0.1 5,207,092 - BWI76_RS25000 0.72 +0.4 5,207,131 + BWI76_RS25000 0.84 -0.0 5,207,151 - +0.7 5,207,296 + BWI76_RS25005 0.13 +0.4 5,207,301 + BWI76_RS25005 0.13 -0.6 5,207,339 + BWI76_RS25005 0.18 +0.9 5,207,347 - BWI76_RS25005 0.18 +0.0 5,207,549 - BWI76_RS25005 0.40 +0.7 5,207,650 - BWI76_RS25005 0.51 -0.3
Or see this region's nucleotide sequence