Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19540 and BWI76_RS19545 are separated by 119 nucleotides BWI76_RS19545 and BWI76_RS19550 are separated by 96 nucleotides BWI76_RS19550 and BWI76_RS19555 are separated by 17 nucleotides
BWI76_RS19540: BWI76_RS19540 - multidrug transporter permease, at 4,083,838 to 4,085,280
_RS19540
BWI76_RS19545: BWI76_RS19545 - type 11 methyltransferase, at 4,085,400 to 4,086,134
_RS19545
BWI76_RS19550: BWI76_RS19550 - phospho-cellobiase, at 4,086,231 to 4,087,625
_RS19550
BWI76_RS19555: BWI76_RS19555 - Beta-glucoside phosphotransferase system, IIA/IIB/IIC components (from data) , at 4,087,643 to 4,089,502
_RS19555
Position (kb)
4086
4087
4088 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4086.341 kb on + strand at 4086.397 kb on - strand, within BWI76_RS19550 at 4086.517 kb on + strand, within BWI76_RS19550 at 4086.517 kb on + strand, within BWI76_RS19550 at 4087.281 kb on - strand, within BWI76_RS19550 at 4087.486 kb on + strand at 4087.523 kb on + strand at 4087.533 kb on + strand at 4087.574 kb on + strand at 4088.067 kb on - strand, within BWI76_RS19555 at 4088.172 kb on - strand, within BWI76_RS19555 at 4088.207 kb on + strand, within BWI76_RS19555 at 4088.365 kb on - strand, within BWI76_RS19555
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 4,086,341 + +0.6 4,086,397 - BWI76_RS19550 0.12 +0.2 4,086,517 + BWI76_RS19550 0.21 +0.2 4,086,517 + BWI76_RS19550 0.21 +3.1 4,087,281 - BWI76_RS19550 0.75 +0.8 4,087,486 + +0.5 4,087,523 + +0.2 4,087,533 + +1.1 4,087,574 + -0.7 4,088,067 - BWI76_RS19555 0.23 -0.4 4,088,172 - BWI76_RS19555 0.28 +0.1 4,088,207 + BWI76_RS19555 0.30 -0.4 4,088,365 - BWI76_RS19555 0.39 +0.9
Or see this region's nucleotide sequence