Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20985 and BWI76_RS20990 are separated by 3 nucleotides BWI76_RS20990 and BWI76_RS20995 are separated by 177 nucleotides
BWI76_RS20985: BWI76_RS20985 - glycosyl hydrolase family protein, at 4,387,299 to 4,388,396
_RS20985
BWI76_RS20990: BWI76_RS20990 - hypothetical protein, at 4,388,400 to 4,390,073
_RS20990
BWI76_RS20995: BWI76_RS20995 - RNA degradosome polyphosphate kinase, at 4,390,251 to 4,392,311
_RS20995
Position (kb)
4388
4389
4390
4391 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4387.486 kb on + strand, within BWI76_RS20985 at 4387.486 kb on + strand, within BWI76_RS20985 at 4387.494 kb on - strand, within BWI76_RS20985 at 4387.650 kb on + strand, within BWI76_RS20985 at 4387.797 kb on - strand, within BWI76_RS20985 at 4387.965 kb on - strand, within BWI76_RS20985 at 4388.299 kb on + strand at 4388.376 kb on + strand at 4388.384 kb on - strand at 4388.384 kb on - strand at 4388.643 kb on - strand, within BWI76_RS20990 at 4388.645 kb on + strand, within BWI76_RS20990 at 4388.841 kb on + strand, within BWI76_RS20990 at 4388.843 kb on - strand, within BWI76_RS20990 at 4389.159 kb on - strand, within BWI76_RS20990 at 4389.189 kb on - strand, within BWI76_RS20990 at 4389.225 kb on + strand, within BWI76_RS20990 at 4389.326 kb on + strand, within BWI76_RS20990 at 4389.339 kb on - strand, within BWI76_RS20990 at 4389.339 kb on - strand, within BWI76_RS20990 at 4389.526 kb on - strand, within BWI76_RS20990 at 4389.743 kb on - strand, within BWI76_RS20990 at 4389.843 kb on - strand, within BWI76_RS20990 at 4389.843 kb on - strand, within BWI76_RS20990 at 4389.860 kb on + strand, within BWI76_RS20990 at 4389.868 kb on - strand, within BWI76_RS20990 at 4389.923 kb on + strand at 4389.931 kb on + strand at 4389.974 kb on + strand at 4389.997 kb on + strand at 4390.000 kb on - strand at 4390.049 kb on + strand at 4390.231 kb on - strand at 4390.241 kb on - strand at 4390.246 kb on - strand at 4390.246 kb on - strand at 4390.446 kb on + strand at 4390.446 kb on + strand at 4390.476 kb on - strand, within BWI76_RS20995 at 4390.617 kb on - strand, within BWI76_RS20995 at 4391.043 kb on - strand, within BWI76_RS20995
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 4,387,486 + BWI76_RS20985 0.17 +2.5 4,387,486 + BWI76_RS20985 0.17 +0.1 4,387,494 - BWI76_RS20985 0.18 -0.4 4,387,650 + BWI76_RS20985 0.32 -0.6 4,387,797 - BWI76_RS20985 0.45 -0.7 4,387,965 - BWI76_RS20985 0.61 -0.5 4,388,299 + +0.3 4,388,376 + -0.9 4,388,384 - +0.6 4,388,384 - -0.5 4,388,643 - BWI76_RS20990 0.15 -0.7 4,388,645 + BWI76_RS20990 0.15 -1.0 4,388,841 + BWI76_RS20990 0.26 -0.9 4,388,843 - BWI76_RS20990 0.26 -0.4 4,389,159 - BWI76_RS20990 0.45 +0.0 4,389,189 - BWI76_RS20990 0.47 -1.3 4,389,225 + BWI76_RS20990 0.49 -1.5 4,389,326 + BWI76_RS20990 0.55 +0.1 4,389,339 - BWI76_RS20990 0.56 -0.1 4,389,339 - BWI76_RS20990 0.56 -0.0 4,389,526 - BWI76_RS20990 0.67 -0.7 4,389,743 - BWI76_RS20990 0.80 +0.7 4,389,843 - BWI76_RS20990 0.86 -0.9 4,389,843 - BWI76_RS20990 0.86 +1.1 4,389,860 + BWI76_RS20990 0.87 +0.2 4,389,868 - BWI76_RS20990 0.88 -0.4 4,389,923 + -1.2 4,389,931 + +0.3 4,389,974 + +0.5 4,389,997 + +0.3 4,390,000 - +0.5 4,390,049 + +1.8 4,390,231 - +0.9 4,390,241 - -1.9 4,390,246 - -3.9 4,390,246 - -1.9 4,390,446 + -2.8 4,390,446 + -0.5 4,390,476 - BWI76_RS20995 0.11 -2.6 4,390,617 - BWI76_RS20995 0.18 -0.5 4,391,043 - BWI76_RS20995 0.38 -1.5
Or see this region's nucleotide sequence