Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20405 and BWI76_RS20410 are separated by 158 nucleotides BWI76_RS20410 and BWI76_RS20415 are separated by 55 nucleotides BWI76_RS20415 and BWI76_RS20420 are separated by 31 nucleotides BWI76_RS20420 and BWI76_RS20425 overlap by 1 nucleotides
BWI76_RS20405: BWI76_RS20405 - beta-ketoacyl-[acyl-carrier-protein] synthase I, at 4,271,411 to 4,272,628
_RS20405
BWI76_RS20410: BWI76_RS20410 - bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC, at 4,272,787 to 4,274,775
_RS20410
BWI76_RS20415: BWI76_RS20415 - hypothetical protein, at 4,274,831 to 4,275,109
_RS20415
BWI76_RS20420: BWI76_RS20420 - elongation factor P hydroxylase, at 4,275,141 to 4,275,689
_RS20420
BWI76_RS20425: BWI76_RS20425 - hypothetical protein, at 4,275,689 to 4,276,498
_RS20425
Position (kb) 4272
4273
4274
4275 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4273.075 kb on - strand, within BWI76_RS20410 at 4273.129 kb on - strand, within BWI76_RS20410 at 4273.202 kb on + strand, within BWI76_RS20410 at 4273.381 kb on + strand, within BWI76_RS20410 at 4273.742 kb on + strand, within BWI76_RS20410 at 4273.929 kb on + strand, within BWI76_RS20410 at 4274.240 kb on + strand, within BWI76_RS20410 at 4274.443 kb on - strand, within BWI76_RS20410 at 4274.452 kb on + strand, within BWI76_RS20410 at 4274.627 kb on - strand at 4274.885 kb on - strand, within BWI76_RS20415 at 4275.178 kb on - strand at 4275.258 kb on + strand, within BWI76_RS20420
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 4,273,075 - BWI76_RS20410 0.14 +0.3 4,273,129 - BWI76_RS20410 0.17 -1.6 4,273,202 + BWI76_RS20410 0.21 -1.7 4,273,381 + BWI76_RS20410 0.30 -1.4 4,273,742 + BWI76_RS20410 0.48 -0.8 4,273,929 + BWI76_RS20410 0.57 -2.0 4,274,240 + BWI76_RS20410 0.73 -1.8 4,274,443 - BWI76_RS20410 0.83 -0.8 4,274,452 + BWI76_RS20410 0.84 +2.5 4,274,627 - -1.8 4,274,885 - BWI76_RS20415 0.19 +1.6 4,275,178 - +0.7 4,275,258 + BWI76_RS20420 0.21 -0.2
Or see this region's nucleotide sequence