Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23805

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23800 and BWI76_RS23805 are separated by 155 nucleotidesBWI76_RS23805 and BWI76_RS23810 are separated by 5 nucleotidesBWI76_RS23810 and BWI76_RS23815 are separated by 22 nucleotides BWI76_RS23800: BWI76_RS23800 - peptide chain release factor 2, at 4,968,113 to 4,969,141 _RS23800 BWI76_RS23805: BWI76_RS23805 - ssDNA exonuclease RecJ, at 4,969,297 to 4,971,030 _RS23805 BWI76_RS23810: BWI76_RS23810 - bifunctional protein-disulfide isomerase/oxidoreductase DsbC, at 4,971,036 to 4,971,749 _RS23810 BWI76_RS23815: BWI76_RS23815 - site-specific tyrosine recombinase XerD, at 4,971,772 to 4,972,668 _RS23815 Position (kb) 4969 4970 4971 4972Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4969.293 kb on + strandat 4969.563 kb on + strand, within BWI76_RS23805at 4969.772 kb on + strand, within BWI76_RS23805at 4969.831 kb on + strand, within BWI76_RS23805at 4969.846 kb on - strand, within BWI76_RS23805at 4970.012 kb on - strand, within BWI76_RS23805at 4970.039 kb on - strand, within BWI76_RS23805at 4970.088 kb on - strand, within BWI76_RS23805at 4970.259 kb on - strand, within BWI76_RS23805at 4970.285 kb on - strand, within BWI76_RS23805at 4970.504 kb on - strand, within BWI76_RS23805at 4970.575 kb on - strand, within BWI76_RS23805at 4970.635 kb on - strand, within BWI76_RS23805at 4970.820 kb on - strand, within BWI76_RS23805at 4970.866 kb on + strandat 4970.879 kb on - strandat 4970.879 kb on - strandat 4971.158 kb on + strand, within BWI76_RS23810at 4971.385 kb on - strand, within BWI76_RS23810at 4971.469 kb on + strandat 4971.702 kb on + strandat 4971.711 kb on - strandat 4971.838 kb on - strandat 4971.856 kb on + strandat 4971.972 kb on + strand, within BWI76_RS23815

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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4,969,293 + +0.8
4,969,563 + BWI76_RS23805 0.15 -1.1
4,969,772 + BWI76_RS23805 0.27 -0.6
4,969,831 + BWI76_RS23805 0.31 -2.0
4,969,846 - BWI76_RS23805 0.32 -0.5
4,970,012 - BWI76_RS23805 0.41 -1.1
4,970,039 - BWI76_RS23805 0.43 -0.6
4,970,088 - BWI76_RS23805 0.46 +0.3
4,970,259 - BWI76_RS23805 0.55 -0.9
4,970,285 - BWI76_RS23805 0.57 +0.2
4,970,504 - BWI76_RS23805 0.70 -0.3
4,970,575 - BWI76_RS23805 0.74 -1.0
4,970,635 - BWI76_RS23805 0.77 -0.1
4,970,820 - BWI76_RS23805 0.88 +0.3
4,970,866 + +3.1
4,970,879 - -0.6
4,970,879 - +0.1
4,971,158 + BWI76_RS23810 0.17 -0.1
4,971,385 - BWI76_RS23810 0.49 +1.1
4,971,469 + +0.5
4,971,702 + +1.7
4,971,711 - +0.4
4,971,838 - -1.2
4,971,856 + -0.7
4,971,972 + BWI76_RS23815 0.22 +0.4

Or see this region's nucleotide sequence